Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,719	C→T	A765T (GCA→ACA)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,719	0	C	T	100.0%	231.8 / NA	65	A765T (GCA→ACA)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/32);  total (33/32)

ACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCA  >  NC_000913/2819589‑2819853                                                                                                                                   |                                                                                                                                       aCTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGa                                                                                                                               >  2:659466/1‑140 (MQ=255) aCTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGa                                                                                                                               >  1:311520/1‑140 (MQ=255)     ccGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGa                                                                                                                           >  2:1103252/1‑140 (MQ=255)     ccGTTGCCAGCACGATAATTGTCGACCCCAGCTGA‑TTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGAt                                                                                                                          <  2:911231/140‑1 (MQ=255)      cGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGAt                                                                                                                          <  1:664637/140‑1 (MQ=255)        ttGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGAttt                                                                                                                        >  1:378910/1‑140 (MQ=255)           ccAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTgcg                                                                                                                     <  2:598429/140‑1 (MQ=255)           ccAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTgcg                                                                                                                     <  2:1179841/140‑1 (MQ=255)           ccAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTgcg                                                                                                                     <  2:1174086/140‑1 (MQ=255)            cAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTgcgc                                                                                                                    <  2:311520/140‑1 (MQ=255)               cACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGctc                                                                                                                  >  2:816240/1‑139 (MQ=255)               cACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGctc                                                                                                                  <  1:816240/139‑1 (MQ=255)                aCGATAATTGTCGACCCCAGCTGATTTTTTAAATCTTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCtctc                                                                                                                <  2:986135/140‑1 (MQ=255)                          tCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTACGGCa                                                                                                      >  2:737345/1‑140 (MQ=255)                            gACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCtctc                                                                                                                >  1:1159264/1‑128 (MQ=255)                            gACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCtctc                                                                                                                <  2:1159264/128‑1 (MQ=255)                              ccccAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCtt                                                                                                  <  2:704822/140‑1 (MQ=255)                              ccccAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCACTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCtt                                                                                                  <  1:1382770/140‑1 (MQ=255)                                 cAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGtt                                                                                               >  1:65773/1‑140 (MQ=255)                                        ttttttAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAg                                                                                        <  2:1025115/140‑1 (MQ=255)                                         tttttAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGc                                                                                       <  1:758452/140‑1 (MQ=255)                                         tttttAAATCGTCAACCATGGCACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAATACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGc                                                                                       <  2:1298733/140‑1 (MQ=255)                                               aaTCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACTCCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCtgttgt                                                                                 <  1:231927/140‑1 (MQ=255)                                                           tGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTcc                                                                     >  2:764370/1‑140 (MQ=255)                                                           tGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTcc                                                                     >  2:400587/1‑140 (MQ=255)                                                            ggTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCa                                                                    >  1:925613/1‑140 (MQ=255)                                                             gTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAg                                                                   <  2:65773/140‑1 (MQ=255)                                                               aCGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGTAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCt                                                                 <  1:1377958/140‑1 (MQ=255)                                                                cGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTg                                                                <  2:378910/140‑1 (MQ=255)                                                                     cATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGAcgcg                                                           >  1:438736/1‑140 (MQ=255)                                                                       ttttCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTa                                                         <  1:310902/140‑1 (MQ=255)                                                                       ttttCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTa                                                         <  2:582638/140‑1 (MQ=255)                                                                       ttttCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTa                                                         <  2:683362/140‑1 (MQ=255)                                                                       ttttCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTa                                                         <  1:588582/140‑1 (MQ=255)                                                                         ttCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACg                                                       <  1:1179930/140‑1 (MQ=255)                                                                             gCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCGTTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTa                                                         >  1:765661/1‑134 (MQ=255)                                                                             gCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCGTTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTa                                                         <  2:765661/134‑1 (MQ=255)                                                                               tCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAg                                                 >  2:859674/1‑140 (MQ=255)                                                                                      cGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAg                                          >  2:679940/1‑140 (MQ=255)                                                                                        cTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACCGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAgcg                                        >  1:484561/1‑140 (MQ=255)                                                                                          aaGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCa                                      <  2:438736/140‑1 (MQ=255)                                                                                                gCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTAt                                >  2:1317295/1‑140 (MQ=255)                                                                                                gCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTAt                                >  1:1078980/1‑140 (MQ=255)                                                                                                gCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTAt                                >  2:1211303/1‑140 (MQ=255)                                                                                                gCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTAt                                <  1:1425705/140‑1 (MQ=255)                                                                                                gCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTAt                                >  1:692851/1‑140 (MQ=255)                                                                                                gCTAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTAt                                >  2:717472/1‑140 (MQ=255)                                                                                                  tAACCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCa                              >  1:410344/1‑140 (MQ=255)                                                                                                   aaCCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAg                             <  2:925613/140‑1 (MQ=255)                                                                                                   aaCCAACAGCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAg                             <  1:1200587/140‑1 (MQ=255)                                                                                                           gCTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGatta                     <  2:665005/140‑1 (MQ=255)                                                                                                            cTTAACACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGattat                    >  2:954263/1‑140 (MQ=255)                                                                                                               aaCACCATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGc                 <  1:764370/140‑1 (MQ=255)                                                                                                                    cATTAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCg            <  1:771711/140‑1 (MQ=255)                                                                                                                      ttAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGcc          >  2:1234951/1‑140 (MQ=255)                                                                                                                      ttAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGcc          >  1:709357/1‑140 (MQ=255)                                                                                                                      ttAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGcc          >  2:461293/1‑140 (MQ=255)                                                                                                                      ttAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGcc          >  2:348038/1‑140 (MQ=255)                                                                                                                      ttAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCAcc          >  2:519039/1‑140 (MQ=255)                                                                                                                       tAACATCAATTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCt         >  1:211595/1‑140 (MQ=255)                                                                                                                              aaTTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCg            <  1:102583/130‑1 (MQ=255)                                                                                                                              aaTTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCg            >  2:102583/1‑130 (MQ=255)                                                                                                                              aaTTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTcagca  >  1:1271791/1‑140 (MQ=255)                                                                                                                              aaTTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTcagca  >  2:1064447/1‑140 (MQ=255)                                                                                                                              aaTTGTCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTcagca  >  1:233414/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                       ACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCA  >  NC_000913/2819589‑2819853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑