Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,828,768	A→G	S323P (TCT→CCT)	astD ←	aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,828,768	0	A	G	100.0%	32.6 / NA	11	S323P (TCT→CCT)	astD	aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/1);  total (10/1)

AGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCG  >  NC_000913/1828662‑1828898                                                                                                           |                                                                                                                                   aGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGGAATGAGCGCGACAATAAGCGGCTGCg                                                                                                          >  1:303437/1‑133 (MQ=255) aGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTAAGGAATCAGCTCGCCAATAAGCGGCTGCg                                                                                                          >  1:305985/1‑133 (MQ=255)  ccAACGATGCTCCTGATTGTAATACGCGCGGCCCAAGCAGGTGTCGCCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTACCCCCCTGCTGTGCGGCCTGTTCAGGAACCAGCCCGCCAATAAACGGCTGCgg                                                                                                         <  2:187577/132‑1 (MQ=255)            cccTGCTTGACATAACCGGGGGGATAGCCGGGGTCGCCCGCTCATCGCTTCCGGTTGCTCCCATGCAGTAACCCCCTGTTGTGCGGCCTGTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATcc                                                                                               <  2:259748/133‑1 (MQ=255)                gCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGGAATCAGCCCGACAATAAACGGCAGCAGTTCGTGATCCCAGt                                                                                           >  1:289342/1‑133 (MQ=255)                    gTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGcc                                                                                       <  1:427074/133‑1 (MQ=255)                    gTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCAGGCAGTAACCCCCTGCTGTGCGCCCTGTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGcc                                                                                       <  2:392073/133‑1 (MQ=255)                                gcgcAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCg                                                                           >  1:84080/1‑133 (MQ=255)                                                tcCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAAcc                                                           >  2:491346/1‑133 (MQ=255)                                                                          aTGCAGTACCCCCCTCCTGTGCGGTTTGTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTg                                 <  2:289342/133‑1 (MQ=255)                                                                                  accaccTGCTGTGCGGCCTGTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCACACGCGCACTAAACGCATCGCCCTGCGccccac                         >  2:302615/1‑131 (MQ=255)                                                                                          ctgTGCGGCCTGTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGc                 >  1:63357/1‑133 (MQ=255)                                                                                              gCGGCCTGTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACCCATCGCCATGCGCCTCGCTTTTCAGCAATa             >  1:272762/1‑133 (MQ=255)                                                                                                ggCCTGTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGCTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATaaa           >  1:610309/1‑133 (MQ=255)                                                                                                     gTTCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAAcggcg      >  1:12366/1‑133 (MQ=255)                                                                                                       tCAGGAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACggcggg    <  1:556430/133‑1 (MQ=255)                                                                                                         aGAAACCACCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACggcgggcg  <  2:12366/133‑1 (MQ=255)                                                                                                           |                                                                                                                                   AGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCG  >  NC_000913/1828662‑1828898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑