Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,766,322 T→C H135H (CAT→CAC)  rnlA → CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,766,3220TC100.0% 359.8 / NA 100H135H (CAT→CAC) rnlACP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (65/35);  total (65/35)

GCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCC  >  NC_000913/2766200‑2766457
                                                                                                                          |                                                                                                                                       
gctgAATTTGAGCAGGTAAATATGGTACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAat                                                                                                                        >  1:185188/1‑140 (MQ=255)
   gAATTTGAGCAGGTAAATATGGTACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAattat                                                                                                                     >  1:122999/1‑140 (MQ=255)
      tttGAGCAGGTAAATATGGTACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTc                                                                                                                  >  1:1079580/1‑140 (MQ=255)
      tttGAGCAGGTAAATATGGTACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTc                                                                                                                  >  2:173465/1‑140 (MQ=255)
        tGAGCAGGTAAATATGGTACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTcca                                                                                                                <  1:315494/140‑1 (MQ=255)
              ggTAAATATGGTACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCt                                                                                                          >  1:297126/1‑140 (MQ=255)
                     atGGTACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGa                                                                                                   <  2:122999/140‑1 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGTATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGaa                                                                                               >  1:456047/1‑140 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGa                                                                                                   >  1:1152766/1‑136 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGa                                                                                                   <  2:1152766/136‑1 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGaa                                                                                               >  2:957563/1‑140 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGaa                                                                                               >  2:451361/1‑140 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGaa                                                                                               >  2:381771/1‑140 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGaa                                                                                               >  2:271604/1‑140 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGaa                                                                                               >  2:1215976/1‑140 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGaa                                                                                               >  1:791742/1‑140 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGaa                                                                                               <  1:1215976/140‑1 (MQ=255)
                         tACTGCAAGGATTTGTAGAGACAAGCGTCCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGaa                                                                                               >  1:225547/1‑140 (MQ=255)
                           cTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAAtt                                                                                             <  1:909665/140‑1 (MQ=255)
                            tGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTg                                                                                            >  1:1383829/1‑140 (MQ=255)
                            tGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTg                                                                                            >  1:963326/1‑140 (MQ=255)
                            tGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTg                                                                                            >  1:713511/1‑140 (MQ=255)
                              cAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGAc                                                                                          >  1:876796/1‑140 (MQ=255)
                              cAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGAc                                                                                          >  1:823001/1‑140 (MQ=255)
                                aGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACtg                                                                                        >  2:338482/1‑140 (MQ=255)
                                aGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACtg                                                                                        <  2:712674/140‑1 (MQ=255)
                                 ggATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACtgt                                                                                       >  2:1087081/1‑140 (MQ=255)
                                   aTTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGa                                                                                     >  1:221947/1‑140 (MQ=255)
                                      tGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGcc                                                                                  >  1:997145/1‑140 (MQ=255)
                                        tAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTg                                                                                <  1:196649/140‑1 (MQ=255)
                                        tAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTg                                                                                <  2:1079580/140‑1 (MQ=255)
                                        tAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTg                                                                                >  1:309198/1‑140 (MQ=255)
                                        tAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTg                                                                                <  1:451361/140‑1 (MQ=255)
                                        tAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTg                                                                                >  2:1419239/1‑140 (MQ=255)
                                        tAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTg                                                                                <  2:185188/140‑1 (MQ=255)
                                        tAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTg                                                                                <  1:381771/140‑1 (MQ=255)
                                          gagaCAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCa                                                                              <  1:214675/140‑1 (MQ=255)
                                          gagaCAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCa                                                                              >  1:333838/1‑140 (MQ=255)
                                           agaCAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCat                                                                             <  2:1383829/140‑1 (MQ=255)
                                             aCAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCatat                                                                           <  1:338482/140‑1 (MQ=255)
                                             aCAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCatat                                                                           <  1:659042/140‑1 (MQ=255)
                                             aCAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCatat                                                                           <  2:1243529/140‑1 (MQ=255)
                                             aCAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCatat                                                                           >  1:368887/1‑140 (MQ=255)
                                               aaGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATca                                                                         >  1:1339926/1‑140 (MQ=255)
                                                aGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATcac                                                                        <  2:823001/140‑1 (MQ=255)
                                                  cGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCat                                                                             >  1:438651/1‑133 (MQ=255)
                                                  cGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCat                                                                             <  2:438651/133‑1 (MQ=255)
                                                       tACCTGTATTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAGCAGGt                                                                 >  2:1264006/1‑140 (MQ=255)
                                                       tACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCATAACAGGt                                                                 <  1:1087081/140‑1 (MQ=255)
                                                       tACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGt                                                                 >  1:254048/1‑140 (MQ=255)
                                                          cTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAg                                                              <  2:225547/140‑1 (MQ=255)
                                                           tGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGc                                                             <  1:303908/140‑1 (MQ=255)
                                                            gTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCt                                                            >  2:236213/1‑140 (MQ=255)
                                                              aCTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTcc                                                          <  2:221947/140‑1 (MQ=255)
                                                              aCTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTcc                                                          <  1:1419239/140‑1 (MQ=255)
                                                               cTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCa                                                         >  1:1145479/1‑140 (MQ=255)
                                                                 tGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGa                                                       >  1:296829/1‑140 (MQ=255)
                                                                   aGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGt                                                                 >  2:1164546/1‑128 (MQ=255)
                                                                   aGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGt                                                                 <  1:1164546/128‑1 (MQ=255)
                                                                   aGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGAtt                                                     >  2:345751/1‑140 (MQ=255)
                                                                   aGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGAtt                                                     >  1:469914/1‑140 (MQ=255)
                                                                   aGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTACATATCACAACAGGTAAGCTCCAGAtt                                                     >  1:278491/1‑140 (MQ=255)
                                                                   aGCTATCAGCAGATGAATCGCATATAG‑GTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTc                                                    >  1:845088/1‑140 (MQ=255)
                                                                     cTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCa                                                   >  1:609948/1‑140 (MQ=255)
                                                                       aTCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAgg                                                 >  1:162892/1‑140 (MQ=255)
                                                                       aTCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAgg                                                 >  2:387320/1‑140 (MQ=255)
                                                                       aTCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAgg                                                 >  2:390736/1‑140 (MQ=255)
                                                                        tCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAggg                                                >  1:1189200/1‑140 (MQ=255)
                                                                             agATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGAc                                           <  1:957563/140‑1 (MQ=255)
                                                                             agATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGAc                                           <  2:469914/140‑1 (MQ=255)
                                                                              gATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGAcc                                          >  2:661034/1‑140 (MQ=255)
                                                                               aTGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCg                                         >  2:1176930/1‑140 (MQ=255)
                                                                                tGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGc                                        <  1:390736/140‑1 (MQ=255)
                                                                                    tCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTTTGAGCCTACATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTc                                    <  2:278491/140‑1 (MQ=255)
                                                                                    tCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTc                                    <  1:1176930/140‑1 (MQ=255)
                                                                                    tCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTc                                    <  2:368887/140‑1 (MQ=255)
                                                                                            agagTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACa                            >  1:1359986/1‑140 (MQ=255)
                                                                                              agTTCAGAGAACACTCTCGTAGCGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTAACAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACaga                          >  2:1274869/1‑140 (MQ=255)
                                                                                               gTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCg                                         <  1:1348754/124‑1 (MQ=255)
                                                                                               gTTCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCg                                         >  2:1348754/1‑124 (MQ=255)
                                                                                                ttCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGt                        >  1:421172/1‑140 (MQ=255)
                                                                                                ttCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGt                        >  2:1333824/1‑140 (MQ=255)
                                                                                                ttCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGt                        >  1:1188739/1‑140 (MQ=255)
                                                                                                 tCAGAGAACACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGtt                       >  1:1333192/1‑140 (MQ=255)
                                                                                                       aaCACTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACg                 <  2:309198/140‑1 (MQ=255)
                                                                                                        acacTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGt                >  2:254671/1‑140 (MQ=255)
                                                                                                          acTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGttt              >  1:1002516/1‑140 (MQ=255)
                                                                                                          acTCTCGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGttt              >  1:940811/1‑140 (MQ=255)
                                                                                                            tctcGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTaa            <  2:333838/140‑1 (MQ=255)
                                                                                                            tctcGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTaa            >  2:560414/1‑140 (MQ=255)
                                                                                                            tctcGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTaa            >  1:1276610/1‑140 (MQ=255)
                                                                                                            tctcGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTaa            >  2:1030451/1‑140 (MQ=255)
                                                                                                            tctcGTAACGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTaa            <  2:880380/140‑1 (MQ=255)
                                                                                                                    cGCTCACACCGTAGTGTGGAAAATTATTTCCATCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAg    >  1:1424903/1‑140 (MQ=255)
                                                                                                                    cGCTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAg    <  1:254671/140‑1 (MQ=255)
                                                                                                                      cTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGcc  >  1:193904/1‑140 (MQ=255)
                                                                                                                      cTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGcc  >  2:295664/1‑140 (MQ=255)
                                                                                                                      cTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGcc  >  1:431780/1‑140 (MQ=255)
                                                                                                                      cTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGcc  >  2:595491/1‑140 (MQ=255)
                                                                                                                      cTCACACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGcc  >  2:785084/1‑140 (MQ=255)
                                                                                                                          |                                                                                                                                       
GCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAGCTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCC  >  NC_000913/2766200‑2766457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑