Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,762,442	A→G	V24A (GTT→GCT)	sufC ←	Fe‑S cluster scaffold complex subunit SufC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,762,442	0	A	G	100.0%	120.1 / NA	34	V24A (GTT→GCT)	sufC	Fe‑S cluster scaffold complex subunit SufC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/28);  total (6/28)

TCTTTGCCTTTGAACTCAACCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCT  >  NC_000913/1762316‑1762533                                                                                                                               |                                                                                            tCTTTGCCTTTGAACTCAACCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGa                                                                                       >  1:17838/1‑133 (MQ=255)    ttGCCTTTGAACTCAACCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGc                                                                                    >  1:148128/1‑133 (MQ=255)         tttGAACTCAACCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAAt                                                                               <  2:17838/133‑1 (MQ=255)           tGAACTCAACCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATcc                                                                             >  2:352002/1‑133 (MQ=255)               cgcAACCGTCCCGCCCGTCACTTACTAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCgcgc                                                                         <  2:293243/131‑1 (MQ=255)               cTCAACCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGTTGAGCGTCGAGGCTTAATCCgcgc                                                                         <  2:415271/133‑1 (MQ=255)                  aaCCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAgg                                                                      <  2:571467/133‑1 (MQ=255)                   aCCGTGCCTCCCGTCGCTGCATTATCTTCTAGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGa                                                                     <  1:295297/133‑1 (MQ=255)                   aCCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGa                                                                     <  2:371844/133‑1 (MQ=255)                   aCCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCACATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGa                                                                     <  2:395404/133‑1 (MQ=255)                     cGTGCCGCCTCTCACGTCATAATCTTGTGGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATa                                                                   <  2:272432/133‑1 (MQ=255)                         ccgccCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCcgcg                                                                          >  2:222497/1‑122 (MQ=255)                         ccgccCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCcgcg                                                                          <  1:222497/122‑1 (MQ=255)                                 cACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTcc                                                       <  1:461185/133‑1 (MQ=255)                                    ttCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACg                                                    <  2:445773/133‑1 (MQ=255)                                              tctcGCCCGGCTAGCCTTGCCGATTAGGCACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTa                                          <  2:227566/133‑1 (MQ=255)                                              tctcGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGTAGGATAGCTTTATCTTCCACg                                                    >  2:579491/1‑123 (MQ=255)                                              tctcGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGTAGGATAGCTTTATCTTCCACg                                                    <  1:579491/123‑1 (MQ=255)                                              tctcGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTa                                          >  1:480189/1‑133 (MQ=255)                                               ctcGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTaa                                         <  1:352002/133‑1 (MQ=255)                                               ctcGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTaa                                         <  2:419515/133‑1 (MQ=255)                                               ctcGCCCGGCAAGCGGTGCCGATACGGTGCTTCTGCCCGAATCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTCTATCTTCCACGCTGACGTGTaa                                         <  2:192717/133‑1 (MQ=255)                                                 cGCCCGGCAAGCGTTTCCTATACGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAAt                                       <  1:168180/133‑1 (MQ=255)                                                 cGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAAt                                       <  2:148128/133‑1 (MQ=255)                                                    ccGGCAAGCGTTGCCGATCAGGCACTTTTGACCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCtt                                    <  1:169176/133‑1 (MQ=255)                                                    ccGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCtt                                    <  1:11564/133‑1 (MQ=255)                                                       gCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTaa                                 <  2:620653/133‑1 (MQ=255)                                                              ttGCCGATAAGGTACTTTCGCCTGAACCGTATGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTaa                          <  1:199295/133‑1 (MQ=255)                                                               tGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAAc                         <  1:307971/133‑1 (MQ=255)                                                               tGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAAc                         <  1:110340/133‑1 (MQ=255)                                                                     tAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGttt                   <  1:616766/133‑1 (MQ=255)                                                                                      aaCCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCt  <  2:386179/133‑1 (MQ=255)                                                                                      aaCCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCt  <  2:480189/133‑1 (MQ=255)                                                                                      aaCCGTTTGGCCCCACAATGGCGTGAACTTCGCCGGGATGAGCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCt  <  1:280227/133‑1 (MQ=255)                                                                                                                               |                                                                                            TCTTTGCCTTTGAACTCAACCGTGCCGCCCGTCACTTCATAATCTTCTCGCCCGGCAAGCGTTGCCGATAAGGTACTTTTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCT  >  NC_000913/1762316‑1762533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑