Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,747,140	A→C	I4L (ATT→CTT)	ydhS →	FAD/NAD(P) binding domain‑containing protein YdhS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,747,140	0	A	C	100.0%	126.3 / NA	36	I4L (ATT→CTT)	ydhS	FAD/NAD(P) binding domain‑containing protein YdhS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/17);  total (19/17)

AGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGAT  >  NC_000913/1747019‑1747268                                                                                                                          |                                                                                                                                 aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATtgtg                                                                                                                       >  1:152098/1‑133 (MQ=255) aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATtgtg                                                                                                                       >  1:440128/1‑133 (MQ=255)      ggTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGc                                                                                                                  <  2:440128/133‑1 (MQ=255)      ggTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGc                                                                                                                  <  1:382717/133‑1 (MQ=255)              cccTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTa                                                                                                          >  2:26361/1‑133 (MQ=255)               ccTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTAc                                                                                                         >  2:106156/1‑133 (MQ=255)               ccTTTTCATCCCTGTCTGAAATTTCTCAAATTATAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTAc                                                                                                         >  2:415359/1‑133 (MQ=255)                cTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACg                                                                                                        <  2:626066/133‑1 (MQ=255)                cTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACg                                                                                                        <  2:316280/133‑1 (MQ=255)                             tCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCt                                                                                           >  1:6770/1‑133 (MQ=255)                             tCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCt                                                                                           >  1:465585/1‑133 (MQ=255)                               tGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTa                                                                                         >  2:89850/1‑133 (MQ=255)                                 aaaTTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTAtt                                                                                       <  1:26361/133‑1 (MQ=255)                                      tctcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGc                                                                                  >  2:444823/1‑133 (MQ=255)                                       ctcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCt                                                                                 >  1:46016/1‑133 (MQ=255)                                       ctcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCt                                                                                 <  2:142698/133‑1 (MQ=255)                                         cAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTc                                                                               <  1:444823/133‑1 (MQ=255)                                           aaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTa                                                                             >  2:538578/1‑133 (MQ=255)                                                tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGca                                                                        <  1:538578/133‑1 (MQ=255)                                                tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGca                                                                        <  2:68787/133‑1 (MQ=255)                                                tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGca                                                                        <  2:152098/133‑1 (MQ=255)                                                   aaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGcaaca                                                                     <  2:6770/133‑1 (MQ=255)                                                          aaCCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCa                                                              <  1:415359/133‑1 (MQ=255)                                                                  ttATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctat                                                      <  1:106156/133‑1 (MQ=255)                                                                     tCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatttc                                                   <  2:46016/133‑1 (MQ=255)                                                                         aTAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATc                                               <  1:89850/133‑1 (MQ=255)                                                                            acacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGGGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAACAACATAATCCACTTTCTATTCCTATCTTc                                            >  1:299620/1‑133 (MQ=255)                                                                                   aatCGAAAGCTTGCGTACAGGTAAGTAAGAATAAAAAAACTAGCTATTGTGGGTGCCGAGCCTACGGAGATCTACAACATATTCTCGCTTCTACAGCAACAAACTCCACTTTCAACTTCTATCTTCGAGCAgg                                     <  2:299620/131‑1 (MQ=255)                                                                                   aTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTAAGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAgg                                     >  1:391805/1‑133 (MQ=255)                                                                                                     aGGTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCg                   <  2:38310/133‑1 (MQ=255)                                                                                                      ggTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCgg                  >  1:553434/1‑133 (MQ=255)                                                                                                      ggTAAGTAACAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCCTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCgg                  >  2:350101/1‑133 (MQ=255)                                                                                                       gtaagtaaCAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCCTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCggg                 <  1:350101/133‑1 (MQ=255)                                                                                                           gtaaCAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGc             >  2:468028/1‑133 (MQ=255)                                                                                                               cAATGAAAGAACTTGATACTGTAGGTGCCGCGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATa         <  1:291434/133‑1 (MQ=255)                                                                                                               cAATGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATa         >  1:143290/1‑133 (MQ=255)                                                                                                                 aTGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACa       >  1:41998/1‑133 (MQ=255)                                                                                                                 aTGAAAAAACTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGCCTGACGAGGCCGGTATCGGGACGCCATACa       >  1:160488/1‑133 (MQ=255)                                                                                                                      aaaacTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgat  >  2:525489/1‑133 (MQ=255)                                                                                                                      aaaacTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCAAGCAGGCTGACGGGGCTGGTGTCGGGATGCCTTACAGtggt  >  2:291434/1‑131 (MQ=255)                                                                                                                          |                                                                                                                                 AGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGAT  >  NC_000913/1747019‑1747268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑