Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,058	T→C	S114S (AGT→AGC)	yeiH →	conserved inner membrane protein YeiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,058	0	T	C	100.0%	287.8 / NA	83	S114S (AGT→AGC)	yeiH	conserved inner membrane protein YeiH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/45);  total (38/45)

GTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAG  >  NC_000913/2249922‑2250194                                                                                                                                         |                                                                                                                                         gtgACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCAcc                                                                                                                                       >  2:1247111/1‑140 (MQ=255)         ggtgTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCctgct                                                                                                                               <  1:749196/140‑1 (MQ=255)         ggtgTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCctgct                                                                                                                               >  1:1166645/1‑140 (MQ=255)            gtgCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGgc                                                                                                                            <  2:928281/140‑1 (MQ=255)             tgCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGgct                                                                                                                           <  1:980649/140‑1 (MQ=255)               cTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCt                                                                                                                                      >  2:959223/1‑127 (MQ=255)               cTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCt                                                                                                                                      <  1:959223/127‑1 (MQ=255)               cTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGgcttg                                                                                                                         <  1:655946/140‑1 (MQ=255)               cTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGgcttg                                                                                                                         <  1:1247111/140‑1 (MQ=255)               cTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTACCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGgcttg                                                                                                                         <  1:539699/140‑1 (MQ=255)                    tGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTcc                                                                                                                    >  2:2043/1‑140 (MQ=255)                    tGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTcc                                                                                                                    >  2:328383/1‑140 (MQ=255)                     gCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCt                                                                                                                   >  1:1323974/1‑140 (MQ=255)                     gCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCt                                                                                                                   >  2:585865/1‑140 (MQ=255)                         aGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTgggg                                                                                                               >  2:609059/1‑140 (MQ=255)                          gCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGc                                                                                                              >  2:1025137/1‑140 (MQ=255)                                   attaCGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAgtgt                                                                                                     <  1:1309575/140‑1 (MQ=255)                                   attaCGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAgtgt                                                                                                     <  1:1191282/140‑1 (MQ=255)                                    ttaCGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGtt                                                                                                    <  2:312941/140‑1 (MQ=255)                                    ttaCGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGtt                                                                                                    <  1:641375/140‑1 (MQ=255)                                       cGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTgg                                                                                                 <  1:90619/140‑1 (MQ=255)                                       cGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTgg                                                                                                 <  1:948092/140‑1 (MQ=255)                                        gTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGt                                                                                                >  2:940906/1‑140 (MQ=255)                                        gTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGt                                                                                                >  2:246004/1‑140 (MQ=255)                                        gTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGt                                                                                                >  2:252732/1‑140 (MQ=255)                                         tCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGt                                                                                                >  2:101068/1‑139 (MQ=255)                                         tCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGt                                                                                                <  1:101068/139‑1 (MQ=255)                                         tCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTc                                                                                               >  2:191848/1‑140 (MQ=255)                                         tCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTc                                                                                               >  2:1084319/1‑140 (MQ=255)                                          cTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTc                                                                                               <  2:919078/139‑1 (MQ=255)                                          cTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTc                                                                                               >  1:919078/1‑139 (MQ=255)                                            gggTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTgg                                                                                            >  1:21961/1‑140 (MQ=255)                                              gTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGAt                                                                                          <  2:752220/140‑1 (MQ=255)                                               tATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATa                                                                                         <  2:529324/140‑1 (MQ=255)                                                aTCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATaa                                                                                        <  1:940906/140‑1 (MQ=255)                                                 tCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAg                                                                                       <  1:328383/140‑1 (MQ=255)                                                   aTTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGca                                                                                     >  2:65467/1‑140 (MQ=255)                                                    ttCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGcac                                                                                    >  1:800397/1‑140 (MQ=255)                                                       tttATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACAcc                                                                                 >  2:386607/1‑140 (MQ=255)                                                        ttATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCa                                                                                <  1:191848/140‑1 (MQ=255)                                                          aTGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGc                                                                              <  1:895024/140‑1 (MQ=255)                                                              cTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGt                                                                          >  2:102137/1‑140 (MQ=255)                                                                tCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTg                                                                        >  2:340011/1‑140 (MQ=255)                                                                 ccGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGa                                                                       <  1:864561/140‑1 (MQ=255)                                                                 ccGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGa                                                                       >  1:1195722/1‑140 (MQ=255)                                                                  cGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGAt                                                                      <  1:585865/140‑1 (MQ=255)                                                                  cGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGAt                                                                      >  2:546523/1‑140 (MQ=255)                                                                      tGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGCTCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGt                                                                  <  2:1195722/140‑1 (MQ=255)                                                                      tGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGt                                                                  <  1:252732/140‑1 (MQ=255)                                                                      tGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGt                                                                  <  1:246004/140‑1 (MQ=255)                                                                      tGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGt                                                                  >  1:270680/1‑140 (MQ=255)                                                                      tGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGt                                                                  <  2:1001062/140‑1 (MQ=255)                                                                           ttCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCgg                                                             <  1:65467/140‑1 (MQ=255)                                                                            tctcGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGt                                                            <  2:270680/140‑1 (MQ=255)                                                                             ctcGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTa                                                           >  1:1039946/1‑140 (MQ=255)                                                                               cGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGc                                                         <  2:21961/140‑1 (MQ=255)                                                                               cGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGc                                                         <  2:192265/140‑1 (MQ=255)                                                                                  aaaTTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCg                                                              <  1:876957/132‑1 (MQ=255)                                                                                  aaaTTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGt                                                      <  1:1025137/140‑1 (MQ=255)                                                                                  aaaTTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCAGTGCCg                                                              >  2:876957/1‑132 (MQ=255)                                                                                       gCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCtg                                                 <  2:648672/140‑1 (MQ=255)                                                                                             gTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGtgc                                           <  2:1352814/140‑1 (MQ=255)                                                                                                 gTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGcc                                       >  2:493927/1‑140 (MQ=255)                                                                                                       gTGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTa                                 <  1:2043/140‑1 (MQ=255)                                                                                                        tGGGATCATCATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTAc                                >  1:348402/1‑140 (MQ=255)                                                                                                            atcatcATTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGc                            >  2:1208450/1‑140 (MQ=255)                                                                                                               atcatTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGctac                         <  2:1166645/140‑4 (MQ=255)                                                                                                               atcatTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGAc                         >  2:1041038/1‑140 (MQ=255)                                                                                                               atcatTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGAc                         <  1:343264/140‑1 (MQ=255)                                                                                                               atcatTGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGAc                         <  1:1041038/140‑1 (MQ=255)                                                                                                                    tGACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGc                    >  2:1007189/1‑140 (MQ=255)                                                                                                                     gACGTGTTGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGcc                   <  2:746235/140‑1 (MQ=255)                                                                                                                           ttGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGt             >  1:113238/1‑140 (MQ=255)                                                                                                                           ttGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGt             >  2:81179/1‑140 (MQ=255)                                                                                                                           ttGACGCTGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGt             >  1:299339/1‑140 (MQ=255)                                                                                                                                  tGTCCGGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCg      <  1:1351560/140‑1 (MQ=255)                                                                                                                                  tGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGGTCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCg      >  1:923796/1‑140 (MQ=255)                                                                                                                                  tGTCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCg      >  1:1103049/1‑140 (MQ=255)                                                                                                                                    tCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGa    >  2:234969/1‑140 (MQ=255)                                                                                                                                    tCCAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGa    <  1:493927/140‑1 (MQ=255)                                                                                                                                     ccAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCTGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGaa   <  1:899977/140‑1 (MQ=255)                                                                                                                                     ccAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGATAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGaa   <  1:1084319/140‑1 (MQ=255)                                                                                                                                      cAGCACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAg  <  1:386607/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAG  >  NC_000913/2249922‑2250194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑