Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,840	G→A	P51P (CCG→CCA)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,840	0	G	A	98.9%	316.3 / NA	89	P51P (CCG→CCA)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (42/46);  minor base C (0/1);  total (42/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGA  >  NC_000913/1905703‑1905968                                                                                                                                          |                                                                                                                                 aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACt                                                                                                                                >  1:1255542/1‑140 (MQ=255)  cTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTg                                                                                                                               >  2:297192/1‑140 (MQ=255)    gTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGAt                                                                                                                             <  2:724711/140‑1 (MQ=255)    gTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGAt                                                                                                                             <  2:240741/140‑1 (MQ=255)    gTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGAt                                                                                                                             <  2:1265094/140‑1 (MQ=255)    gTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGAt                                                                                                                             <  1:55591/140‑1 (MQ=255)    gTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGAt                                                                                                                             <  1:422388/140‑1 (MQ=255)    gTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGAt                                                                                                                             <  2:527220/140‑1 (MQ=255)    gTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGAt                                                                                                                             <  2:446386/140‑1 (MQ=255)     ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATc                                                                                                                            >  2:872315/1‑140 (MQ=255)     ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTCGGTGCCGTCGAAACCCTGACGCCACTGATc                                                                                                                            >  2:437056/1‑140 (MQ=255)      tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTTCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGAt                                                                                                                             >  2:399577/1‑138 (MQ=255)      tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTTCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGAt                                                                                                                             <  1:399577/138‑1 (MQ=255)         tcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCt                                                                                                                        >  2:556026/1‑140 (MQ=255)         tcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCt                                                                                                                        >  2:1219953/1‑140 (MQ=255)          cttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTCTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTg                                                                                                                       <  2:1255542/140‑1 (MQ=255)             gCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTgggg                                                                                                                    <  1:556026/140‑1 (MQ=255)             gCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTgggg                                                                                                                    <  2:427375/140‑1 (MQ=255)              cGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGa                                                                                                                   <  1:872315/140‑1 (MQ=255)              cGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTCGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGa                                                                                                                   <  1:437056/140‑1 (MQ=255)               gTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGaa                                                                                                                  >  2:689624/1‑140 (MQ=255)                  tGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATgg                                                                                                               <  1:297192/140‑1 (MQ=255)                  tGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATgg                                                                                                               <  2:1423931/140‑1 (MQ=255)                  tGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATgg                                                                                                               <  1:1249922/140‑1 (MQ=255)                    gTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGc                                                                                                             >  1:432344/1‑140 (MQ=255)                       tGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATg                                                                                                          >  1:438997/1‑140 (MQ=255)                       tGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACAGATCGGCTGGGGAATGGGCATg                                                                                                          >  1:1011517/1‑140 (MQ=255)                        gTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGt                                                                                                         >  2:1294842/1‑140 (MQ=255)                                   cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCgg                                                                                              >  2:307013/1‑140 (MQ=255)                                   cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCgg                                                                                              >  2:1172833/1‑140 (MQ=255)                                          tgcATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTcc                                                                                       <  1:1294842/140‑1 (MQ=255)                                           gcATCAATCGGTAAAGGTGCCACCCTCCATAAATCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCt                                                                                      <  2:1353766/140‑1 (MQ=255)                                           gcATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCt                                                                                      <  2:575670/140‑1 (MQ=255)                                             aTCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCTTTg                                                                                    >  1:1237047/1‑140 (MQ=255)                                             aTCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTg                                                                                    >  1:666604/1‑140 (MQ=255)                                                  tCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATgg                                                                               <  2:432344/140‑1 (MQ=255)                                                   cGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGa                                                                              >  2:1401516/1‑140 (MQ=255)                                                   cGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGa                                                                              >  2:412778/1‑140 (MQ=255)                                                      tAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAAcc                                                                           >  2:922612/1‑140 (MQ=255)                                                      tAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAAcc                                                                           >  2:1378512/1‑140 (MQ=255)                                                      tAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAAcc                                                                           <  1:359203/140‑1 (MQ=255)                                                        aaGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCAc                                                                         <  1:1401516/140‑1 (MQ=255)                                                        aaGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCAc                                                                         <  1:412778/140‑1 (MQ=255)                                                            tGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTcc                                                                                       <  1:1353391/122‑1 (MQ=255)                                                            tGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTcc                                                                                       >  2:1353391/1‑122 (MQ=255)                                                            tGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGa                                                                     >  2:8688/1‑140 (MQ=255)                                                            tGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGa                                                                     >  1:444723/1‑140 (MQ=255)                                                            tGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGa                                                                     >  2:645329/1‑140 (MQ=255)                                                             gCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAt                                                                    <  2:625212/140‑1 (MQ=255)                                                              ccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAtt                                                                   >  1:559716/1‑140 (MQ=255)                                                              ccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAtt                                                                   >  1:1232592/1‑140 (MQ=255)                                                              ccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAtt                                                                   >  2:529439/1‑140 (MQ=255)                                                                 cccTCCATAAACCGAAATTTTCTGAAGCATCGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCg                                                                <  2:178218/140‑1 (MQ=255)                                                                     ccATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtg                                                            >  2:377539/1‑140 (MQ=255)                                                                      cATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCTTTGAATGGAACCACTGGATTGCGTTtgt                                                           <  2:1237047/140‑1 (MQ=255)                                                                      cATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtgt                                                           >  1:1190675/1‑140 (MQ=255)                                                                      cATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtgt                                                           >  1:219827/1‑140 (MQ=255)                                                                      cATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtgt                                                           >  2:397932/1‑140 (MQ=255)                                                                        tAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgc                                                         <  2:219827/140‑1 (MQ=255)                                                                        tAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgc                                                         <  1:1378512/140‑1 (MQ=255)                                                                         aaaCCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgct                                                        <  1:768025/140‑1 (MQ=255)                                                                               aaaTTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat                                                  >  2:68805/1‑140 (MQ=255)                                                                                aaTTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCCCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGt                                                               <  2:106846/126‑1 (MQ=255)                                                                                aaTTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGt                                                               >  1:106846/1‑126 (MQ=255)                                                                                aaTTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGt                                                               <  2:119729/126‑1 (MQ=255)                                                                                aaTTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGt                                                               >  1:119729/1‑126 (MQ=255)                                                                                aaTTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtg                                                            <  1:535326/129‑1 (MQ=255)                                                                                aaTTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtg                                                            >  2:535326/1‑129 (MQ=255)                                                                                       tGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTcg                                          <  2:511260/140‑1 (MQ=255)                                                                                          aGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTcggcg                                       <  1:645329/140‑1 (MQ=255)                                                                                              ttGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCggcgggcg                                   <  1:397932/140‑1 (MQ=255)                                                                                                gCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGaa                                 <  2:559716/140‑1 (MQ=255)                                                                                                   aaCCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCggcgggcg                                   >  2:39172/1‑135 (MQ=255)                                                                                                   aaCCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCggcgggcg                                   <  1:39172/135‑1 (MQ=255)                                                                                                        gCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGattatt                         >  1:391673/1‑140 (MQ=255)                                                                                                         ccTTATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTg                        >  1:504041/1‑140 (MQ=255)                                                                                                            tATTTTTGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAgg                     >  2:5960/1‑140 (MQ=255)                                                                                                              tttttGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGt                   >  1:10421/1‑140 (MQ=255)                                                                                                                 ttGGTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGtttt                <  1:1364476/140‑1 (MQ=255)                                                                                                                   ggTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCg              <  1:306162/140‑1 (MQ=255)                                                                                                                   ggTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCg              <  2:391673/140‑1 (MQ=255)                                                                                                                    gTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCg              >  2:252639/1‑139 (MQ=255)                                                                                                                    gTGCCGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCg              <  1:252639/139‑1 (MQ=255)                                                                                                                        cGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGgcg         >  2:479136/1‑140 (MQ=255)                                                                                                                        cGTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGgcg         <  2:10421/140‑1 (MQ=255)                                                                                                                         gTCGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGgcgc        <  2:488920/140‑1 (MQ=255)                                                                                                                            gAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAga     <  1:68805/140‑1 (MQ=255)                                                                                                                             aaaCCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAgat    <  1:5960/140‑1 (MQ=255)                                                                                                                               aCCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAgatga  <  2:783918/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 ACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGA  >  NC_000913/1905703‑1905968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑