Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	199,082	T→C	G385G (GGT→GGC)	bamA →	outer membrane protein assembly factor BamA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	199,082	0	T	C	100.0%	292.8 / NA	81	G385G (GGT→GGC)	bamA	outer membrane protein assembly factor BamA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/42);  total (39/42)

CGTTGATGCGGGTAACCGTTTCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTAT  >  NC_000913/198947‑199216                                                                                                                                        |                                                                                                                                       cGTTGATGCGGGTAACCGTTTCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAgg                                                                                                                                    <  2:399679/140‑1 (MQ=255)   ttGATGCGGGTAACCGTTTCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAg                                                                                                                                  >  2:1117540/1‑140 (MQ=255)   ttGATGCGGGTAACCGTTTCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAg                                                                                                                                  <  1:1160103/140‑1 (MQ=255)    tGATGCGGGTAACCGTTTCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGc                                                                                                                                 >  2:1416823/1‑140 (MQ=255)      aTGCGGGTAACCGTTTCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGt                                                                                                                               <  1:1416823/140‑1 (MQ=255)            gTAACCGTTTCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAAt                                                                                                                         >  2:777385/1‑140 (MQ=255)                 cGTTTCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCt                                                                                                                    >  2:830676/1‑140 (MQ=255)                     tCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCGTGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGc                                                                                                                <  2:81136/140‑1 (MQ=255)                     tCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGc                                                                                                                <  1:1026579/140‑1 (MQ=255)                     tCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGc                                                                                                                <  1:772470/140‑1 (MQ=255)                     tCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGc                                                                                                                <  1:692570/140‑1 (MQ=255)                     tCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGc                                                                                                                <  1:576062/140‑1 (MQ=255)                           tGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCttt                                                                                                          >  2:111250/1‑140 (MQ=255)                           tGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCttt                                                                                                          >  2:859880/1‑140 (MQ=255)                              gTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGaa                                                                                                       <  1:111250/140‑1 (MQ=255)                               tAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGaaa                                                                                                      >  2:543676/1‑140 (MQ=255)                                     ccGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcg                                                                                                >  1:1092292/1‑140 (MQ=255)                                      cGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcga                                                                                               >  1:1165804/1‑140 (MQ=255)                                      cGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcga                                                                                               <  2:722584/140‑1 (MQ=255)                                      cGTTTTGAAGGTAACGATACCTCGAAAGATGCAGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcga                                                                                               <  1:19379/140‑1 (MQ=255)                                        ttttGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgata                                                                                             >  1:989632/1‑140 (MQ=255)                                        ttttGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgata                                                                                             >  2:620764/1‑140 (MQ=255)                                        ttttGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgata                                                                                             <  1:1177893/140‑1 (MQ=255)                                        ttttGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgata                                                                                             >  1:737828/1‑140 (MQ=255)                                        ttttGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgata                                                                                             >  2:460678/1‑140 (MQ=255)                                             aaGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTC‑TTGAAACTGTcgataccgata                                                                                       >  1:726811/1‑140 (MQ=255)                                              aGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTC‑TTGAAACTGTcgataccgatac                                                                                      <  2:726811/140‑1 (MQ=255)                                               ggTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGaa                                                                                                       >  2:166645/1‑123 (MQ=255)                                               ggTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGaa                                                                                                       <  1:166645/123‑1 (MQ=255)                                               ggTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgataccgatac                                                                                      <  2:201184/140‑1 (MQ=255)                                                  aaCGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCa                                                                                   >  1:738024/1‑140 (MQ=255)                                                   aCGATACCTCGATAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGC‑GGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAAc                                                                                 >  2:165829/1‑140 (MQ=255)                                                   aCGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCaa                                                                                  >  2:1089726/1‑140 (MQ=255)                                                    cGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAAc                                                                                 >  2:1330613/1‑140 (MQ=255)                                                    cGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAAc                                                                                 <  2:738024/140‑1 (MQ=255)                                                    cGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAAc                                                                                 <  1:460678/140‑1 (MQ=255)                                                         ccTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGtt                                                                            <  1:221791/140‑1 (MQ=255)                                                          cTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTc                                                                           >  2:1417647/1‑140 (MQ=255)                                                             gAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCgg                                                                        >  1:1120532/1‑140 (MQ=255)                                                              aaaGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCggg                                                                       >  1:1277322/1‑140 (MQ=255)                                                                  aTGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGc                                                                   <  1:1231455/140‑1 (MQ=255)                                                                   tGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGcc                                                                  >  1:757304/1‑140 (MQ=255)                                                                   tGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGcc                                                                  <  2:1165804/140‑1 (MQ=255)                                                                   tGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGcc                                                                  >  1:336589/1‑140 (MQ=255)                                                                     ccGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCg                                                                <  1:1417647/140‑1 (MQ=255)                                                                       gTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGC‑GGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGAc                                                             <  1:165829/140‑1 (MQ=255)                                                                        tCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGAc                                                             <  1:1117540/140‑1 (MQ=255)                                                                        tCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGAc                                                             <  2:989632/140‑1 (MQ=255)                                                                            gCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAgg                                                         <  2:1092292/140‑1 (MQ=255)                                                                             cGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGt                                                        >  2:252829/1‑140 (MQ=255)                                                                               tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg                                                      >  2:525957/1‑140 (MQ=255)                                                                               tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg                                                      <  1:830676/140‑1 (MQ=255)                                                                               tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg                                                      <  2:832573/140‑1 (MQ=255)                                                                                cgcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGa                                                     <  2:757304/140‑1 (MQ=255)                                                                                 gcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATTAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGAt                                                    <  2:1277322/140‑1 (MQ=255)                                                                                  cgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATg                                                   >  1:737205/1‑140 (MQ=255)                                                                                  cgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATg                                                   <  1:1330613/140‑1 (MQ=255)                                                                                  cgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATg                                                   >  2:253660/1‑140 (MQ=255)                                                                                  cgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATg                                                   >  2:258188/1‑140 (MQ=255)                                                                                   gAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATgt                                                  <  2:336589/140‑1 (MQ=255)                                                                                         cGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTa                                            <  1:649444/140‑1 (MQ=255)                                                                                         cGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTa                                            <  1:1123044/140‑1 (MQ=255)                                                                                                   aaGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTaaaa                                  <  1:1089726/140‑1 (MQ=255)                                                                                                        gCATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgcg                             <  1:116083/140‑1 (MQ=255)                                                                                                         cATGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgcgc                            >  1:451684/1‑140 (MQ=255)                                                                                                           tGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTa                                            <  1:780592/122‑1 (MQ=255)                                                                                                           tGGCTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTa                                            >  2:780592/1‑122 (MQ=255)                                                                                                              cTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAacac                       >  1:970703/1‑140 (MQ=255)                                                                                                              cTGGGGAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAacac                       >  1:660932/1‑140 (MQ=255)                                                                                                                 gggAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCgg                    >  2:124249/1‑140 (MQ=255)                                                                                                                 gggAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCgg                    <  2:1072461/140‑1 (MQ=255)                                                                                                                  ggAGCGATCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGt                   <  2:737828/140‑1 (MQ=255)                                                                                                                        aTCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTc             >  2:395105/1‑140 (MQ=255)                                                                                                                        aTCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTc             >  2:544583/1‑140 (MQ=255)                                                                                                                        aTCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTc             >  1:862393/1‑140 (MQ=255)                                                                                                                        aTCTGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTc             <  1:543676/140‑1 (MQ=255)                                                                                                                           tGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAAc          >  1:687179/1‑140 (MQ=255)                                                                                                                           tGGTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAAc          <  1:620764/140‑1 (MQ=255)                                                                                                                            ggTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACt         <  2:207690/140‑1 (MQ=255)                                                                                                                             gTCGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACtt        <  1:253660/140‑1 (MQ=255)                                                                                                                               cGATCAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTg      <  1:1157543/140‑1 (MQ=255)                                                                                                                                   cAGGGCAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTAt  >  1:81369/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CGTTGATGCGGGTAACCGTTTCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTAT  >  NC_000913/198947‑199216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑