Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,595,690	T→C	E57G (GAA→GGA)	tmcA ←	tRNA(Met) cytidine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,595,690	0	T	C	100.0%	235.3 / NA	66	E57G (GAA→GGA)	tmcA	tRNA(Met) cytidine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/45);  total (21/45)

CCAGCCAGCTTCCCGCTTTCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTTCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCG  >  NC_000913/2595553‑2595825                                                                                                                                          |                                                                                                                                        ccagccagCTTCCCGCTTTCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCa                                                                                                                                       >  1:789072/1‑140 (MQ=255)    gccagcTTCCCGCTTTCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTTTAGTGCCGAGGGGGAACAGTGGGTTCCAGCa                                                                                                                                    >  2:503303/1‑140 (MQ=255)     ccagcTTCCCGCTTTCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAAAACCGCATGCCGGAACTCGCGCCCAAATAAAGGTTGTAGTGCCGAGGGAGAAAAGTGGGTTCCAGCAt                                                                                                                                   >  2:1386549/1‑140 (MQ=255)      cagcTTCCCGCTTTCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTTGAATAACGCCTGCCGGTACTCCCGGCCAAGTAAAGATTTTTGGGGCGAGGGAGAACAGTGGGTTCCAGGAtt                                                                                                                                  >  2:601572/1‑139 (MQ=255)      cagcTTCCCGCTTTCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATc                                                                                                                                  >  2:274292/1‑140 (MQ=255)            cccGCTTTCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCcg                                                                                                                            <  2:137151/140‑1 (MQ=255)                 tttCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCgcggcg                                                                                                                       <  1:805007/140‑1 (MQ=255)                 tttCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCgcggcg                                                                                                                       <  1:601572/140‑1 (MQ=255)                  ttCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGa                                                                                                                      <  1:1328635/140‑1 (MQ=255)                         ttCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCcac                                                                                                               <  1:274292/140‑1 (MQ=255)                          tCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCcaca                                                                                                              >  1:344453/1‑140 (MQ=255)                           ccGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGCCCAAAACCGCACGCCCGGACTCCCGCCCAAAGAAAGTTTTTAGTGCCCCGGGAGGACAATGGGTTCCAGCATCTGGCCGCGGCGAAATCcacac                                                                                                             >  2:1385252/1‑139 (MQ=255)                              cTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCa                                                                                                          <  2:425021/140‑1 (MQ=255)                                 aGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGCAAAGTTTGTAGGGGCGAGGGGGGACAGAGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTc                                                                                                       >  2:12026/1‑140 (MQ=255)                                  gTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCg                                                                                                      <  2:455708/140‑1 (MQ=255)                                   tGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGc                                                                                                     <  2:789072/140‑1 (MQ=255)                                    gCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGcc                                                                                                    <  2:683996/140‑1 (MQ=255)                                    gCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGcc                                                                                                    <  1:12026/140‑1 (MQ=255)                                        cAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGt                                                                                                <  1:1254405/140‑1 (MQ=255)                                         aaaGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTa                                                                                               <  1:1386549/140‑1 (MQ=255)                                            ggCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAgg                                                                                            <  2:234008/140‑1 (MQ=255)                                            ggCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAgg                                                                                            <  1:503303/140‑1 (MQ=255)                                             gCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCg                                                                                                      >  1:947461/1‑129 (MQ=255)                                             gCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCg                                                                                                      <  2:947461/129‑1 (MQ=255)                                              cAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCa                                                                                          <  1:603303/140‑1 (MQ=255)                                                 cGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCa                                                                                       <  1:792071/140‑1 (MQ=255)                                                  ggCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCAc                                                                                      >  2:510365/1‑140 (MQ=255)                                                   gCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACg                                                                                     <  2:870081/140‑1 (MQ=255)                                                   gCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACg                                                                                     <  2:158153/140‑1 (MQ=255)                                                   gCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACg                                                                                     <  1:784626/140‑1 (MQ=255)                                                   gCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACg                                                                                     <  1:510365/140‑1 (MQ=255)                                                   gCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACg                                                                                     <  2:344453/140‑1 (MQ=255)                                                   gCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACg                                                                                     <  1:1370909/140‑1 (MQ=255)                                                    cATCAAAGCCGTGGCGGGCGTCTAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGc                                                                                    <  1:1010577/140‑1 (MQ=255)                                                             cGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAaga                                                                           <  2:1000824/140‑1 (MQ=255)                                                               tGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAagagg                                                                         >  1:934326/1‑139 (MQ=255)                                                               tGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGt                                                                         <  1:520088/140‑1 (MQ=255)                                                               tGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTCCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGt                                                                         <  2:1255206/140‑1 (MQ=255)                                                                    ggcgTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCt                                                                    <  1:1385252/140‑1 (MQ=255)                                                                            aTACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAacac                                                            <  2:201624/140‑1 (MQ=255)                                                                            aTACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAacac                                                            <  2:481034/140‑1 (MQ=255)                                                                               ccGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCaa                                                         >  2:1019958/1‑140 (MQ=255)                                                                                cGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAAc                                                        >  2:1366170/1‑140 (MQ=255)                                                                                   aTGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCct                                                     <  1:1108732/140‑1 (MQ=255)                                                                                    tGCCGGGACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAacac                                                            >  1:1038304/1‑132 (MQ=255)                                                                                    tGCCGGGACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAacac                                                            <  2:1038304/132‑1 (MQ=255)                                                                                    tGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCctc                                                    >  1:1040398/1‑140 (MQ=255)                                                                                     gCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCctct                                                   >  2:702745/1‑140 (MQ=255)                                                                                     gCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCC‑‑GGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTc                                                 >  2:1376719/1‑140 (MQ=255)                                                                                       cGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCa                                                                  >  2:806022/1‑123 (MQ=255)                                                                                       cGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCa                                                                  <  1:806022/123‑1 (MQ=255)                                                                                          aaCTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTcc                                                <  2:677893/138‑1 (MQ=255)                                                                                          aaCTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTcc                                                >  1:677893/1‑138 (MQ=255)                                                                                           aCTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCg                                             <  2:1040398/140‑1 (MQ=255)                                                                                               gcgcCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCa                                         >  1:1142841/1‑140 (MQ=255)                                                                                                             tttGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAgcgg                           >  1:1409364/1‑140 (MQ=255)                                                                                                             tttGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAgcgg                           >  2:1222673/1‑140 (MQ=255)                                                                                                               tGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAgcggcg                         <  2:1327918/140‑1 (MQ=255)                                                                                                               tGTAGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAgcggcg                         <  1:1063022/140‑1 (MQ=255)                                                                                                                gTAGTGCC‑‑GGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGAt                      <  1:1376719/140‑1 (MQ=255)                                                                                                                  aGTGCCGAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGAt                      >  2:745010/1‑140 (MQ=255)                                                                                                                      cagAGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCt                  >  1:33702/3‑140 (MQ=255)                                                                                                                         aGGGAGAACAGTGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCa               >  1:955490/1‑140 (MQ=255)                                                                                                                                  aGTGGTTTCCAGCATCTGGCCGCGGCGAAATC‑‑CAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTg    <  1:477808/140‑1 (MQ=255)                                                                                                                                    tGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGAt                      >  2:353093/1‑122 (MQ=255)                                                                                                                                    tGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGAt                      <  1:353093/122‑1 (MQ=255)                                                                                                                                    tGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCtt                 >  1:886299/1‑127 (MQ=255)                                                                                                                                    tGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCtt                 <  2:886299/127‑1 (MQ=255)                                                                                                                                    tGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTg    <  1:1019958/140‑1 (MQ=255)                                                                                                                                    tGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTg    <  1:267463/140‑1 (MQ=255)                                                                                                                                    tGGTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTg    >  1:348880/1‑140 (MQ=255)                                                                                                                                      gTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTgcg  <  1:1234061/140‑1 (MQ=255)                                                                                                                                      gTTTCCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTgcg  <  2:1142841/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CCAGCCAGCTTCCCGCTTTCAACGTTCCGCTAAGTGCGGCAAAGGCAGCGGCATCAAAGCCGTGGCGGGCGTCGAATACCGCATGCCGGAACTCGCGCCCAAGTAAAGTTTGTAGTGCCGAGGGAGAACAGTGGTTTTCAGCATCTGGCCGCGGCGAAATCCACAGCCAGTCGCCAGGTAAGGCATCACGCAACTTAAGAGTATGCTCAAAACACCAACCCTCTTCCCCGCTCAACACCAGCAAGCGGCGGATCCCTTCACGTTTCATTTGCG  >  NC_000913/2595553‑2595825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑