Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,666,361 A→G E349E (GAA→GAG)  clcB → putative chloride:H(+) antiporter ClcB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,666,3610AG97.0% 111.7 / NA 33E349E (GAA→GAG) clcBputative chloride:H(+) antiporter ClcB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (19/13);  minor base T (0/1);  total (19/14)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATT  >  NC_000913/1666235‑1666490
                                                                                                                              |                                                                                                                                 
tgCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTaca                                                                                                                             >  2:479321/1‑133 (MQ=255)
 gCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTacac                                                                                                                            >  2:81944/1‑133 (MQ=255)
     tGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACtttt                                                                                                                        >  2:70688/1‑133 (MQ=255)
      gCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTa                                                                                                                       >  1:572832/1‑133 (MQ=255)
      gCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTACACCGATGGCGAGGAGATTACACTTTTa                                                                                                                       >  2:287261/1‑133 (MQ=255)
                    gTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGAc                                                                                                            >  2:557834/1‑130 (MQ=255)
                    gTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGAc                                                                                                            <  1:557834/130‑1 (MQ=255)
                          gcgcACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTGTACGCGAATTGACGGGGATGGc                                                                                                   >  1:189800/1‑133 (MQ=255)
                                            ttACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGAc                                                                                 <  2:311428/133‑1 (MQ=255)
                                                     cGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGTTTGACCGGGATGGCGAAACTGTTGGTGGCGACCacgcacgc                                                                        >  2:274243/1‑133 (MQ=255)
                                                     cGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc                                                                        <  2:485708/133‑1 (MQ=255)
                                                           ttATCGGTCTTGCCACTGCCATCTTGTATGCTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGAt                                                                  <  1:170678/133‑1 (MQ=255)
                                                                  tCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCg                                                           <  1:41604/133‑1 (MQ=255)
                                                                        cATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTtg                                                     <  1:70688/133‑1 (MQ=255)
                                                                          ttGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTtgat                                                   <  2:99140/133‑1 (MQ=255)
                                                                          ttGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTtgat                                                   <  2:421105/133‑1 (MQ=255)
                                                                          ttGGCATGGTGTATGGTCGTAGGCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTtgat                                                   <  1:274243/133‑1 (MQ=255)
                                                                            ggCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTtgatga                                                 >  2:418507/1‑133 (MQ=255)
                                                                                   tgtATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGa                                          <  2:551222/133‑1 (MQ=255)
                                                                                     tATGGTCGTAACCTGTGATTATGGCTCCTTGATGGCGAAGATATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGaaa                                        <  2:201992/133‑1 (MQ=255)
                                                                                        ggTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGa                                     >  1:410364/1‑133 (MQ=255)
                                                                                            gTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCgg                                 >  2:542657/1‑133 (MQ=255)
                                                                                             tAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCggg                                >  1:551220/1‑133 (MQ=255)
                                                                                             tAGCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCggg                                >  2:79026/1‑133 (MQ=255)
                                                                                               gCCTGGGATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCTCCGATTATGTCGGCGTTTATGATATGTGAAGTGACCGGGGa                              >  2:286232/1‑133 (MQ=255)
                                                                                                 cTGGGATTATGGTTCCCTCATGGCGAAGAGTTTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGt                            <  1:248078/133‑1 (MQ=255)
                                                                                                   gggATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAt                          <  2:541188/133‑1 (MQ=255)
                                                                                                    ggATTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGAGTATGTCGACGTTGATGGTATGTGAATTGACCGGGGAGTATc                         >  2:248078/1‑133 (MQ=255)
                                                                                                      aTTATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAg                       <  1:81944/133‑1 (MQ=255)
                                                                                                       ttATGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTAGATGATATGTGAAATGGCCGGGGAGTATCAGc                      >  2:227041/1‑133 (MQ=255)
                                                                                                         aTGGTTCCCTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTa                    >  2:109477/1‑133 (MQ=255)
                                                                                                               cccTGATGGCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGGGAAATGAGCGGGGAGTATCAGATACTcccc              >  1:228256/1‑133 (MQ=255)
                                                                                                                   gATGGCGACGAGATTGCAGTTTGACTCGGAGTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGtt          <  1:287261/133‑1 (MQ=255)
                                                                                                                      ggCGAAGAGATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTat       >  1:555808/1‑133 (MQ=255)
                                                                                                                         gaagagATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattga    <  1:79026/133‑1 (MQ=255)
                                                                                                                           agagATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgatt  <  1:418507/133‑1 (MQ=255)
                                                                                                                              |                                                                                                                                 
TGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATT  >  NC_000913/1666235‑1666490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑