Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,730,251	A→G	I389V (ATT→GTT)	lhr →	putative ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,730,251	0	A	G	100.0%	107.3 / NA	30	I389V (ATT→GTT)	lhr	putative ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/18);  total (12/18)

ACGTTTTTATTGCCCGCTCACACCACGGCTCCGTCTCTAAAGAACAACGAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGATTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGTA  >  NC_000913/1730129‑1730380                                                                                                                           |                                                                                                                                  aCGTTTTTATAGCCCTCTCACACCACGGGTCCTTCTCTAAAGAACAACGAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATggg                                                                                                                         <  2:221309/133‑1 (MQ=255)    tttttATTGCCCGCTCACACCACGGCTCCGTCTCTAAAGAACAACGAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGgcgc                                                                                                                      >  1:621625/1‑133 (MQ=255)      tttATTGCCCCCTCACACCACGGCTCCGTCTCGAAAGAACAACGAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCgg                                                                                                                    <  2:254506/133‑1 (MQ=255)                                  tctcTAAAGAACAACGAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGTAAcgcc                                                                                        >  1:472275/1‑133 (MQ=255)                                  tctcTAAAGAACAACGAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAAcgcc                                                                                        >  1:510562/1‑133 (MQ=255)                                              aacGAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGc                                                                            <  2:510562/133‑1 (MQ=255)                                                cGAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCa                                                                          <  2:381286/133‑1 (MQ=255)                                                cGAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCa                                                                          <  1:578854/133‑1 (MQ=255)                                                 gAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAg                                                                         <  2:9436/133‑1 (MQ=255)                                                 gAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAg                                                                         <  2:9339/133‑1 (MQ=255)                                                 gAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAg                                                                         <  1:92882/133‑1 (MQ=255)                                                        cACCGAACGGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTa                                                                  <  1:298962/133‑1 (MQ=255)                                                           cGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACaa                                                               >  2:465359/1‑133 (MQ=255)                                                           cGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACaa                                                               >  2:400879/1‑133 (MQ=255)                                                               cAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCa                                                           <  1:139302/133‑1 (MQ=255)                                                                  gcgcTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTg                                                        >  2:62197/1‑133 (MQ=255)                                                                            cGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCgg                                              >  2:402123/1‑133 (MQ=255)                                                                                tGAATTACGCAGCGTAGTCGCAACATACAGTCTTGAACTGGTGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACAt                                          <  2:272573/133‑1 (MQ=255)                                                                                         cTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGc                                 >  2:141737/1‑133 (MQ=255)                                                                                          tGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGTAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCg                                <  2:472275/133‑1 (MQ=255)                                                                                          tGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAAGGCATTGGACGCGCCAGACATCAGGTTGGCg                                >  1:182780/1‑133 (MQ=255)                                                                                          tGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCg                                <  1:101349/133‑1 (MQ=255)                                                                                            cGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTgg                                  >  2:97332/1‑129 (MQ=255)                                                                                            cGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTgg                                  <  1:97332/129‑1 (MQ=255)                                                                                            cGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGgt                              <  1:402123/133‑1 (MQ=255)                                                                                            cGTGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGgt                              >  1:400678/1‑133 (MQ=255)                                                                                              tGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTgg                                  >  2:500882/1‑127 (MQ=255)                                                                                              tGGTCGCAACCTCCAGTCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTgg                                  <  1:500882/127‑1 (MQ=255)                                                                                                         tCCAGTCTTGCACTGCGGGTTGATCTGGCCGCCGTGGATCTCGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGc                 <  2:182780/133‑1 (MQ=255)                                                                                                              tCTTGAACTGGGGGTTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGttt            <  2:615254/133‑1 (MQ=255)                                                                                                                        ggggTTGATATGGGCGCGGTGGATCTGGTGATTCAGGGGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGTa  >  1:41027/1‑133 (MQ=255)                                                                                                                           |                                                                                                                                  ACGTTTTTATTGCCCGCTCACACCACGGCTCCGTCTCTAAAGAACAACGAGCAATCACCGAACAGGCGCTGAAATCGGGTGAATTACGCTGCGTGGTCGCAACCTCCAGTCTTGAACTGGGGATTGATATGGGCGCGGTGGATCTGGTGATTCAGGTGGCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGTA  >  NC_000913/1730129‑1730380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑