Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,399,580	T→C	N196D (AAC→GAC)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,399,580	0	T	C	100.0%	348.2 / NA	97	N196D (AAC→GAC)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/50);  total (47/50)

GGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCA  >  NC_000913/2399451‑2399715                                                                                                                                  |                                                                                                                                        ggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAAt                                                                                                                               >  1:200504/1‑140 (MQ=255) ggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAAt                                                                                                                               >  1:1364055/1‑140 (MQ=255) ggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAAt                                                                                                                               >  2:566296/1‑140 (MQ=255)      aTGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCt                                                                                                                          >  2:1374491/1‑140 (MQ=255)           aCAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCa                                                                                                                     >  1:500221/1‑140 (MQ=255)           aCAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCa                                                                                                                     >  1:470256/1‑140 (MQ=255)           aCAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCa                                                                                                                     >  2:876766/1‑140 (MQ=255)                cGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTg                                                                                                                >  1:601558/1‑140 (MQ=255)                cGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATAACCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTg                                                                                                                >  2:991970/1‑140 (MQ=255)                 gATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGc                                                                                                               >  1:519235/1‑140 (MQ=255)                 gATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGc                                                                                                               <  2:1364055/140‑1 (MQ=255)                  aTGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGcc                                                                                                              >  1:1229870/1‑140 (MQ=255)                  aTGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGcc                                                                                                              >  2:813193/1‑140 (MQ=255)                  aTGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGcc                                                                                                              >  1:1418875/1‑140 (MQ=255)                   tGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCg                                                                                                                            <  2:713971/125‑1 (MQ=255)                   tGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCg                                                                                                                            >  1:713971/1‑125 (MQ=255)                   tGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCg                                                                                                             >  2:1152713/1‑140 (MQ=255)                   tGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCg                                                                                                             <  1:956471/140‑1 (MQ=255)                   tGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCg                                                                                                             >  2:956471/1‑140 (MQ=255)                   tGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCg                                                                                                             >  1:1200272/1‑140 (MQ=255)                          ttGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCg                                                                                                      >  1:1251948/1‑140 (MQ=255)                             cTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGc                                                                                                   >  1:1347964/1‑140 (MQ=255)                             cTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGc                                                                                                   <  1:1165502/140‑1 (MQ=255)                               ggCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGa                                                                                                 <  1:125720/140‑1 (MQ=255)                               ggCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGa                                                                                                 <  2:1347964/140‑1 (MQ=255)                                gCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGAc                                                                                                <  1:813193/140‑1 (MQ=255)                                gCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGAc                                                                                                <  2:60858/140‑1 (MQ=255)                                gCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGAc                                                                                                <  1:584973/140‑1 (MQ=255)                                gCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGAc                                                                                                <  1:311158/140‑1 (MQ=255)                                gCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGAc                                                                                                <  1:791727/140‑1 (MQ=255)                                gCAGATGCTCGGCGCAAACCGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGAc                                                                                                <  1:930133/140‑1 (MQ=255)                                 cAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCAGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGAcc                                                                                               <  2:470256/140‑1 (MQ=255)                                  aGATTCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCg                                                                                              >  1:1332602/1‑140 (MQ=255)                                  aGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCg                                                                                              >  1:551208/1‑140 (MQ=255)                                    aTGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGaa                                                                                            <  1:566296/140‑1 (MQ=255)                                     tGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAg                                                                                           >  2:101341/1‑140 (MQ=255)                                      gCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGt                                                                                          <  2:1418875/140‑1 (MQ=255)                                       cTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTa                                                                                         >  2:913145/1‑140 (MQ=255)                                       cTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTa                                                                                         >  1:206434/1‑140 (MQ=255)                                           gcgcAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACg                                                                                     <  2:21689/140‑1 (MQ=255)                                             gcAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGtt                                                                                   <  2:316998/140‑1 (MQ=255)                                                     cATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTgcg                                                                           <  2:1229870/140‑1 (MQ=255)                                                             aTTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATATGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTCCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTaa                                                                   <  2:1332602/140‑1 (MQ=255)                                                             aTTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTaa                                                                   <  2:1251948/140‑1 (MQ=255)                                                               ttACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAAc                                                                 >  1:1228410/1‑140 (MQ=255)                                                               ttACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAAc                                                                 >  2:292578/1‑140 (MQ=255)                                                               ttACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAAc                                                                 <  2:551208/140‑1 (MQ=255)                                                               ttACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAAc                                                                 >  1:946860/1‑140 (MQ=255)                                                               ttACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAAc                                                                 >  2:671537/1‑140 (MQ=255)                                                                tACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACg                                                                >  2:318777/1‑140 (MQ=255)                                                                  cGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGcc                                                              >  1:1135980/1‑140 (MQ=255)                                                                  cGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGcc                                                              <  2:200504/140‑1 (MQ=255)                                                                  cGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGcc                                                              <  1:671537/140‑1 (MQ=255)                                                                    gTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCa                                                            <  2:206434/140‑1 (MQ=255)                                                                      tGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTaa                                                                   >  2:217014/1‑131 (MQ=255)                                                                      tGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTaa                                                                   <  1:217014/131‑1 (MQ=255)                                                                      tGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACg                                                                <  2:896310/134‑1 (MQ=255)                                                                      tGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACg                                                                >  1:896310/1‑134 (MQ=255)                                                                        tAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCGAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATc                                                        >  2:1152502/1‑140 (MQ=255)                                                                        tAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATc                                                        >  1:37804/1‑140 (MQ=255)                                                                        tAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATc                                                        >  1:925764/1‑140 (MQ=255)                                                                        tAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCATGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATc                                                        >  2:97264/1‑140 (MQ=255)                                                                        caactcacGGAGTGCGTTTTGTCGGTGAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATc                                                        >  2:275498/8‑140 (MQ=255)                                                                          aCGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTg                                                      <  1:913145/140‑1 (MQ=255)                                                                           cGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGTCCAGATCTGt                                                     <  2:1200272/140‑1 (MQ=255)                                                                             cTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGcc                                                              >  2:555522/1‑129 (MQ=255)                                                                             cTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGcc                                                              <  1:555522/129‑1 (MQ=255)                                                                               cGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCGAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCg                                                 <  1:1152502/140‑1 (MQ=255)                                                                               cGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCg                                                 >  2:4351/1‑140 (MQ=255)                                                                                ggAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGt                                                <  1:1237414/140‑1 (MQ=255)                                                                                ggAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGt                                                <  1:292578/140‑1 (MQ=255)                                                                                  aGTGCGTTTTGTCGGTAAATACGCCGGTCGTGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCa                                              >  1:1210871/1‑140 (MQ=255)                                                                                  aGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCa                                              <  1:638183/140‑1 (MQ=255)                                                                                      cGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGt                                          >  2:323489/1‑140 (MQ=255)                                                                                      cGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGt                                          >  2:900307/1‑140 (MQ=255)                                                                                       gTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTa                                         <  2:1228410/140‑1 (MQ=255)                                                                                       gTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTa                                         <  2:1135980/140‑1 (MQ=255)                                                                                       gTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTa                                         <  1:900363/140‑1 (MQ=255)                                                                                        ttttGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTaa                                        >  1:1035706/1‑140 (MQ=255)                                                                                        ttttGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTaa                                        >  2:826291/1‑140 (MQ=255)                                                                                         tttGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAAt                                       <  2:1399731/140‑1 (MQ=255)                                                                                           tGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCt                                     <  1:1012842/140‑1 (MQ=255)                                                                                              cGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTg                                  <  2:37804/140‑1 (MQ=255)                                                                                              cGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTg                                  >  2:667211/1‑140 (MQ=255)                                                                                              cGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTg                                  <  1:1374491/140‑1 (MQ=255)                                                                                              cGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTg                                  <  1:900307/140‑1 (MQ=255)                                                                                                gTAAATACGCCGGTCGTGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTgta                                <  2:1210871/140‑1 (MQ=255)                                                                                                gTAAATACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTgta                                <  2:500221/140‑1 (MQ=255)                                                                                                  aaaTACGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTgtagt                              <  1:323489/140‑1 (MQ=255)                                                                                                       cGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACg                          >  2:1168853/1‑139 (MQ=255)                                                                                                       cGCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACg                          <  1:1168853/139‑1 (MQ=255)                                                                                                        gCCGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGca                        <  1:876766/140‑1 (MQ=255)                                                                                                          cGGTCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGcaca                      <  1:1243214/140‑1 (MQ=255)                                                                                                             tCGGGCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAg                   >  1:579028/1‑140 (MQ=255)                                                                                                               gggCAAATTTCGACCAGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCg                 >  1:123847/1‑140 (MQ=255)                                                                                                                              aGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCa  <  2:925764/140‑1 (MQ=255)                                                                                                                              aGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCa  <  1:4351/140‑1 (MQ=255)                                                                                                                              aGGTCACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCa  <  1:101341/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        GGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCA  >  NC_000913/2399451‑2399715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑