Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,066,253	A→G	intergenic (‑489/+52)	cobU ← / ← insH‑6	cobinamide‑P guanylyltransferase/cobinamide kinase/CP4‑44 prophage; IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,066,253	0	A	G	94.7%	36.5 / ‑4.1	19	intergenic (‑489/+52)	cobU/insH‑6	cobinamide‑P guanylyltransferase/cobinamide kinase/CP4‑44 prophage; IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (10/8);  total (10/9)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAG  >  NC_000913/2066126‑2066301                                                                                                                                |                                                 gAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAg                                             <  1:527703/133‑1 (MQ=255)  aaTTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGc                                            <  2:567141/133‑1 (MQ=255)    ttATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCt                                          >  2:569746/1‑133 (MQ=255)         cGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTa                                     >  2:15842/1‑133 (MQ=255)                gtggCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGtt                              >  1:337627/1‑133 (MQ=255)                      aCTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTCg                        <  2:337627/133‑1 (MQ=255)                            aattAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTAGCCAttt                  >  2:407127/4‑133 (MQ=0)                                 gggAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGc             >  1:429504/1‑133 (MQ=17)                                 gggAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGc             >  1:558747/1‑133 (MQ=18)                                  ggAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTAATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg            >  2:308076/1‑133 (MQ=18)                                    aaGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGtt          >  1:494019/1‑133 (MQ=17)                                         gCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATccc     <  1:83432/133‑1 (MQ=17)                                          cGAATAAGTGGGGAACTTATTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcccc    <  1:295300/133‑1 (MQ=17)                                          cGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcccc    <  1:387908/133‑1 (MQ=17)                                          cGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTAGCCATTTAAGGCGTTATcccc    <  1:407127/133‑1 (MQ=17)                                           gAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCa   <  2:558747/133‑1 (MQ=17)                                           gAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAAACCGTTTCTTCGCCATTTAAGGCGTTATcc      >  1:43605/1‑130 (MQ=17)                                           gAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAAACCGTTTCTTCGCCATTTAAGGCGTTATcc      <  2:43605/130‑1 (MQ=12)                                            aaTAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGGATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAg  >  2:24874/1‑133 (MQ=17)                                                                                                                                |                                                 GAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAG  >  NC_000913/2066126‑2066301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑