Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,942,858	A→C	K66T (AAA→ACA)	znuC →	Zn(2(+)) ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,942,858	0	A	C	100.0%	115.4 / NA	32	K66T (AAA→ACA)	znuC	Zn(2(+)) ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/15);  total (17/15)

GTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTC  >  NC_000913/1942730‑1942985                                                                                                                                 |                                                                                                                                gtCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTg                                                                                                                             >  2:395566/1‑133 (MQ=255)      tGGAACTTGAACCTGGAAAAATGTTGACTTCACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCAt                                                                                                                        <  1:201966/133‑1 (MQ=255)              aaaCCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATg                                                                                                                <  1:560986/133‑1 (MQ=255)                   tGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCg                                                                                                           >  2:543915/1‑133 (MQ=255)                   tGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCg                                                                                                           <  1:602424/133‑1 (MQ=255)                    ggAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGc                                                                                                          <  2:350021/133‑1 (MQ=255)                          aTTTTGACGTTACTTAGGTCAAGTGGCGCATGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGc                                                                                                    <  1:291146/133‑1 (MQ=255)                                       ttGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGacac                                                                                       <  1:508880/133‑1 (MQ=255)                                                   gcgcAGGTCAGTCGAAACTGGTACGGGTAGGGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACt                                                                           <  1:251402/133‑1 (MQ=255)                                                     gcAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCAc                                                                            <  2:583694/130‑1 (MQ=255)                                                     gcAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCAc                                                                            >  1:583694/1‑130 (MQ=255)                                                      cAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCACAAGGTATCTCTCGACACCACGTTGCCACTgcc                                                                        >  2:279333/1‑131 (MQ=255)                                                                       gTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGttttt                                                          >  1:143946/1‑130 (MQ=255)                                                                       gTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGttttt                                                          <  2:143946/130‑1 (MQ=255)                                                                       gTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttac                                                       >  2:61610/1‑133 (MQ=255)                                                                          cGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgct                                                    <  2:422333/133‑1 (MQ=255)                                                                            ggTAGTGCTCGGGCTGGTTACACCCGATGAGGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgctta                                                  <  1:297761/133‑1 (MQ=255)                                                                            ggTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgctta                                                  <  2:502005/133‑1 (MQ=255)                                                                             gTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgcttac                                                 >  1:481677/1‑133 (MQ=255)                                                                              tAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgcttacg                                                <  1:61610/133‑1 (MQ=255)                                                                                gTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGcc                                              <  1:395566/133‑1 (MQ=255)                                                                                 tGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGccc                                             >  1:366821/1‑133 (MQ=255)                                                                                 tGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGccc                                             >  1:141369/1‑133 (MQ=255)                                                                                                  cccGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCATTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAaagaag                            >  1:4752/1‑133 (MQ=255)                                                                                                  cccGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGGAAACCGTTTTTTGCGCTTACGCCCTGGTACACATAaagaag                            >  2:192627/1‑133 (MQ=255)                                                                                                  cccGATGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGCAAACCGTTGTTTGCGCCTACGCCCTGGTACGCATAaagaag                            >  2:172219/1‑133 (MQ=255)                                                                                                      aTGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGatat                        >  2:100869/1‑133 (MQ=255)                                                                                                      aTGAAGGGGTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCACTGATAGACATAAGGAAGatat                        >  1:186572/1‑133 (MQ=255)                                                                                                           ggggTTATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCATTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGc                   <  2:4752/133‑1 (MQ=255)                                                                                                               ttATCAAGCGCAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGc               >  1:46222/1‑133 (MQ=255)                                                                                                                      gcgcAACGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGaaa        >  2:141022/1‑133 (MQ=255)                                                                                                                            cGGAACACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTc  >  2:562867/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                                GTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTC  >  NC_000913/1942730‑1942985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑