| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,905,219 | (C)6→8 | coding (200/672 nt) | queE ← | putative 7‑carboxy‑7‑deazaguanine synthase QueE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,905,213 | 1 | . | C | 100.0% | 147.8 / NA | 42 | A69G (GCT→GGT) | queE | putative 7‑carboxy‑7‑deazaguanine synthase QueE |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (25/17); total (25/17) | |||||||||||
| * | NC_000913 | 2,905,213 | 2 | . | C | 100.0% | 149.5 / NA | 42 | A69G (GCT→GGT) | queE | putative 7‑carboxy‑7‑deazaguanine synthase QueE |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (25/17); total (25/17) | |||||||||||
AAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑‑CCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCAT > NC_000913/2905081‑2905329 || aaaCCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAg < 2:76006/133‑1 (MQ=255)aaaCCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAg < 2:489383/133‑1 (MQ=255) cGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑‑cccc < 1:516976/133‑1 (MQ=255) tCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACGGCCTGTAAATCTTCACCGCTCTGAG‑‑ccccctc > 2:289223/1‑131 (MQ=255) tCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑‑ccccccc > 1:552936/1‑132 (MQ=255) tCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑‑ccccccc < 2:20177/133‑2 (MQ=255) tCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑‑ccccccc < 1:383390/133‑2 (MQ=255) cTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑‑cccccccc < 2:392711/133‑3 (MQ=255) gCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCa < 1:400460/133‑1 (MQ=255) gCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCa < 2:108642/133‑1 (MQ=255) cAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCAc < 2:464184/133‑1 (MQ=255) aTCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCTCGCGGTGTATCCCTGGCGACCAATGCCACACCACAAATCTTCACGGCTCGCCGCCCCCCCCACTTATCActcc > 2:504917/1‑132 (MQ=255) tcagtGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTg > 2:366108/1‑133 (MQ=255) tGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCt > 2:512988/1‑133 (MQ=255) ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCtt > 1:448227/1‑133 (MQ=255) ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCtt > 2:97709/1‑133 (MQ=255) ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCtt > 1:124704/1‑133 (MQ=255) cagcaAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCg > 2:548985/1‑133 (MQ=255) gcaAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGcc > 1:135456/1‑133 (MQ=255) caAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCa < 2:314760/133‑1 (MQ=255) aGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAgg < 1:324534/132‑1 (MQ=255) aGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAgg > 2:324534/1‑132 (MQ=255) ggCTCACCACCCGTAATCACCACATGCCGCGCGGTTTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGga < 2:135456/133‑1 (MQ=255) ggCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGga < 2:552936/133‑1 (MQ=255) caccacCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTAGCACTCTCTTTGGGCTTCGCCAGAATTCTGAGAAGGGAGACt > 2:249476/1‑133 (MQ=255) accaccCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACtt > 1:138063/1‑133 (MQ=255) caccCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTcc < 1:512988/133‑1 (MQ=255) cacaTGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCt < 1:548985/133‑1 (MQ=255) cacaTGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCt > 2:618246/1‑133 (MQ=255) aTGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCtttt < 1:366108/133‑1 (MQ=255) cgcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCa < 2:124704/133‑1 (MQ=255) gcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgt > 2:554350/1‑133 (MQ=255) cgtATCCCTGGCGACCAATGACAGCCAGCAAATATTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTACAGAGCCTCAAGTTTTTGCCAGgtgtgt > 2:291003/2‑133 (MQ=255) aTCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTg > 1:327878/1‑133 (MQ=255) ccTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgtg < 2:327878/133‑1 (MQ=255) tGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTc > 1:489545/1‑133 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGc > 2:67925/1‑133 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGc > 2:375781/1‑133 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGAGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGc > 2:500943/1‑133 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTCACAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGc > 1:420659/1‑133 (MQ=255) gCGACCAATGACAGCCAGCAAATCTTCACTGCTAGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGc > 2:539059/1‑133 (MQ=255) cGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCa < 1:97709/133‑1 (MQ=255) ccAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCa > 2:30679/1‑133 (MQ=255) cAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAg > 2:467577/1‑133 (MQ=255) tGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGGACCAGGca > 1:222152/1‑133 (MQ=255) cagccagcAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAg < 1:504917/133‑1 (MQ=255) cagcAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCaa < 1:156292/133‑1 (MQ=255) agcAAATCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAAc > 1:440406/1‑133 (MQ=255) tCTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTCGGTGTCGCACCAGGCACAGCCAACCGGGCa > 2:457405/1‑133 (MQ=255) cTTCACTGCTCGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCAt < 2:440406/133‑1 (MQ=255) cTTCACTGCTAGCAGCCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCAt < 1:539059/133‑1 (MQ=255) || AAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑‑CCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCAT > NC_000913/2905081‑2905329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |