Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,094	(G)8→9	noncoding (72/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,086	1	.	G	95.5%	67.4 / ‑3.1	22	noncoding (80/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (10/11);  total (11/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTC  >  NC_000913/4605960‑4606196                                                                                                                                |                                                                                                                 cGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑gggg‑gg                                                                                                          <  2:365616/133‑1 (MQ=255)   gCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑gggg‑gggg                                                                                                        <  1:311648/133‑1 (MQ=255)    cATTACATTTTGCTATCATCGTGCATGGGACTTATTGCTGGGATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑gggg‑ggggg                                                                                                       <  2:179656/133‑2 (MQ=255)        aCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑gggg‑gggggactt                                                                                                   >  1:338637/1‑128 (MQ=255)           ttttGCTATCATCGTACCTGGCTCTTATTGATGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGaa                                                                                                 <  1:195539/133‑1 (MQ=255)              tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAAccc                                                                                              >  1:120580/1‑133 (MQ=255)                   ccATCGTGCCTGGCACTTATTGCTGGCATCATACTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACg                                                                                         <  2:622052/133‑1 (MQ=255)                   ccATCGTGCCTGGCACTTATTGCTGGCATCATACTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACg                                                                                         >  1:622052/1‑133 (MQ=255)                         tGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGG‑GGGGACTTGAACCCCCACGTCCGt                                                                                    <  1:43241/133‑1 (MQ=255)                         tGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTa                                                                                   <  2:338637/133‑1 (MQ=255)                           ccTGGCACATATTGCAGGTATCGTGCTGATCATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAg                                                                                 <  1:247008/133‑1 (MQ=38)                                cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGAGGGCACTAGAACCCCCCCGTCGGTAAGGacac                                                                            >  2:247008/1‑133 (MQ=255)                                   ttATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                         <  2:120580/133‑1 (MQ=255)                                    tATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAac                                                                        >  1:130603/1‑133 (MQ=255)                                     aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca                                                                       >  1:125844/1‑133 (MQ=255)                                            gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                                >  1:81292/1‑133 (MQ=255)                                                                          aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                  <  1:110972/133‑1 (MQ=255)                                                                          aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                  >  2:387186/1‑133 (MQ=255)                                                                            ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCGAGCGCGTGTACGAAATCCGCCTCCTTCGct                                >  1:159459/1‑132 (MQ=21)                                                                               tGAAAAATCACGGAAAAAACAAGACAGGCAGTCAAGATAATCGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCTCAg                             <  2:159459/133‑1 (MQ=17)                                                                                     aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCt                       <  2:130603/133‑1 (MQ=255)                                                                                     aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCt                       <  2:81292/133‑1 (MQ=255)                                                                                      tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCtt                      >  2:122081/1‑133 (MQ=255)                                                                                                     gAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc       >  2:314438/1‑133 (MQ=255)                                                                                                        aGGAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGcc    <  1:122081/133‑1 (MQ=255)                                                                                                          gAAGTAAAGATAATTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTc  >  2:418952/1‑133 (MQ=255)                                                                                                                                |                                                                                                                 CGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTC  >  NC_000913/4605960‑4606196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑