Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,090,243	A→C	S18R (AGT→CGT)	hisG →	ATP phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,090,243	0	A	C	100.0%	85.7 / NA	25	S18R (AGT→CGT)	hisG	ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/11);  total (14/11)

TCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTC  >  NC_000913/2090129‑2090372                                                                                                                   |                                                                                                                                  tCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAAt                                                                                                                 <  1:357707/133‑1 (MQ=255)      gggCTTTTTTATTGCGCGGTTGATGACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTg                                                                                                            >  1:124994/1‑133 (MQ=255)         cTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGgcg                                                                                                         <  1:332305/133‑1 (MQ=255)               tATTTCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCtgt                                                                                                   <  1:516446/133‑1 (MQ=255)                   gcgcGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCTTTTACGTGGTGACTCACGCGAATTGGTGGCCCGCTGTggta                                                                                               >  2:287329/1‑131 (MQ=255)                    cgcgGTTGATGACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCAt                                                                                              <  2:124994/133‑1 (MQ=255)                    cgcgGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCAt                                                                                              >  2:16168/1‑133 (MQ=255)                           gATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAAtt                                                                                       <  2:123059/133‑1 (MQ=255)                                                      gAATAACAAAGTGACCGACAACACTCGTCTACCCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATcgcg                                                            <  2:179863/133‑1 (MQ=255)                                                      gAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATcgcg                                                            >  2:112586/1‑133 (MQ=255)                                                      gAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATcgcg                                                            >  1:324418/1‑133 (MQ=255)                                                      gAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAAATAACCTTCACACCCAGCGGCTGATcgcg                                                            >  2:284784/1‑133 (MQ=255)                                                                 tGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGaaaa                                                 >  1:534043/1‑133 (MQ=255)                                                                     agacaACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATg                                             <  2:534043/133‑1 (MQ=255)                                                                       acaacaCTCTTTTACACGTAACGATGCGGAAGTCGGGCGGGGGACATCATCACTCACACAAATTCCTCTCACCCTTTCGCATTAAAATTAATCTTCACACCGACCCCCTTATCCCAATCTCAGAAAACATGcc                                           <  2:434238/133‑1 (MQ=255)                                                                       acaacaCTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCCCGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCTGCGCCTGATCGCGATCGCAGAAAACATGcc                                           <  2:389463/133‑1 (MQ=255)                                                                       acaacaCTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGTCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGcc                                           >  2:528100/1‑133 (MQ=255)                                                                                 tttACGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATAtt                                 >  1:505778/1‑133 (MQ=255)                                                                                     cGCATAGCTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTgc                             >  1:580394/1‑133 (MQ=255)                                                                                            cTATGCAGAAATCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCgcgtgcg                      >  1:42025/1‑133 (MQ=255)                                                                                            cTATGCAGAAATCCGCCCGTTTCCGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCgcgtgcg                      <  1:284784/133‑1 (MQ=255)                                                                                                    aaaTCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTAATCGCGATGGCAGAAAACATGCCGATTGATATTGTGAGCGTGCCTgacgacg              >  1:203670/1‑133 (MQ=255)                                                                                                      aTCCGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTgacgacgac            >  1:135458/1‑133 (MQ=255)                                                                                                         cGGCCGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACAtt         <  1:594034/133‑1 (MQ=255)                                                                                                           gCCGTTTACGTGATGTCTGACGAGAATTGCGGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTcc       <  1:286654/133‑1 (MQ=255)                                                                                                            ccGTTTACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTccc      >  1:91948/1‑133 (MQ=255)                                                                                                                ttACGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATTGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTc  <  1:528100/133‑1 (MQ=255)                                                                                                                   |                                                                                                                                  TCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTC  >  NC_000913/2090129‑2090372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑