Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	205,071	A→G	A193A (GCA→GCG)	rnhB →	RNase HII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	205,071	0	A	G	100.0%	64.9 / NA	19	A193A (GCA→GCG)	rnhB	RNase HII
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

GATATTGTTTTCCCGCAATATGGTTTTGCCCAACACAAAGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTTCGTACACCTGCGGGTGCACAGCGACTACTCGATGATCGATGGCCTGGCCA  >  NC_000913/204943‑205192                                                                                                                                 |                                                                                                                          gATATTGTTTTCCCGCAATATGGTTTTGCCCACCACAAAGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTgg                                                                                                                       <  2:207143/133‑1 (MQ=255)           tCCCGCAATATGGTTTTGCCCAACACAAAGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTc                                                                                                             >  1:1260/1‑133 (MQ=255)            cccGCAATATGGTTTTGCCCAACACAAAGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTcc                                                                                                            >  2:43586/1‑133 (MQ=255)               gCAATATGGTTTTGCCCAACACAAAGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGa                                                                                                         <  1:14502/133‑1 (MQ=255)                  atatGGTTTTGCCCAACACAAAGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTc                                                                                                      >  1:477306/1‑133 (MQ=255)                        ttttGCCCAACACAAAGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTgtgt                                                                                                <  2:477306/133‑1 (MQ=255)                             cccAACACAAAGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCgaga                                                                                           >  1:518726/1‑133 (MQ=255)                                       aGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAAc                                                                                 >  1:390996/1‑133 (MQ=255)                                        gggTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAAcc                                                                                <  1:490509/133‑1 (MQ=255)                                                  ccGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGaa                                                                      >  1:134857/1‑133 (MQ=255)                                                   cGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAg                                                                     >  1:591868/1‑133 (MQ=255)                                                                       tGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTTCGt                                                 >  1:475979/1‑133 (MQ=255)                                                                          cTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATTTGAAGATGTCTGAACCGCGTTTCGTaca                                              >  2:220485/1‑133 (MQ=255)                                                                                   gcgcGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTTCGTACACCTGCGGGt                                     <  2:591868/133‑1 (MQ=255)                                                                                          cGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTTCGTACACCTGCGGGTGCACAGc                              <  2:390996/133‑1 (MQ=255)                                                                                                 cATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTTCGTACACCTGCGGGTGCACAGCGactact                       <  1:43586/133‑1 (MQ=255)                                                                                                 cATCGGCGCAGCTTTGGGCCTGTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTTCGTACACCTGCGGGTGCACAGCGactact                       <  2:475979/133‑1 (MQ=255)                                                                                                                    cTGTCAAATGCGCGCAGGCACTCGCGTCCTAATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTTCGTACACCTGCGGGTGCACAGCGACTACTCGATGATCGAtggcctggc    <  1:291459/133‑1 (MQ=255)                                                                                                                      gTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTTCGTACACCTGCGGGTGCACAGCGACTACTCGATGATCGATGGCCTGGCCa  >  1:517478/1‑133 (MQ=255)                                                                                                                      gTCAAACGCGCGCTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTTCGTACACCGGCGGGTGCACAGTGACATCTCGATGATCGAttgcctggcaa  >  2:291459/1‑131 (MQ=255)                                                                                                                                 |                                                                                                                          GATATTGTTTTCCCGCAATATGGTTTTGCCCAACACAAAGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACCGAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGATTCTTGTGTCGAGATTAAGTAAACCGGAATCTGAAGATGTCTGAACCACGTTTCGTACACCTGCGGGTGCACAGCGACTACTCGATGATCGATGGCCTGGCCA  >  NC_000913/204943‑205192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑