Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 202,482 A→C K128Q (AAA→CAA)  fabZ → 3‑hydroxy‑acyl‑[acyl‑carrier‑protein] dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913202,4820AC100.0% 131.5 / NA 37K128Q (AAA→CAA) fabZ3‑hydroxy‑acyl‑[acyl‑carrier‑protein] dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/18);  total (19/18)

GGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGATACGTGATTGATAAATCCGCCTTTGTGCATCCAACCGC  >  NC_000913/202361‑202594
                                                                                                                         |                                                                                                                
gggTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGct                                                                                                       <  2:404046/133‑1 (MQ=255)
gggTGAGCTGTACTACTTCGCTGGAATTCACGCAGCGCGCTACAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGct                                                                                                       <  2:178733/133‑1 (MQ=255)
  gTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGctct                                                                                                     >  1:456210/1‑133 (MQ=255)
   tGAGCTGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTg                                                                                                    >  2:541/1‑133 (MQ=255)
        tGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGa                                                                                               <  2:425128/133‑1 (MQ=255)
            ctactTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGt                                                                                           <  2:147297/133‑1 (MQ=255)
               ctTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTaaa                                                                                        >  2:120855/1‑133 (MQ=255)
               ctTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCAGCCTGACCGGTTGTCAAGGGGTTGCCCTGGGCGATGGTaaa                                                                                        >  2:191681/1‑133 (MQ=255)
                 tCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAagt                                                                                      >  1:387528/1‑133 (MQ=255)
                    cTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCGAGGGGTTACTCAGGACGATGGCAAagtagt                                                                                   >  1:292297/1‑133 (MQ=255)
                    cTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAagtagt                                                                                   >  1:461894/1‑133 (MQ=255)
                    cTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAagtagt                                                                                   >  1:576218/1‑133 (MQ=255)
                    cTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTGCAAAGGGATGCTCTGGACGATGGTAAagtagt                                                                                   >  2:216413/1‑133 (MQ=255)
                       gTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTg                                                                                >  2:315561/1‑133 (MQ=255)
                         aTTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGACCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCg                                                                              <  1:337823/133‑1 (MQ=255)
                            gACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAg                                                                           <  2:387528/133‑1 (MQ=255)
                            gACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAg                                                                           <  1:56682/133‑1 (MQ=255)
                                   cgcgcTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACgat                                                                    <  1:342147/133‑1 (MQ=255)
                                       cTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGatgatg                                                                <  2:576218/133‑1 (MQ=255)
                                                  cGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAg                                                     >  2:129906/1‑133 (MQ=255)
                                                   ggTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGc                                                    <  1:315561/133‑1 (MQ=255)
                                                      cctGCCTGGCGATCAGATGATCATAGAATTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCgg                                                 <  2:292297/131‑1 (MQ=255)
                                                       gTGCCTGGCGATCAACTGATCATGAAAGCCACTTTCGAAAACACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCggg                                                <  2:178892/133‑1 (MQ=255)
                                                        tGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGa                                               <  1:129906/133‑1 (MQ=255)
                                                            tGGCGATCAAATGATCATGGAATTCACTTTTGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGcc                                           <  1:216413/133‑1 (MQ=255)
                                                               cGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGa                                        >  1:375059/1‑133 (MQ=255)
                                                               cGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGa                                        >  1:308275/1‑133 (MQ=255)
                                                               cGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTATGCGCGAAGCCGGTAGGCCTGa                                        >  1:178892/1‑133 (MQ=255)
                                                                gATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGAt                                       <  2:555577/133‑1 (MQ=255)
                                                                   cAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGACCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGATACg                                    <  2:563823/133‑1 (MQ=255)
                                                                           cATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGCTGATGTGTTCTCTTAGCCGGGAGGCCTGATAAGtgattgat                            >  2:278011/1‑133 (MQ=255)
                                                                            aTGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGATACGTGATTGATa                           <  2:63055/133‑1 (MQ=255)
                                                                              ggAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGATACGTGATTGATaaa                         >  2:591181/1‑133 (MQ=255)
                                                                                   tCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGATACGTGATTGATAAATCCGc                    >  1:138778/1‑133 (MQ=255)
                                                                                   tCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGATACGTGATTGATAAATCCGc                    >  1:80851/1‑133 (MQ=255)
                                                                                         tCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGATACGTGATTGATAAATCCGCCTTtgt              <  1:541/133‑1 (MQ=255)
                                                                                         tCGAAAAAACGCGCCGCGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGATACGTGATTGATAAATCCGCCTTtgt              >  1:371180/1‑133 (MQ=255)
                                                                                                     gccgcGGCCTGACCCGTTTTCAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGATACGTGATTGATAAATCCGCCTTTGTGCATCCAACCGc  <  2:80851/133‑1 (MQ=255)
                                                                                                     gccgcGGCCTGACCCGTTTTAAGGGGGTTGCTTGGGTGGATGGTAAAGTAGTTTGCGAAGCACCGTTGATGTGTGCTCGTAGCGGGGAGGCCTGATACGTGATTGATAAATCCGCTTTTGTGCATCCAACCGc  <  2:138778/133‑1 (MQ=255)
                                                                                                                         |                                                                                                                
GGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTCGTAGCCGGGAGGCCTGATACGTGATTGATAAATCCGCCTTTGTGCATCCAACCGC  >  NC_000913/202361‑202594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑