Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,414,193	A→G	T242A (ACC→GCC)	ackA →	acetate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,414,193	0	A	G	100.0%	220.4 / NA	63	T242A (ACC→GCC)	ackA	acetate kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/34);  total (29/34)

GAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTACCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCTGACCGAAGTGACCAGCGAC  >  NC_000913/2414060‑2414324                                                                                                                                      |                                                                                                                                    gAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGtt                                                                                                                               <  1:826087/140‑1 (MQ=255)  aaCAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTc                                                                                                                              <  1:1323254/140‑1 (MQ=255)     aaaCCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTgg                                                                                                                           <  2:738125/140‑1 (MQ=255)       aCCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTg                                                                                                                         >  1:1075934/1‑140 (MQ=255)          ggTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGata                                                                                                                      <  2:824395/140‑1 (MQ=255)            tAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGtt                                                                                                                               <  1:1410088/129‑1 (MQ=255)            tAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGtt                                                                                                                               >  2:1410088/1‑129 (MQ=255)               aagaaCTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGAt                                                                                                                 <  2:861803/140‑1 (MQ=255)                 gaaCTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATcc                                                                                                               >  1:635591/1‑140 (MQ=255)                     tGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCg                                                                                                           >  1:492752/1‑140 (MQ=255)                      gAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGa                                                                                                          >  1:904924/1‑140 (MQ=255)                       aaCATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGat                                                                                                         <  2:904924/140‑1 (MQ=255)                       aaCATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGat                                                                                                         <  1:570129/140‑1 (MQ=255)                       aaCATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGat                                                                                                         <  1:384836/140‑1 (MQ=255)                         catcatCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGatca                                                                                                       >  1:591309/1‑140 (MQ=255)                            catcaCCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGat                                                                                                         <  2:509694/135‑1 (MQ=255)                            catcaCCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGat                                                                                                         >  1:509694/1‑135 (MQ=255)                            catcaCCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCt                                                                                                    >  2:61011/1‑140 (MQ=255)                               caCCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTcc                                                                                                 <  2:1075934/140‑1 (MQ=255)                               caCCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTcc                                                                                                 <  1:479400/140‑1 (MQ=255)                               caCCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTcc                                                                                                 <  1:746082/140‑1 (MQ=255)                                    gCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTg                                                                                            >  2:149857/1‑140 (MQ=255)                                      cACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCt                                                                                             <  2:1051959/137‑1 (MQ=255)                                      cACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCt                                                                                             >  1:1051959/1‑137 (MQ=255)                                        ccTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACg                                                                                        <  2:612588/140‑1 (MQ=255)                                        ccTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACg                                                                                        <  2:200520/140‑1 (MQ=255)                                         cTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGa                                                                                       <  2:959766/140‑1 (MQ=255)                                         cTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGa                                                                                       <  2:635591/140‑1 (MQ=255)                                         cTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGa                                                                                       <  2:591309/140‑1 (MQ=255)                                         cTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGa                                                                                       >  2:274168/1‑140 (MQ=255)                                            ggCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGacac                                                                                    >  1:1292138/1‑140 (MQ=255)                                             gCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGTGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACAcc                                                                                   <  2:6190/140‑1 (MQ=255)                                             gCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACAcc                                                                                   <  1:384674/140‑1 (MQ=255)                                             gCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACAcc                                                                                   <  1:1365257/140‑1 (MQ=255)                                               aaCGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCt                                                                                 >  1:584986/1‑140 (MQ=255)                                               aaCGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCt                                                                                 >  1:373020/1‑140 (MQ=255)                                                 cGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGa                                                                                       >  2:984349/1‑132 (MQ=255)                                                 cGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGa                                                                                       <  1:984349/132‑1 (MQ=255)                                                 cGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTgg                                                                               >  1:288416/1‑140 (MQ=255)                                                       ttCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGa                                                                         >  1:1146388/1‑140 (MQ=255)                                                                tGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACg                                                                >  2:642064/1‑140 (MQ=255)                                                                tGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACg                                                                >  2:362995/1‑140 (MQ=255)                                                                     tCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATc                                                           >  1:1007173/1‑140 (MQ=255)                                                                          aaaggTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATcaacaa                                                      >  2:830219/4‑140 (MQ=255)                                                                           aCGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACaaa                                                     <  2:584986/140‑1 (MQ=255)                                                                           aCGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACaaa                                                     <  2:373020/140‑1 (MQ=255)                                                                                aaaTGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAActgct                                                <  2:219392/140‑1 (MQ=255)                                                                                      gTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCaa                                          <  1:470162/140‑1 (MQ=255)                                                                                        tGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAag                                        >  2:1310754/1‑140 (MQ=255)                                                                                             ccTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCt                                   <  2:492752/140‑1 (MQ=255)                                                                                               tctATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTgg                                 <  2:1292138/140‑1 (MQ=255)                                                                                                    gggCCTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCctgc                            <  1:149857/140‑1 (MQ=255)                                                                                                        cTGACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTggg                        >  1:1191877/1‑140 (MQ=255)                                                                                                          gACCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTc                      >  2:1326664/1‑140 (MQ=255)                                                                                                           aCCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCt                     >  2:571224/1‑140 (MQ=255)                                                                                                           aCCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCt                     >  2:323687/1‑140 (MQ=255)                                                                                                           aCCCCGCTGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCt                     >  1:421801/1‑140 (MQ=255)                                                                                                                  tGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCTGACCGaa              <  1:642064/140‑1 (MQ=255)                                                                                                                  tGGAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCTGACCGaa              <  1:362995/140‑1 (MQ=255)                                                                                                                    gAAGGTCTGGTCATGGGTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCTGACCGAAGt            <  2:1146388/140‑1 (MQ=255)                                                                                                                       ggTCTGGTCATGGGTGCCCGTCCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCTGACCGAAGTGAc         >  2:445093/1‑140 (MQ=255)                                                                                                                                   ggTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCTGACCGAAGTGACCAGCGAc  <  1:960559/135‑1 (MQ=255)                                                                                                                                   ggTGCCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCTGACCGAAGTGACCAGCGAc  >  2:960559/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                    GAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACGGTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATGGGTACCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAACAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCTGACCGAAGTGACCAGCGAC  >  NC_000913/2414060‑2414324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑