Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,635,322	C→T	E176K (GAA→AAA)	ydfN ←	Qin prophage; putative side tail fiber assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,635,322	0	C	T	100.0%	25.9 / NA	14	E176K (GAA→AAA)	ydfN	Qin prophage; putative side tail fiber assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/10);  total (4/10)

TTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCA  >  NC_000913/1635216‑1635446                                                                                                           |                                                                                                                             ttGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGTACTGGATgcgc                                                                                                    >  2:106159/1‑133 (MQ=17)  tgtgTTGGTGCTCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGTGCCTCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCTAAATCTGTACTGGATgcgct                                                                                                   <  2:104447/133‑1 (MQ=17)   gtgtTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGTACTGGATgcgctg                                                                                                  >  1:89694/1‑133 (MQ=17)      ttGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGTACTGGATgcgctggcg                                                                                               <  1:38077/133‑1 (MQ=17)          tGCGCACCCGCACTATTTCCGGACCCGCTATTACTATGGGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGTACTGGATGCGCTGGCGCtgt                                                                                           <  1:195910/133‑1 (MQ=11)                 cGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGTACTGGATGCGCTGGCGCTGTGGGtgtg                                                                                    <  1:619440/133‑1 (MQ=14)                           tGCGGTCCCGCTAATACTATGGGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATg                                                                          >  2:606828/1‑133 (MQ=11)                                                                            ttagtgccGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCt                         >  2:556901/7‑133 (MQ=2)                                                                                         cAAACGACGAAGTGGCTTTCGTCCTCAAATCTCTACTGGATTCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGcc            <  2:175179/133‑1 (MQ=11)                                                                                           agcgacgaTGGGGTTCTCGTCCCCGAATGTGTGCTGGACGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCTCGACCACGGGCGGGCTTGCCCTTAATCGTCCAGCCAc          <  2:229946/131‑1 (MQ=11)                                                                                            atgacgaTGTAGTTTTCGTCCCCTTAACAGTACTGGATGCGGTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACg         <  2:304631/131‑1 (MQ=11)                                                                                            acgacgATGTGGTTTTCGTCCCCAAATCTGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACg         <  1:606828/133‑1 (MQ=255)                                                                                            acgacgATGTGGTTTTCGTCCCCAAATCTGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACg         <  2:68425/133‑1 (MQ=255)                                                                                                   tgtgGTTTTCGTCCCCAAATCTGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCa  <  1:556901/133‑1 (MQ=255)                                                                                                   tgtgGTTTTCGTCCCCAAATCTGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCa  <  2:581857/133‑1 (MQ=255)                                                                                                           |                                                                                                                             TTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGTACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCA  >  NC_000913/1635216‑1635446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑