Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,326,867	T→C	G253G (GGT→GGC)	atoB →	acetyl‑CoA acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,326,867	0	T	C	100.0%	161.6 / NA	47	G253G (GGT→GGC)	atoB	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/23);  total (24/23)

CGAAAGAAAACCTTCGTCTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAA  >  NC_000913/2326730‑2327003                                                                                                                                          |                                                                                                                                         cGAAAGAAAACCTTCGTCTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGgcgc                                                                                                                                        <  2:168296/140‑1 (MQ=255)   aaagaaaACCTTCGTCTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACTGCTGGGAACGCGTCTGGTATTAACGACGGcgctg                                                                                                                                      <  1:62113/140‑1 (MQ=255)        aaaCCTTCGTCTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGct                                                                                                                                 <  2:1155845/140‑1 (MQ=255)              tCGTCTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTg                                                                                                                           >  2:492499/1‑140 (MQ=255)               cGTCTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGa                                                                                                                          <  2:260806/140‑1 (MQ=255)                                aaTTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTgcg                                                                                                         <  1:925494/140‑1 (MQ=255)                                  ttCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTgcggc                                                                                                       >  2:1204416/1‑140 (MQ=255)                                    cccGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGgcgc                                                                                                     >  1:472012/1‑140 (MQ=255)                                          aGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGgcag                                                                                               <  2:1360137/140‑1 (MQ=255)                                           gCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGgcagc                                                                                              <  1:1013395/140‑1 (MQ=255)                                            cGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGgcagca                                                                                             <  2:1172190/140‑1 (MQ=255)                                                     cGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGgcag                                                                                               >  2:594944/1‑129 (MQ=255)                                                     cGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGgcag                                                                                               <  1:594944/129‑1 (MQ=255)                                                     cGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTAcc                                                                                    >  1:1686/1‑140 (MQ=255)                                                           aaGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTg                                                                              >  2:1018723/1‑140 (MQ=255)                                                              cGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCTCCTGGCt                                                                           <  1:1204416/140‑1 (MQ=255)                                                                ttAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCg                                                                         >  1:765380/1‑140 (MQ=255)                                                                          tGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGt                                                               >  2:1239485/1‑140 (MQ=255)                                                                           gcgcCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGtt                                                              <  1:843441/140‑1 (MQ=255)                                                                               ccGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGc                                                          >  2:1411343/1‑140 (MQ=255)                                                                                 ggCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGc                                                          >  2:1379733/1‑138 (MQ=255)                                                                                 ggCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGc                                                          <  1:107509/138‑1 (MQ=255)                                                                                 ggCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGc                                                          >  2:107509/1‑138 (MQ=255)                                                                                 ggCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGc                                                          <  1:1379733/138‑1 (MQ=255)                                                                                 ggCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCa                                                        >  1:130532/1‑140 (MQ=255)                                                                                 ggCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCa                                                        >  1:509169/1‑140 (MQ=255)                                                                                  gCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAg                                                       <  2:472012/140‑1 (MQ=255)                                                                                    cTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCg                                                     <  2:765380/140‑1 (MQ=255)                                                                                       cGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTg                                                  >  1:334501/1‑140 (MQ=255)                                                                                             aGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGc                                            >  1:75575/1‑140 (MQ=255)                                                                                                  gAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGcccccc                                       >  1:1369724/1‑140 (MQ=255)                                                                                                     cAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCa                                    <  2:56218/140‑1 (MQ=255)                                                                                                        tCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTg                                 >  1:1133699/1‑140 (MQ=255)                                                                                                        tCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTg                                 >  1:1183529/1‑140 (MQ=255)                                                                                                        tCACCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGCTGGCGTGCCCCCCGCATTg                                 >  1:906007/1‑140 (MQ=255)                                                                                                          aCCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGAt                               <  1:1018723/140‑1 (MQ=255)                                                                                                          aCCGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGAt                               <  1:1239485/140‑1 (MQ=255)                                                                                                           ccGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATg                              <  2:509169/140‑1 (MQ=255)                                                                                                           ccGCTGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATg                              <  2:75575/140‑1 (MQ=255)                                                                                                               tGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTa                          >  2:243496/1‑140 (MQ=255)                                                                                                               tGGGAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTa                          <  1:1411343/140‑1 (MQ=255)                                                                                                                  gAACGCGTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATgg                       >  2:16902/1‑140 (MQ=255)                                                                                                                   aaCGCGTCTGGTATTACCGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATggg                      <  2:1280998/140‑1 (MQ=255)                                                                                                                     cgcgTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGc                    <  1:41054/140‑1 (MQ=255)                                                                                                                        gTCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAg                 >  2:650071/1‑140 (MQ=255)                                                                                                                         tCTGGTATTAACGACGGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGt                >  2:593221/1‑140 (MQ=255)                                                                                                                                   acgacgGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACg      >  2:620362/1‑140 (MQ=255)                                                                                                                                       cgGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACACaaa  <  1:492499/140‑1 (MQ=255)                                                                                                                                       cgGCGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATCAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCaaa  <  2:1297769/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CGAAAGAAAACCTTCGTCTTCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAA  >  NC_000913/2326730‑2327003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑