Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,366	T→C	R78R (CGT→CGC)	yoaC →	DUF1889 domain‑containing protein YoaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,366	0	T	C	100.0%	74.8 / NA	22	R78R (CGT→CGC)	yoaC	DUF1889 domain‑containing protein YoaC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/16);  total (6/16)

AATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGACGCTTTTAATCACATAT  >  NC_000913/1894236‑1894481                                                                                                                                   |                                                                                                                    aaTATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTc                                                                                                                   >  1:45596/1‑133 (MQ=255)                   gAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGC‑AAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGaa                                                                                                <  2:460131/133‑1 (MQ=255)                            cgAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTc                                                                                        <  1:338193/133‑1 (MQ=255)                            cgAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTc                                                                                        <  2:412031/133‑1 (MQ=255)                                          atTACGGCGCGAACTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAgg                                                                          <  1:409734/133‑1 (MQ=255)                                           ttACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGga                                                                         <  2:45596/133‑1 (MQ=255)                                              cGGCGCGAGCCGACCCGGAAGGCTTGAACCCTCGGTTCACGCAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGgaaga                                                                      <  2:174326/133‑1 (MQ=255)                                                gcgcGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAAc                                                                    >  2:330462/1‑133 (MQ=255)                                                   cgAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTaa                                                                 <  2:356607/133‑1 (MQ=255)                                                      gCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAg                                                              >  1:491568/1‑133 (MQ=255)                                                        tGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCa                                                            <  2:126711/133‑1 (MQ=255)                                                         gACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTGCAACATAGATGGAGGAAGAACGAAAAGCAc                                                           >  1:170515/1‑133 (MQ=255)                                                             aGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGt                                                       <  1:458556/133‑1 (MQ=255)                                                                    cTGGAACCCTGGGTTCATGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGtt                                                <  2:129840/133‑1 (MQ=255)                                                                    cTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGtt                                                <  2:349657/133‑1 (MQ=255)                                                                         aCCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAAtt                                           >  2:446080/1‑133 (MQ=255)                                                                              gggTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTatat                                      <  2:405648/133‑1 (MQ=255)                                                                                                 ttGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGa                   <  2:491568/133‑1 (MQ=255)                                                                                                 ttGGATGGGCAAAAAAAATGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGa                   <  1:446080/133‑1 (MQ=255)                                                                                                           aaaaaaaaTGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGACGCTTTTAAt         <  1:321583/133‑1 (MQ=255)                                                                                                             aaaaaaTGGACACAGTTGAACGCTATGTCATTTAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGACGCTTTTAATca       <  2:170515/133‑1 (MQ=255)                                                                                                                  aTGGAGACAGGTGAACGCTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGACGCTTTTAATCACatat  >  2:128294/1‑133 (MQ=255)                                                                                                                                   |                                                                                                                    AATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGACGCTTTTAATCACATAT  >  NC_000913/1894236‑1894481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑