| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,227,340 | (C)6→7 | pseudogene (1419/1436 nt) | mdtQ ← | putative multidrug resistance outer membrane protein MdtQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,227,334 | 1 | . | C | 88.0% | 73.5 / 4.1 | 25 | pseudogene (1425/1436 nt) | mdtQ | putative multidrug resistance outer membrane protein MdtQ |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (13/9); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑A‑CCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGAT > NC_000913/2227217‑2227460 | gcACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCCTATAATCAGCGTTTGTCC‑CCCCCCGCCCAACg > 2:254925/1‑133 (MQ=255)gcACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACg > 2:129021/1‑133 (MQ=255)gcACTCTTAGCCGATTCCTGAAACGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACg < 1:259126/133‑1 (MQ=255) cACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGc > 1:476074/1‑133 (MQ=255) aCTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCa < 2:476074/133‑1 (MQ=255) ctTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCa > 1:36115/1‑133 (MQ=255) ttGCCGATTCCCGAACCGCAGGGGGTAATACTCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGt < 1:249910/133‑1 (MQ=255) ttGCCGATCCCCGAATCGCAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGt < 1:178970/133‑1 (MQ=255) ccGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAg < 2:67096/133‑1 (MQ=255) aGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑A‑CCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATc < 2:473165/131‑1 (MQ=255) aGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑A‑CCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATc > 1:473165/1‑131 (MQ=255) gTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCa < 2:36115/133‑1 (MQ=255) tgCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACa > 1:533443/1‑133 (MQ=255) tgCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACa < 1:129021/133‑1 (MQ=255) tAACGCTGCATAAACTTTAAGTTTAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACg < 1:254925/133‑1 (MQ=255) aaCGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGc > 2:60866/1‑133 (MQ=255) aaTAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCgg > 2:487782/1‑133 (MQ=255) gAAGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGGCATTGGCCGGCTAACGGAGCGCGGGAATTCAGGCTTCGCTg > 2:199586/1‑133 (MQ=255) aaGGCGCATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGa > 1:518206/1‑133 (MQ=255) gcgcATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGAcgc > 1:2014/1‑133 (MQ=255) gcgcATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGAcgc > 1:153401/1‑133 (MQ=255) gcATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCgg > 1:598765/1‑133 (MQ=255) gcATATCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCgg > 2:137845/1‑133 (MQ=255) atatCATCAGCGTTTGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGa < 2:518206/133‑1 (MQ=255) tttGT‑ACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGACCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAatgat > 1:15826/1‑133 (MQ=255) | GCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGT‑A‑CCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGAT > NC_000913/2227217‑2227460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |