Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,291,549	T→C	T288A (ACG→GCG)	ccmH ←	holocytochrome c synthetase ‑ thiol:disulfide oxidoreductase CcmH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,291,549	0	T	C	100.0%	329.1 / NA	93	T288A (ACG→GCG)	ccmH	holocytochrome c synthetase ‑ thiol:disulfide oxidoreductase CcmH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/50);  total (43/50)

AATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGTTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGCGTATGCA  >  NC_000913/2291412‑2291682                                                                                                                                          |                                                                                                                                      aaTCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCtc                                                                                                                                     >  1:616798/1‑140 (MQ=255) aaTCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCtc                                                                                                                                     >  2:765500/1‑140 (MQ=255) aaTCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCtc                                                                                                                                     >  2:556638/1‑140 (MQ=255) aaTCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCtc                                                                                                                                     >  2:540485/1‑140 (MQ=255) aaTCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCtc                                                                                                                                     >  2:1383751/1‑140 (MQ=255)   tCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCtctc                                                                                                                                   >  1:781487/1‑140 (MQ=255)    cACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCa                                                                                                                                  >  2:1202463/1‑140 (MQ=255)      ccGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCAcc                                                                                                                                >  1:1230718/1‑140 (MQ=255)      ccGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCAcc                                                                                                                                >  1:125898/1‑140 (MQ=255)      ccGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCAcc                                                                                                                                >  2:1147275/1‑140 (MQ=255)        gCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAg                                                                                                                              <  2:616798/140‑1 (MQ=255)         cACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGc                                                                                                                             <  2:1255652/140‑1 (MQ=255)         cACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGc                                                                                                                             >  2:1221333/1‑140 (MQ=255)            ggCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGa                                                                                                                          <  1:540485/140‑1 (MQ=255)            ggCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGa                                                                                                                          <  2:897286/140‑1 (MQ=255)              cGAGTATCGTTGGCAGGTAAGGGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACg                                                                                                                        <  2:1030964/140‑1 (MQ=255)                 gTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAg                                                                                                                     <  2:102610/140‑1 (MQ=255)                 gTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAg                                                                                                                     <  1:765500/140‑1 (MQ=255)                 gTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAg                                                                                                                     >  2:986669/1‑140 (MQ=255)                 gTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAg                                                                                                                     <  1:386151/140‑1 (MQ=255)                       ttGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTc                                                                                                               >  1:501294/1‑140 (MQ=255)                        tGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCa                                                                                                              >  2:810586/1‑140 (MQ=255)                          gCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCAcc                                                                                                            >  1:263030/1‑140 (MQ=255)                              gTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCAccgccg                                                                                                        >  1:908545/1‑140 (MQ=255)                                 agagTTTCAACATCATCTCCCACGCGGCAACGGTTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACCCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAgg                                                                                                     <  1:694220/140‑1 (MQ=255)                                     tttCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGt                                                                                                 <  2:501294/140‑1 (MQ=255)                                     tttCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGt                                                                                                 >  1:96354/1‑140 (MQ=255)                                      ttCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGtt                                                                                                <  1:810586/140‑1 (MQ=255)                                       tCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTg                                                                                               <  2:395478/140‑1 (MQ=255)                                       tCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTg                                                                                               <  2:752066/140‑1 (MQ=255)                                            atcatcTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGtt                                                                                          <  2:362113/140‑1 (MQ=255)                                            atcatcTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGtt                                                                                          <  2:1293787/140‑1 (MQ=255)                                               atcTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTggg                                                                                       <  2:1227748/140‑1 (MQ=255)                                                tctcCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGa                                                                                      >  2:932904/1‑140 (MQ=255)                                                      aCGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGAt                                                                                >  2:1367920/1‑140 (MQ=255)                                                       cgcgGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATg                                                                               >  2:835699/1‑140 (MQ=255)                                                        gcgGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGa                                                                              >  2:330799/1‑140 (MQ=255)                                                         cgGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGGTGTCCTTGGGAACAGGTgga                                                                             >  1:408538/1‑138 (MQ=255)                                                          ggCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAAc                                                                            >  1:1042950/1‑140 (MQ=255)                                                           gCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACg                                                                           >  2:216500/1‑140 (MQ=255)                                                            cAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTTGGGTCAGATTAACGa                                                                          >  2:1079241/1‑140 (MQ=255)                                                            cAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGa                                                                          >  2:832309/1‑140 (MQ=255)                                                            cAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGa                                                                          >  1:1381670/1‑140 (MQ=255)                                                               cGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCTCACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTc                                                                       <  2:1243133/140‑1 (MQ=255)                                                                ggCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCa                                                                      <  2:472882/140‑1 (MQ=255)                                                                ggCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCa                                                                      <  1:1367920/140‑1 (MQ=255)                                                                     cGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCt                                                                 <  2:781487/140‑1 (MQ=255)                                                                      gCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCtt                                                                <  1:90212/140‑1 (MQ=255)                                                                      gCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCtt                                                                <  1:1202463/140‑1 (MQ=255)                                                                      gCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCtt                                                                <  1:216500/140‑1 (MQ=255)                                                                      gCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCtt                                                                <  1:832309/140‑1 (MQ=255)                                                                      gCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCtt                                                                <  1:1221333/140‑1 (MQ=255)                                                                      gCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCtt                                                                <  1:1179809/140‑1 (MQ=255)                                                                           aTCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCg                                                           <  2:263030/140‑1 (MQ=255)                                                                           aTCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCg                                                           <  1:835699/140‑1 (MQ=255)                                                                              gctgctgctCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTAt                                                        <  1:556638/140‑1 (MQ=255)                                                                                tgctgctCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATcc                                                      <  1:958215/140‑1 (MQ=255)                                                                                tgctgctCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATcc                                                      <  2:1381670/140‑1 (MQ=255)                                                                                        aaaGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTgcag                                              <  2:908545/140‑1 (MQ=255)                                                                                           ggCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCAt                                           <  2:1179514/140‑1 (MQ=255)                                                                                             cATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCa                                         >  2:1043208/1‑140 (MQ=255)                                                                                               ttAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACt                                       <  1:1125054/140‑1 (MQ=255)                                                                                                  aaCGCATACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTTGGGTCAGATGAACGAGTCAACGCCTCCGCGGATCCCCGTGCAGCATCACTGtt                                    >  2:703118/1‑140 (MQ=255)                                                                                                       aTACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCg                               >  1:850392/1‑140 (MQ=255)                                                                                                       aTACATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCg                               >  1:485490/1‑140 (MQ=255)                                                                                                          cATGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGAt                            >  2:801649/1‑140 (MQ=255)                                                                                                            tGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCg                          <  1:801649/140‑1 (MQ=255)                                                                                                            tGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCg                          <  2:1230718/140‑1 (MQ=255)                                                                                                            tGCTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCg                          <  1:703118/140‑1 (MQ=255)                                                                                                              cTTAGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAg                        <  1:932904/140‑1 (MQ=255)                                                                                                                 aGCACACGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCg                     >  2:277841/1‑140 (MQ=255)                                                                                                                   cacaCGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGAt                   <  1:330799/140‑1 (MQ=255)                                                                                                                      acGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACg                <  1:986669/140‑1 (MQ=255)                                                                                                                      acGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACg                <  2:850392/140‑1 (MQ=255)                                                                                                                      acGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACg                <  2:485490/140‑1 (MQ=255)                                                                                                                       cGGATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGc               >  1:1028020/1‑140 (MQ=255)                                                                                                                        ggATATTGTTATGGTCCCCTCTCACCAGCTGACGTAGTAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCa              <  2:1037092/140‑1 (MQ=255)                                                                                                                        ggATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCa              <  1:116640/140‑1 (MQ=255)                                                                                                                        ggATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCa              >  2:116640/1‑140 (MQ=255)                                                                                                                        ggATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCa              <  1:1358384/140‑1 (MQ=255)                                                                                                                        ggATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCa              <  2:1042950/140‑1 (MQ=255)                                                                                                                        ggATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCa              <  1:94419/140‑1 (MQ=255)                                                                                                                        ggATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCa              <  1:917860/140‑1 (MQ=255)                                                                                                                         gATATTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAg             <  1:1383751/140‑1 (MQ=255)                                                                                                                          atatTGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGt            <  2:125898/140‑1 (MQ=255)                                                                                                                             ttGCTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGc         >  2:133672/1‑140 (MQ=255)                                                                                                                                cTATGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGCGTa      <  1:1079241/140‑1 (MQ=255)                                                                                                                                  aTGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGCGTATg    >  1:729463/1‑140 (MQ=255)                                                                                                                                   tGGTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGCGTATGc   >  1:1352488/1‑140 (MQ=255)                                                                                                                                    ggTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGCGTATGCa  >  1:617811/1‑140 (MQ=255)                                                                                                                                    ggTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGCGTATGCa  >  2:3405/1‑140 (MQ=255)                                                                                                                                    ggTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGCGTATGCa  >  1:711000/1‑140 (MQ=255)                                                                                                                                    ggTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGCGTATGCa  >  1:1222878/1‑140 (MQ=255)                                                                                                                                    ggTCCGCTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGCGTATGCa  >  2:23836/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      AATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAATCGCTGCTGCTCAAAGGCATTAAACGCATACATGCTTAGCACACGGATATTGCTATGGTCCGTTCTCACCAGCTGACGTAGCAGTTCACCGCCGAGGCGGTTGTCGTTGGGATCAGATGAACGAGTCAACGCTTCAGCGTATCCCAGTGCAGCATCACTGTTTTTCGGATCGAGGCGATACGCAGTGGCGTATGCA  >  NC_000913/2291412‑2291682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑