Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,682,4680C.20.0% 78.7 / 15.5 30coding (310/720 nt)rstADNA‑binding transcriptional regulator RstA
Reads supporting (aligned to +/- strand):  ref base C (13/11);  new base . (2/4);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACTCCCTACAAAGCCCTGCATTTCGGCACGTTGACCATCGA  >  NC_000913/1682338‑1682598
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cATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGccc                                                                                                                                  >  2:141016/1‑133 (MQ=255)
   gACCATTTGTCGTGATTTCCGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCct                                                                                                                               <  1:211237/133‑1 (MQ=255)
               tGATTTACGCACAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACG‑CCCCTGCTGTTTTGCTAg                                                                                                                  >  1:608185/1‑133 (MQ=255)
                                      gACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACG‑CCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGt                                                                                           <  1:79311/133‑1 (MQ=255)
                                           aTTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCGTGCTGTTTTGCTCGAGGGTTTACGTGTGCATGTGCGTCAGa                                                                                       >  2:290410/1‑133 (MQ=255)
                                           aTTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGa                                                                                       >  2:4805/1‑133 (MQ=255)
                                           aTTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGa                                                                                       >  2:131634/1‑133 (MQ=255)
                                           aTTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACG‑CCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGaa                                                                                      >  2:18027/1‑133 (MQ=255)
                                                    ctAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAg                                                                              <  1:63781/133‑1 (MQ=255)
                                                       aCCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCca                                                                           >  2:534919/1‑133 (MQ=255)
                                                         ctctctCGCTAGCGATATCAACGACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCcaca                                                                         <  1:258141/133‑1 (MQ=255)
                                                            tctcGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAGAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTg                                                                      >  1:530890/1‑133 (MQ=255)
                                                                gATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCa                                                                  <  1:534919/133‑1 (MQ=255)
                                                                gATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACG‑CCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCaa                                                                 <  2:608185/133‑1 (MQ=255)
                                                                   aGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAg                                                               >  1:145393/1‑133 (MQ=255)
                                                                   aGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAg                                                               >  1:425751/1‑133 (MQ=255)
                                                                     cGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGTGTCAGAAAGAGCAAGCGACACTGACCAAAGGt                                                             >  2:172371/1‑133 (MQ=255)
                                                                      gATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTCTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTc                                                            >  1:589124/1‑133 (MQ=255)
                                                                             aCCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGa                                                     <  2:145393/133‑1 (MQ=255)
                                                                                  aTCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCGAATGTCTTCTGGAAACGt                                                >  1:230635/1‑133 (MQ=255)
                                                                                      tGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTctct                                            <  1:131634/133‑1 (MQ=255)
                                                                                        gCACTGGATATGGGTGCCTGCGTCTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGa                                          <  1:202962/133‑1 (MQ=255)
                                                                                         cACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACG‑CCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACt                                        <  1:141016/133‑1 (MQ=255)
                                                                                           cTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACTc                                       <  2:589124/133‑1 (MQ=255)
                                                                                            tGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACTcc                                      >  2:332296/1‑133 (MQ=255)
                                                                                              gAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACTCCCt                                    >  1:463369/1‑133 (MQ=255)
                                                                                                aaTGGGTGCCTGCGACTATATTCTCAAAACGACG‑CCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACTCCCTACa                                 <  1:18027/133‑1 (MQ=255)
                                                                                                                  tatTCTCAGAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACTCCCTACAAAGCCCTGCATTTCGGc                <  2:530890/133‑1 (MQ=255)
                                                                                                                  tatTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACTCCCTACAAAGCCCTGCATTTCGGc                <  1:332296/133‑1 (MQ=255)
                                                                                                                             cgacgCCCACTGCTGCTTCGCTCGCGCGTTGACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACTCCCTACAAAGCCCTGCATTTCGGCACGTTGACCAt     <  1:172371/133‑1 (MQ=255)
                                                                                                                                cgCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACTCCCTACAAAGCCCTGCATTTCGGCACGTTGACCATCGa  >  1:612071/1‑133 (MQ=255)
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CATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGACTCCCTACAAAGCCCTGCATTTCGGCACGTTGACCATCGA  >  NC_000913/1682338‑1682598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑