Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,013	C→T	A172T (GCA→ACA)	fnr ←	DNA‑binding transcriptional dual regulator FNR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,013	0	C	T	100.0%	186.0 / NA	53	A172T (GCA→ACA)	fnr	DNA‑binding transcriptional dual regulator FNR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)

ACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCG  >  NC_000913/1398879‑1399149                                                                                                                                       |                                                                                                                                         aCCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCa                                                                                                                                     >  1:232840/1‑140 (MQ=255)            ggCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCg                                                                                                                          >  1:362830/1‑140 (MQ=255)             gCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCgg                                                                                                                         >  2:1331521/1‑140 (MQ=255)                       tCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTttctt                                                                                                               >  1:50387/1‑140 (MQ=255)                           ccGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGac                                                                                                           <  1:1027438/140‑1 (MQ=255)                           ccGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGac                                                                                                           <  2:557385/140‑1 (MQ=255)                            cGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGaca                                                                                                          <  2:999295/140‑1 (MQ=255)                             gTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGacaa                                                                                                         >  1:978330/1‑140 (MQ=255)                                aGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcag                                                                                                      >  2:24954/1‑140 (MQ=255)                                aGGCCCAGATAGTTACCGATATCGCCACGAATCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcag                                                                                                      <  2:495793/140‑1 (MQ=255)                                  gCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcagca                                                                                                    <  1:246344/140‑1 (MQ=255)                                  gCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcagca                                                                                                    <  1:24954/140‑1 (MQ=255)                                  gCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcagca                                                                                                    <  2:974835/140‑1 (MQ=255)                                  gCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcagca                                                                                                    <  2:50387/140‑1 (MQ=255)                                  gCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcagca                                                                                                    >  2:899447/1‑140 (MQ=255)                                  gCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcagca                                                                                                    <  2:259582/140‑1 (MQ=255)                                   cccAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcagcag                                                                                                   >  2:378138/1‑140 (MQ=255)                                            ttACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTc                                                                                          <  1:539799/140‑1 (MQ=255)                                            ttACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTc                                                                                          >  1:1104151/1‑140 (MQ=255)                                            ttACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACAAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATc                                                                                               <  1:1376698/135‑1 (MQ=255)                                            ttACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACAAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATc                                                                                               >  2:1376698/1‑135 (MQ=255)                                              aCCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGa                                                                                                 <  2:932912/131‑1 (MQ=255)                                              aCCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGa                                                                                                 >  1:932912/1‑131 (MQ=255)                                                 gATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGAt                                                                                     <  1:56316/140‑1 (MQ=255)                                                  atatCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATc                                                                                    >  2:727607/1‑140 (MQ=255)                                                  atatCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATc                                                                                    >  1:660501/1‑140 (MQ=255)                                                     tCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGcc                                                                                 <  2:257113/140‑1 (MQ=255)                                                     tCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGcc                                                                                 <  2:978330/140‑1 (MQ=255)                                                      cGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCt                                                                                >  2:625109/1‑140 (MQ=255)                                                      cGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCt                                                                                >  2:584857/1‑140 (MQ=255)                                                                cATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCAc                                                                      >  2:239902/1‑140 (MQ=255)                                                                 aTCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCAcc                                                                     <  1:239902/140‑1 (MQ=255)                                                                   cGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGc                                                                   >  1:819652/1‑140 (MQ=255)                                                                       aGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCtcat                                                               >  2:13762/1‑140 (MQ=255)                                                                       aGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCtcat                                                               >  1:755256/1‑140 (MQ=255)                                                                         gCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCtcatca                                                             <  1:1165651/140‑1 (MQ=255)                                                                         gCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCtcatca                                                             >  1:1131512/1‑140 (MQ=255)                                                                            gAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGAc                                                          >  1:1060981/1‑140 (MQ=255)                                                                                  aCGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGcatca                                                    <  1:899447/140‑1 (MQ=255)                                                                                  aCGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGcatca                                                    <  1:13762/140‑1 (MQ=255)                                                                                       gggAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATctgc                                               <  2:1312535/140‑1 (MQ=255)                                                                                       gggAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATctg                                                >  2:1215516/1‑139 (MQ=255)                                                                                       gggAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATctg                                                <  1:1215516/139‑1 (MQ=255)                                                                                               ccGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAg                                       >  2:1223278/1‑140 (MQ=255)                                                                                                       ggCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCa                               <  2:755256/140‑1 (MQ=255)                                                                                                       ggCAAAACGACGGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCa                               <  1:727607/140‑1 (MQ=255)                                                                                                             acgacgGGACAGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTAc                         <  2:819652/140‑1 (MQ=255)                                                                                                                       aGGTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATc               <  2:362830/140‑1 (MQ=255)                                                                                                                         gTTGTAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGt             >  2:931414/1‑140 (MQ=255)                                                                                                                             tAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAg         <  2:660501/140‑1 (MQ=255)                                                                                                                             tAGATGAATGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAg         >  1:1296833/1‑140 (MQ=255)                                                                                                                                   aaTGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTc   >  2:921235/1‑140 (MQ=255)                                                                                                                                    aTGTAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCg  >  2:267054/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         ACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCG  >  NC_000913/1398879‑1399149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑