Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,706,111	G→A	F338F (TTC→TTT)	lepA ←	elongation factor 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,706,111	0	G	A	100.0%	342.0 / NA	95	F338F (TTC→TTT)	lepA	elongation factor 4
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/46);  total (49/46)

TAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTG  >  NC_000913/2705981‑2706245                                                                                                                                   |                                                                                                                                       tAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAg                                                                                                                               >  2:709825/1‑140 (MQ=255)     ttCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCa                                                                                                                           >  1:461136/1‑140 (MQ=255)      tctcTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAg                                                                                                                          <  2:694631/140‑1 (MQ=255)       ctctTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAgc                                                                                                                         >  1:171922/1‑140 (MQ=255)             aCGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTg                                                                                                                   >  1:322495/1‑140 (MQ=255)                    ttCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTTACGTTCCAGACGTTCCTGGGTGATCTCCATGTGCAGCAGGCCGAGGGAGCCGCAGCGAAAAACCAAACCCCGCGCGCTGGGGCttt                                                                                                            >  2:961328/1‑140 (MQ=255)                     tCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTc                                                                                                           <  1:709825/140‑1 (MQ=255)                       aaCTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                                                                         <  1:1067406/140‑1 (MQ=255)                       aaCTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                                                                         <  2:1420177/140‑1 (MQ=255)                       aaCTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                                                                         <  1:377260/140‑1 (MQ=255)                         cTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGc                                                                                                       <  1:1260085/140‑1 (MQ=255)                         cTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGc                                                                                                       <  1:1274464/140‑1 (MQ=255)                         cTTCATACACTACGGTCGGCGCAGTGGTGATAAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGc                                                                                                       <  1:964132/140‑1 (MQ=255)                           tCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTc                                                                                                     <  1:1324575/140‑1 (MQ=255)                            cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCa                                                                                                    >  2:199818/1‑140 (MQ=255)                            cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCa                                                                                                    <  2:461136/140‑1 (MQ=255)                             aTACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                                                                         >  2:577102/1‑134 (MQ=255)                             aTACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                                                                         <  1:577102/134‑1 (MQ=255)                                 acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGaa                                                                                               <  2:322495/140‑1 (MQ=255)                                   tACGGTCGGCGCAGTGGTGATCGGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa                                                                                             >  1:793941/1‑140 (MQ=255)                                    aCGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCa                                                                                                    >  2:815/1‑132 (MQ=255)                                    aCGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCa                                                                                                    <  1:815/132‑1 (MQ=255)                                     cGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt                                                                                           >  2:869442/1‑140 (MQ=255)                                     cGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt                                                                                           >  1:53945/1‑140 (MQ=255)                                       gTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                                                                         <  1:177203/124‑1 (MQ=255)                                       gTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                                                                         >  2:177203/1‑124 (MQ=255)                                       gTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGa                                                                                         >  2:807862/1‑140 (MQ=255)                                        tCGGCGCAGTGGGGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAg                                                                                        >  2:223480/1‑140 (MQ=255)                                           gcgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCa                                                                                     >  2:907007/1‑140 (MQ=255)                                           gcgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCa                                                                                     >  2:563332/1‑140 (MQ=255)                                            cgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCAt                                                                                    <  1:1177175/140‑1 (MQ=255)                                             gcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATc                                                                                   >  2:921322/1‑140 (MQ=255)                                                gtggtgATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGtt                                                                                <  1:14609/140‑1 (MQ=255)                                                 tggtgATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTc                                                                               >  1:283051/1‑140 (MQ=255)                                                    tgATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAgg                                                                            <  2:969394/140‑1 (MQ=255)                                                     gATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                                                                           <  2:886565/140‑1 (MQ=255)                                                      aTCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCt                                                                          >  1:1247813/1‑140 (MQ=255)                                                      aTCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCt                                                                          >  2:583810/1‑140 (MQ=255)                                                        cagatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGa                                                                        <  1:611695/140‑1 (MQ=255)                                                         agatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAg                                                                       <  2:816581/140‑1 (MQ=255)                                                           atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGGGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGtt                                                                     >  1:1319051/1‑140 (MQ=255)                                                           atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                                                                           >  1:181246/1‑134 (MQ=255)                                                           atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                                                                           <  2:181246/134‑1 (MQ=255)                                                           atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGtt                                                                     >  2:442504/1‑140 (MQ=255)                                                           atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGtt                                                                     >  2:347053/1‑140 (MQ=255)                                                           atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGtt                                                                     >  2:1347943/1‑140 (MQ=255)                                                             ccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTa                                                                   <  1:442504/140‑1 (MQ=255)                                                               agatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGGGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTAcc                                                                 <  2:1319051/140‑1 (MQ=255)                                                                gatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTAccc                                                                >  1:1108859/1‑140 (MQ=255)                                                                     tATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgcgc                                                           >  2:287893/1‑140 (MQ=255)                                                                     tATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgcgc                                                           >  2:1394974/1‑140 (MQ=255)                                                                      aTTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGcgcg                                                          >  1:746538/1‑140 (MQ=255)                                                                           cGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACg                                                     <  1:287893/140‑1 (MQ=255)                                                                            gTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACgg                                                    >  1:677451/1‑140 (MQ=255)                                                                             ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGa                                                   <  2:1247813/140‑1 (MQ=255)                                                                             ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGa                                                   <  2:793941/140‑1 (MQ=255)                                                                             ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGa                                                   <  1:563332/140‑1 (MQ=255)                                                                              tCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGaa                                                  <  2:171922/140‑1 (MQ=255)                                                                                cAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTCAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGCAgg                                                >  2:1312100/1‑140 (MQ=255)                                                                                  gACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCt                                              >  2:1214268/1‑140 (MQ=255)                                                                                       tCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATa                                         <  2:53945/140‑1 (MQ=255)                                                                                         cTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAgt                                       <  1:869442/140‑1 (MQ=255)                                                                                                atCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGa                                >  1:1274522/1‑140 (MQ=255)                                                                                                atCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGa                                >  1:918061/1‑140 (MQ=255)                                                                                                 tctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGaa                               >  2:49116/1‑140 (MQ=255)                                                                                                  ctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAAc                              <  2:807107/140‑1 (MQ=255)                                                                                                  ctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAAc                              <  2:1108859/140‑1 (MQ=255)                                                                                                  ctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAAc                              <  1:1347943/140‑1 (MQ=255)                                                                                                  ctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAAc                              >  1:880462/1‑140 (MQ=255)                                                                                                       tgtgCAGCAGGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGTATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTAcc                         <  1:538323/140‑1 (MQ=255)                                                                                                           cagcagGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGa                     >  1:496153/1‑140 (MQ=255)                                                                                                           cagcagGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGa                     >  2:1128970/1‑140 (MQ=255)                                                                                                           cagcagGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGa                     >  2:1329459/1‑140 (MQ=255)                                                                                                           cagcagGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGACTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGa                     >  2:1368004/1‑140 (MQ=255)                                                                                                            agcagGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGaa                    >  2:1385714/1‑140 (MQ=255)                                                                                                             gcagGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACtt                            <  1:1370607/131‑1 (MQ=255)                                                                                                             gcagGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACtt                            >  2:1370607/1‑131 (MQ=255)                                                                                                             gcagGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAAc                   >  2:530517/1‑140 (MQ=255)                                                                                                               agGCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAg                 >  2:649342/1‑140 (MQ=255)                                                                                                                ggCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGa                <  1:199818/140‑1 (MQ=255)                                                                                                                ggCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGa                <  2:746538/140‑1 (MQ=255)                                                                                                                ggCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGa                <  1:649342/140‑1 (MQ=255)                                                                                                                 gCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAc               <  2:1274522/140‑1 (MQ=255)                                                                                                                 gCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAc               <  1:1394974/140‑1 (MQ=255)                                                                                                                 gCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAc               <  1:1312100/140‑1 (MQ=255)                                                                                                                 gCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAc               >  2:1060654/1‑140 (MQ=255)                                                                                                                 gCCGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAc               <  1:1128970/140‑1 (MQ=255)                                                                                                                  ccGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGACTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAcc              <  1:1368004/140‑1 (MQ=255)                                                                                                                   cGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCg             >  2:647047/1‑140 (MQ=255)                                                                                                                   cGAGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCg             >  1:727666/1‑140 (MQ=255)                                                                                                                     aGGAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGc           >  2:127797/1‑140 (MQ=255)                                                                                                                      ggAAGCCGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCg          <  1:961328/140‑1 (MQ=255)                                                                                                                            cGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGa                >  2:736321/1‑128 (MQ=255)                                                                                                                            cGCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGa                <  1:736321/128‑1 (MQ=255)                                                                                                                             gCAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCt   >  2:1308572/1‑140 (MQ=255)                                                                                                                              cAGCGAAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTg  <  1:347053/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       TAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTG  >  NC_000913/2705981‑2706245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑