Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	501,943	(C)5→6	coding (1296/1677 nt)	ybaL ←	putative transporter YbaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	501,938	1	.	C	100.0%	72.7 / NA	22	E434G (GAG→GGG)	ybaL	putative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/13);  total (9/13)

CGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCT‑CCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCT  >  NC_000913/501808‑502061                                                                                                                                    |                                                                                                                            cgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCT‑cc                                                                                                                           <  2:455984/133‑1 (MQ=255)   ccAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCT‑cccc                                                                                                                         >  2:600213/1‑133 (MQ=255)   ccAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCT‑cccc                                                                                                                         >  2:539162/1‑133 (MQ=255)   ccAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCT‑cccc                                                                                                                         >  2:316317/1‑133 (MQ=255)      gTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCT‑cccccca                                                                                                                      <  2:410668/133‑3 (MQ=255)      gTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCT‑cccccca                                                                                                                      >  1:549618/1‑131 (MQ=255)         gCATAATTACTTCGTACGACGCTTTGCCCACTACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCT‑ccccccagca                                                                                                                   <  1:290508/133‑6 (MQ=255)           aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAgg                                                                                                                  <  1:316317/133‑1 (MQ=255)                           ccgcATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGt                                                                                                  <  2:84355/133‑1 (MQ=255)                           ccgcATTGACCAATACTGCGCGTACCCCGCCCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGt                                                                                                  <  1:160337/133‑1 (MQ=255)                              cATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCGCCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAAc                                                                                               <  1:82571/133‑1 (MQ=255)                                           tGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAgcgc                                                                                  <  1:148155/133‑1 (MQ=255)                                             cgcgGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCTCCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCAt                                                                                >  2:374404/1‑133 (MQ=255)                                             cgcgGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGGCTACACGCCCGTAACCCACCAATAGCGCAt                                                                                >  1:283883/1‑133 (MQ=255)                                             cgcgGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCAt                                                                                >  1:138260/1‑133 (MQ=255)                                             cgcgGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCAt                                                                                >  2:311239/1‑133 (MQ=255)                                                  aCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTg                                                                           >  2:399631/1‑133 (MQ=255)                                                    cccGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCa                                                                         <  2:315672/133‑1 (MQ=255)                                                    cccGCGCTATCGCAGCTCGTCAGCACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCa                                                                         <  1:263247/133‑1 (MQ=255)                                                       gggcTCTCGAAGCTCTTCAACACGTGTTGGTGCCGTCTCAACCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAat                                                                      <  2:283883/131‑1 (MQ=255)                                                                                gTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGcttctcttct                                             >  1:489842/1‑133 (MQ=255)                                                                                 ttCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTcttctt                                            >  2:414202/1‑133 (MQ=255)                                                                                   cGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCg                                          >  2:378556/1‑133 (MQ=255)                                                                                    gTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGa                                         <  2:489842/133‑1 (MQ=255)                                                                                        cGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGc                                     <  1:414202/133‑1 (MQ=255)                                                                                          tctcAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCct                                   <  2:138260/133‑1 (MQ=255)                                                                                            tcAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCctct                                 >  1:47651/1‑133 (MQ=255)                                                                                                      ccaGCGGAATGTCAGCGGCGAGCAATTTCTCCCCCCAGCAGCCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCt                       <  2:162441/133‑1 (MQ=255)                                                                                                                           gCAATTTCTCCCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCt  <  2:47651/133‑1 (MQ=255)                                                                                                                                    |                                                                                                                            CGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATTTCT‑CCCCCAGCAGGCTGCCTACACGACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCT  >  NC_000913/501808‑502061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑