Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,945,078 C→T K93K (AAG→AAA)  ruvB ← Holliday junction branch migration complex subunit RuvB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,945,0780CT97.0% 103.3 / ‑3.9 33K93K (AAG→AAA) ruvBHolliday junction branch migration complex subunit RuvB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (18/14);  total (19/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGC  >  NC_000913/1944955‑1945207
                                                                                                                           |                                                                                                                                 
ttGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGc                                                                                                                          <  2:494920/133‑1 (MQ=255)
 tGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCAAAACCGACCGCTTATTCCAGCa                                                                                                                         >  1:237575/1‑133 (MQ=255)
  ggTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCAc                                                                                                                        >  1:520837/1‑133 (MQ=255)
     aGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCgg                                                                                                                     >  2:567370/1‑133 (MQ=255)
     aGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCgg                                                                                                                     >  2:386238/1‑133 (MQ=255)
               tGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTc                                                                                                           <  2:387239/133‑1 (MQ=255)
                           cACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCATCACCCGACCAGACGTCGCTCGTAAAtga                                                                                               >  2:300031/1‑131 (MQ=255)
                                     acaacTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCAttt                                                                                     >  2:507239/1‑133 (MQ=255)
                                     acaacTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCAttt                                                                                     >  1:324201/1‑133 (MQ=255)
                                      caacTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTc                                                                                    >  2:320016/1‑133 (MQ=255)
                                        acTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAAAAGTCGTGCGTCAAGTAACGCCCATTTCAt                                                                                  >  1:224557/1‑133 (MQ=255)
                                          tGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCCTAACTTCACGCCCCTTTCATTg                                                                                >  1:204921/1‑133 (MQ=255)
                                              gATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGa                                                                            <  1:386238/133‑1 (MQ=255)
                                                tAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACa                                                                          >  1:379620/1‑133 (MQ=255)
                                                 aGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACaa                                                                         >  1:519493/1‑133 (MQ=255)
                                                 aGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGAGTAAATTCACGACCGTTTCATAGGCGACaa                                                                         >  1:186552/1‑133 (MQ=255)
                                                      gTGGATCTCATCAATTAACAGCACATCATGCGGTTCAAGGTCAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTg                                                                    <  2:179186/133‑1 (MQ=255)
                                                       tGGATCTCTTCAATAAACGGCAAGTCATGCTGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTgg                                                                   <  1:300031/133‑1 (MQ=255)
                                                       tGGATCTCATAAATAAACAGCACGTCATCCGGTTCAAGGTTGGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTgg                                                                   <  2:237575/133‑1 (MQ=255)
                                                        ggATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGc                                                                  <  1:559614/133‑1 (MQ=255)
                                                               atcaATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTa                                                           >  1:379873/1‑133 (MQ=255)
                                                                  aaTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGtt                                                        <  2:379620/133‑1 (MQ=255)
                                                                   aTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGttt                                                       <  1:507239/133‑1 (MQ=255)
                                                                   aTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGttt                                                       >  1:455431/1‑133 (MQ=255)
                                                                    tAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCg                                                             <  1:510015/126‑1 (MQ=255)
                                                                    tAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCg                                                             >  2:510015/1‑126 (MQ=255)
                                                                           cACGTCATGCGGTTCAAGGGTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAAc                                               >  2:405639/1‑133 (MQ=255)
                                                                                          aaGGTTAGTGCGCATTGCAGCCAACTCGGCCGATTTTTCCACCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCaaaa                                <  2:224557/133‑1 (MQ=255)
                                                                                            ggTTAGTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAAt                              <  2:324201/133‑1 (MQ=255)
                                                                                            ggTTAGTGAGAATCGTAGCCAAATTGCCCGCTCTTTCCAGCGCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAAt                              <  2:204921/133‑1 (MQ=255)
                                                                                                 gTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATcaaca                         >  2:392670/1‑133 (MQ=255)
                                                                                                 gTGAGCATCGCAGCCAAATCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGAAAGCGTAGATTTACCCAACCCCGGATGACCGAAAATcaaca                         >  2:184273/1‑133 (MQ=255)
                                                                                                                   tCGCCCGCTTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATcgc       <  2:455431/133‑1 (MQ=255)
                                                                                                                        cgctTTTTCCGGCACTGGACCAGATGTCCTGCTTAAATTAACGCCCATTTCATTGGCGACAATGTTAGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCgcgc  <  2:186552/133‑1 (MQ=255)
                                                                                                                        cgctTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCgcgc  <  2:519493/133‑1 (MQ=255)
                                                                                                                           |                                                                                                                                 
TTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGC  >  NC_000913/1944955‑1945207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑