| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,968,611 | G→T | L252I (CTT→ATT) | cheR ← | chemotaxis protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,968,611 | 0 | G | T | 96.2% | 83.2 / NA | 26 | L252I (CTT→ATT) | cheR | chemotaxis protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (11/14); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGC > NC_000913/1968482‑1968740 | atcacCCTGGTTTTGATCATCGTTTATCCTTACTTAGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCGGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAg < 2:281425/131‑1 (MQ=255)aaCACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAg < 2:566770/133‑1 (MQ=255)aaCACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAg < 2:327885/133‑1 (MQ=255) ccTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGaa < 2:530955/133‑1 (MQ=255) gATTTTGCTCATCGTTAATCCTTACTTAGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCGGAGTGACCCGCAAACGGCAATCCGTCGGGTTTAATGAGCGGAACaa > 1:281425/1‑133 (MQ=255) gATTTTGCTCATCGTTAATCCTTACTTAGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACaa > 2:136121/1‑133 (MQ=255) ttttGCTCATCGTTAATCCTTACTTAGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAg < 2:430000/133‑1 (MQ=255) tCGTTAATCCTTACTTAGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGAGGCACAAAGCGGCGCaaa > 1:234288/1‑133 (MQ=255) cttacttaGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCctgc > 1:376714/1‑133 (MQ=255) cgcATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGtt > 2:449931/1‑133 (MQ=255) cgcATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGtt > 2:432083/1‑133 (MQ=255) cgcATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGtt > 2:393294/1‑133 (MQ=255) cgcATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGtt > 1:537718/1‑133 (MQ=255) tACACCGTCTTACCACGCAGCGTGAAGCAGCGCTCAAGGTGACTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGAt < 2:158799/133‑1 (MQ=255) acacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATc > 1:445592/1‑133 (MQ=255) gTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGt < 2:537718/133‑1 (MQ=255) cTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAg > 1:488577/1‑133 (MQ=255) tGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGa > 2:58050/1‑133 (MQ=255) aGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGt < 1:58050/133‑1 (MQ=255) cGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTa < 2:445592/133‑1 (MQ=255) ggCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCaaa < 1:393294/133‑1 (MQ=255) cAGAGTGAGCCGCAAGCATCAATCCGTCGGTTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCAc < 1:276489/133‑1 (MQ=255) cAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCAc < 1:136121/133‑1 (MQ=255) cAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCAc < 1:377258/133‑1 (MQ=255) agagTGACCCGCAAACAGCAATCCGTCGGGTTTAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACg < 2:488577/133‑1 (MQ=255) tAATGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGc > 1:509826/1‑133 (MQ=255) | AACACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGC > NC_000913/1968482‑1968740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |