Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,853,832	T→C	S50G (AGC→GGC)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,853,832	0	T	C	100.0%	114.5 / NA	33	S50G (AGC→GGC)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/19);  total (14/19)

TGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCG  >  NC_000913/1853706‑1853956                                                                                                                               |                                                                                                                             tGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTAc                                                                                                                           <  2:432708/130‑1 (MQ=255) tGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTAc                                                                                                                           >  1:432708/1‑130 (MQ=255)           ccTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAg                                                                                                              >  1:350525/1‑133 (MQ=255)             tGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAgac                                                                                                            <  1:359074/133‑1 (MQ=255)                    cccAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTa                                                                                                     >  1:259847/1‑133 (MQ=255)                           tagtCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGt                                                                                              <  1:122491/133‑1 (MQ=255)                              tCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAAc                                                                                           >  1:565109/1‑133 (MQ=255)                                       aaCCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAAcc                                                                                  <  1:96636/133‑1 (MQ=255)                                       aaCCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAAcc                                                                                  <  2:346700/133‑1 (MQ=255)                                       aaCCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAAcc                                                                                  <  2:259847/133‑1 (MQ=255)                                       aaCCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAAcc                                                                                  >  2:143607/1‑133 (MQ=255)                                       aaCCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAAcc                                                                                  <  2:138112/133‑1 (MQ=255)                                         cccACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCt                                                                                <  2:350525/133‑1 (MQ=255)                                            accaGTAAGTGGGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGtt                                                                             >  1:612938/1‑133 (MQ=255)                                            accaGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTACTAAGACGACATTACCGGAGTAACTCAAAAACCCTGtt                                                                             >  2:111201/1‑133 (MQ=255)                                                 tAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTtaataa                                                                        <  2:361117/133‑1 (MQ=255)                                                        gCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTa                                                                 <  2:565109/133‑1 (MQ=255)                                                        gCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTa                                                                 <  2:52609/133‑1 (MQ=255)                                                           gATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAg                                                              >  1:326411/1‑133 (MQ=255)                                                                  tAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTataa                                                       <  2:326411/133‑1 (MQ=255)                                                                      cAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATAcc                                                   <  1:407664/133‑1 (MQ=255)                                                                      cAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACCCAAAAACCATGTTAATGACAGACGAAAGCTCATAATAcc                                                   >  1:172329/1‑133 (MQ=255)                                                                               aTGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAat                                               <  1:562174/128‑1 (MQ=255)                                                                               aTGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAat                                               >  2:562174/1‑128 (MQ=255)                                                                                     aGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATg                                    >  2:482354/1‑133 (MQ=255)                                                                                                 cATTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCaa                        >  1:559771/1‑133 (MQ=255)                                                                                                  aTTGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCaaa                       >  2:421646/1‑133 (MQ=255)                                                                                                   ttGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAg                      <  2:612938/133‑1 (MQ=255)                                                                                                   ttGAGGAAATTATTTGACCATCCATTGCCTACCAGCTTTACTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAg                      <  1:111201/133‑1 (MQ=255)                                                                                                        gAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAg                 <  1:143607/133‑1 (MQ=255)                                                                                                        gAAATTATTTGACCATCCATTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAg                 <  1:482354/133‑1 (MQ=255)                                                                                                        gAAATTATTTAACCACCCATTGCCTACCTGCTCTGCTAAGATGACATTACCGGAGTAACCCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAg                 <  2:172329/133‑1 (MQ=255)                                                                                                                       tccatTGCCTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAAGCGGGCCAAAGGGAAGCGGTCCGGTCTTgcg  >  1:169307/1‑133 (MQ=255)                                                                                                                               |                                                                                                                             TGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCG  >  NC_000913/1853706‑1853956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑