| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,765,794 | (C)7→8 | intergenic (‑18/‑124) | yfjM ← / → rnlA | CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,765,787 | 1 | . | C | 96.8% | 101.0 / NA | 32 | intergenic (‑11/‑131) | yfjM/rnlA | CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (7/23); minor base A (0/1); total (8/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATG > NC_000913/2765655‑2765906 | tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTTTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTg > 2:394067/1‑133 (MQ=255)tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTg > 2:88441/1‑133 (MQ=255) cTCGTTCTTTTTCGATGTTGAAATTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑ccc‑c < 1:230891/133‑1 (MQ=255) cTCGTTCTGTTTCGACGTAGAATTTTTTTTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑ccc‑c < 2:265288/133‑1 (MQ=255) cTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑ccc‑c < 1:318283/133‑1 (MQ=255) gTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑ccc‑cccc < 2:343534/133‑1 (MQ=255) gTTCTGTTTCGACGTAGAATTTGTTCTTGTTTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑ccc‑cccc < 1:266160/133‑1 (MQ=255) ttCGACGTAGCATTTCTTCTTGTCTGCTCGCGAATAATTACGAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGa < 2:167340/133‑1 (MQ=255) cGTAGACTTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATc < 2:576618/133‑1 (MQ=255) cGTAGAATTTGTTCTGGTCTGCTGACGAATAATTACTAAGTCTCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATc < 2:261904/133‑1 (MQ=255) aGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTg < 1:152555/133‑1 (MQ=255) gAATTTTTTCTTGGCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGc < 2:544977/133‑1 (MQ=255) aTTTTTTCTTTTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGccc < 1:394067/133‑1 (MQ=255) tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCACCCCGGTCGACCATCCTGCCCTATAGTTgg > 1:245309/1‑133 (MQ=255) ctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc < 2:562877/133‑1 (MQ=255) ctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc < 2:324577/133‑1 (MQ=255) ctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc < 2:141296/133‑1 (MQ=255) tgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccc < 1:559547/133‑1 (MQ=255) aatTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCa < 1:558050/133‑1 (MQ=255) tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTaaa > 1:590222/1‑133 (MQ=255) tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTaaa > 1:569505/1‑133 (MQ=255) aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTATACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg < 1:117738/133‑1 (MQ=255) ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAAt > 1:517651/1‑133 (MQ=255) ttGGTGAGATCAGGAAGAGGTTACCGAATCGAGGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAAt < 2:258733/133‑1 (MQ=255) aTCAGGAAGATGTTCACGAAACGATGGATATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa < 2:262699/133‑1 (MQ=255) tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg < 1:88441/133‑1 (MQ=255) aGGAAGAGGTTACCGAAACGATGGTCATCATCGCCCATTTTCTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg < 2:245309/133‑1 (MQ=255) ggAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggg < 1:516188/133‑1 (MQ=255) ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCTTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtatt > 1:443303/1‑133 (MQ=255) gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg < 1:227617/128‑1 (MQ=255) gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGATGGCTAAATTTAGCAAATCAATACACTTCAgggggg > 2:227617/1‑128 (MQ=255) tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTattatt > 2:603969/1‑133 (MQ=255) cGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTa < 2:626407/133‑1 (MQ=255) aCGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt < 2:603409/133‑1 (MQ=255) cGATGGACATCATCCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGtt < 2:569505/133‑1 (MQ=255) atcatcCCCCATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata < 2:590222/133‑1 (MQ=255) tcatcCCCCATTTTGTGGACTGACCC‑CCCCTGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCatat < 1:242893/133‑1 (MQ=255) ccATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa > 2:467219/1‑133 (MQ=255) cATTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt < 2:131066/133‑1 (MQ=255) aTTTTGTGGACTGCCCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg < 2:443303/133‑1 (MQ=255) | TCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCC‑CCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATG > NC_000913/2765655‑2765906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |