Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,866,348	T→C	Y42C (TAC→TGC)	mipA ←	scaffolding protein that interacts with murein polymerase and murein hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,866,348	0	T	C	100.0%	226.3 / NA	63	Y42C (TAC→TGC)	mipA	scaffolding protein that interacts with murein polymerase and murein hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/33);  total (30/33)

AAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCA  >  NC_000913/1866212‑1866481                                                                                                                                         |                                                                                                                                      aaGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAAt                                                                                                                                    <  1:876881/140‑1 (MQ=255) aaGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAAt                                                                                                                                    <  1:362776/140‑1 (MQ=255) aaGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAAt                                                                                                                                    <  1:1405697/140‑1 (MQ=255) aaGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAAt                                                                                                                                    <  2:458537/140‑1 (MQ=255) aaGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAAt                                                                                                                                    <  2:171118/140‑1 (MQ=255)    cggcgACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCtt                                                                                                                                 >  2:471934/1‑140 (MQ=255)      gcgACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTta                                                                                                                               >  1:535271/1‑140 (MQ=255)        gACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTACATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTtata                                                                                                                             <  1:572962/140‑1 (MQ=255)                 gCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTc                                                                                                                    >  1:79434/1‑140 (MQ=255)                    gTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAAc                                                                                                                 <  2:432530/140‑1 (MQ=255)                     tAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACg                                                                                                                <  2:782196/140‑1 (MQ=255)                          gAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACAcc                                                                                                           <  1:1000841/140‑1 (MQ=255)                               tttATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGc                                                                                                      >  1:168117/1‑140 (MQ=255)                                 tATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCt                                                                                                    >  2:1246338/1‑140 (MQ=255)                                       ttGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGccc                                                                                              >  1:567523/1‑140 (MQ=255)                                         gCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAg                                                                                            <  2:168117/140‑1 (MQ=255)                                                    caGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTAc                                                                                 >  2:555245/1‑140 (MQ=255)                                                     aGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTAcc                                                                                <  1:555245/140‑1 (MQ=255)                                                            aaCCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCg                                                                         >  1:1399973/1‑140 (MQ=255)                                                            aaCCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCg                                                                         >  2:1031135/1‑140 (MQ=255)                                                                 ccaccTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCtt                                                                              >  2:1120615/1‑130 (MQ=255)                                                                 ccaccTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCtt                                                                              <  1:1120615/130‑1 (MQ=255)                                                                 ccaccTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTc                                                                             >  1:767385/1‑131 (MQ=255)                                                                 ccaccTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTc                                                                             <  2:767385/131‑1 (MQ=255)                                                                 ccaccTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTgcgc                                                                    >  1:987619/1‑140 (MQ=255)                                                                   accTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTa                                                                  >  2:751145/1‑140 (MQ=255)                                                                   accTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTa                                                                  <  1:207227/140‑1 (MQ=255)                                                                    ccTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTAc                                                                 >  1:723881/1‑140 (MQ=255)                                                                     cTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACg                                                                >  1:1186717/1‑140 (MQ=255)                                                                           cACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCa                                                          <  2:876153/140‑1 (MQ=255)                                                                            aCGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAg                                                         >  1:1051902/1‑140 (MQ=255)                                                                             cGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGa                                                        >  2:638041/1‑140 (MQ=255)                                                                              ggAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCTCAGGGAAAATTTACCTTCAGCGTGCGCt                                                                   >  1:1381503/1‑128 (MQ=255)                                                                              ggAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCTCAGGGAAAATTTACCTTCAGCGTGCGCt                                                                   <  2:1381503/128‑1 (MQ=255)                                                                               gAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACg                                                      <  1:1246338/140‑1 (MQ=255)                                                                               gAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACg                                                      <  2:1186717/140‑1 (MQ=255)                                                                               gAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACg                                                      <  1:1031135/140‑1 (MQ=255)                                                                                       aGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACACCTGCAGACGTTGCGATa                                              <  2:535271/140‑1 (MQ=255)                                                                                          tATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAg                                            >  1:866016/1‑139 (MQ=255)                                                                                          tATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAg                                            <  2:866016/139‑1 (MQ=255)                                                                                          tATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGc                                           >  1:1359622/1‑140 (MQ=255)                                                                                                   cATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGt                                  >  2:670117/1‑140 (MQ=255)                                                                                                            ttACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAg                                   >  1:448855/1‑130 (MQ=255)                                                                                                            ttACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAg                                   <  2:448855/130‑1 (MQ=255)                                                                                                             tACCGGTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAagtt                        >  2:1018703/1‑140 (MQ=255)                                                                                                                  gTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAagtttgagt                   <  1:1231078/140‑1 (MQ=255)                                                                                                                  gTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAagtttgagt                   <  1:1018703/140‑1 (MQ=255)                                                                                                                  gTACTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAagtttgagt                   <  2:1051902/140‑1 (MQ=255)                                                                                                                     cTGGGTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAagtttgagtttg                >  2:1033295/1‑140 (MQ=255)                                                                                                                       gggTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGt              >  1:1420206/1‑140 (MQ=255)                                                                                                                       gggTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGt              >  2:822806/1‑140 (MQ=255)                                                                                                                       gggTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGt              <  1:456044/140‑1 (MQ=255)                                                                                                                       gggTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGt              <  1:471934/140‑1 (MQ=255)                                                                                                                        ggTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAagttt                       >  2:1220909/1‑130 (MQ=255)                                                                                                                        ggTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAagttt                       <  1:1220909/130‑1 (MQ=255)                                                                                                                         gTAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTca            <  2:255768/140‑1 (MQ=255)                                                                                                                          tAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTcac           <  2:1359622/140‑1 (MQ=255)                                                                                                                          tAAACATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTcac           >  1:145263/1‑140 (MQ=255)                                                                                                                             aCATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGt              >  2:1175127/1‑134 (MQ=255)                                                                                                                             aCATCGGTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGt              <  1:1175127/134‑1 (MQ=255)                                                                                                                                  ggTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGt              >  2:1161082/1‑129 (MQ=255)                                                                                                                                  ggTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGt              <  1:1161082/129‑1 (MQ=255)                                                                                                                                   gTATCGCAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCa  <  2:79434/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      AAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTACTGGGTAAACATCGGTATCGTAATCTTTATATGGGTGTTCAACGACACCTACGCCTGCGCCCAGGGAAAATTTACCTTCAGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCA  >  NC_000913/1866212‑1866481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑