| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,667,893 | (C)5→6 | coding (672/1221 nt) | mlc ← | DNA‑binding transcriptional repressor Mlc |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,667,888 | 1 | . | C | 96.0% | 80.5 / ‑3.0 | 25 | coding (677/1221 nt) | mlc | DNA‑binding transcriptional repressor Mlc |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (11/13); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
CGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCG‑CCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGC > NC_000913/1667769‑1668005 | cGAGGCAGCCTTGATTCCCGCAATCACATCGTGTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTtgt < 2:278363/133‑1 (MQ=255)cGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTtgt > 1:1968/1‑133 (MQ=255) gacAGCCGTTTTTCCAGCAATAACAGTGTTTCCCATACGGGGCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGAt < 2:279987/131‑1 (MQ=255) ccGTGATTCGCGCAAGAACAGCGTGTCCTATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATa < 2:278318/133‑1 (MQ=255) ccGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATa < 1:624967/133‑1 (MQ=255) ttCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGcc < 2:11082/133‑1 (MQ=255) tCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCTCGCCCCCCACGTTGTGATCGATAACAACCGGAGTCACATGGCGCGccc > 1:183864/1‑133 (MQ=255) aCGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTg > 1:501727/1‑133 (MQ=255) gTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGc > 1:32738/1‑133 (MQ=255) gtgtgtgGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACa > 1:418715/1‑133 (MQ=255) gtgtgtgGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACa > 1:617268/1‑133 (MQ=255) ttCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCAt < 2:32738/133‑1 (MQ=255) ccACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCg > 1:126693/1‑133 (MQ=255) cGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCG‑CCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCa < 2:32382/133‑1 (MQ=255) gaCTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATg < 2:126693/133‑1 (MQ=255) tactGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATgcgc < 1:147/133‑1 (MQ=255) gcctgcGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATc < 2:607112/133‑1 (MQ=255) cctgcGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCa > 1:60526/1‑133 (MQ=255) ctgcGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCAt < 2:617268/133‑1 (MQ=255) tgcGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAACGCCGCTGCCATCGTCCCTGCGGTGATATCATg > 2:246397/1‑133 (MQ=255) cGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCt > 1:37362/1‑133 (MQ=255) cGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCt < 2:418715/133‑1 (MQ=255) cGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGGGAGGCACCAATCAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCt > 2:411963/1‑133 (MQ=255) cGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGc < 2:1968/133‑1 (MQ=255) cGGTAATGACGCCCGCCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGc < 2:110631/133‑1 (MQ=255) | CGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCG‑CCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGC > NC_000913/1667769‑1668005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |