Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,146	T→C	N61S (AAC→AGC)	galF ←	UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,146	0	T	C	100.0%	143.2 / NA	40	N61S (AAC→AGC)	galF	UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/24);  total (16/24)

GCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCA  >  NC_000913/2114021‑2114271                                                                                                                              |                                                                                                                              gCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGa                                                                                                                        <  1:552529/133‑1 (MQ=255)      ttCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGt                                                                                                                   <  1:491732/133‑1 (MQ=255)           aaTGGTCAGGCCCGGCGGGCAGATGGACTGGACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGtt                                                                                                              <  1:194646/133‑1 (MQ=255)               gTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCa                                                                                                          >  1:545618/1‑133 (MQ=255)               gTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCa                                                                                                          >  1:428272/1‑133 (MQ=255)                 cACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCagg                                                                                                        >  2:403306/1‑133 (MQ=255)                 cACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTACGAACGCGCTCTTGGAAGCGTGAGTTACCagg                                                                                                        >  2:294762/1‑133 (MQ=255)                   cGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCACGCACTTGGACGTGTGAGTTACCaggag                                                                                                      >  2:250386/1‑133 (MQ=255)                     cccGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAggagga                                                                                                    <  2:582919/133‑1 (MQ=255)                      ccGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGAt                                                                                                   <  2:568557/133‑1 (MQ=255)                       cggcggACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGAtt                                                                                                  <  2:569210/133‑1 (MQ=255)                       cggcggACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGAtt                                                                                                  <  2:345405/133‑1 (MQ=255)                              cAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTGCCTGGAGGAGTTCTTTGa                                                                                           >  1:261180/1‑133 (MQ=255)                                         tACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGcc                                                                                >  2:26170/1‑133 (MQ=255)                                              ccGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAAt                                                                           >  1:156419/1‑133 (MQ=255)                                                   agcagTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGt                                                                      <  2:545618/133‑1 (MQ=255)                                                   agcagTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGt                                                                      <  2:386349/133‑1 (MQ=255)                                                   agcagTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGt                                                                      >  1:523134/1‑133 (MQ=255)                                                        ttGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTcaaca                                                                 <  2:84024/133‑1 (MQ=255)                                                        ttGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTcaaca                                                                 <  2:428272/133‑1 (MQ=255)                                                         tGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTcaacaa                                                                <  1:35047/133‑1 (MQ=255)                                                                         gctCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATTCCTGCAGCTACAATCTTGTCAACAAGGTACCGAATCATTgg                                                >  2:216683/1‑133 (MQ=255)                                                                          ctCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGc                                               <  1:130273/133‑1 (MQ=255)                                                                           tCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCt                                              >  2:307612/1‑133 (MQ=255)                                                                              aGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGt                                           <  1:366954/133‑1 (MQ=255)                                                                                 aGTGATTCTAACTCACAAAAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTcga                                        <  1:250386/133‑1 (MQ=255)                                                                                    gATTCTAACTCATAAGAGTTGTTGAAGTGGGTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTcgacga                                     <  1:216683/133‑1 (MQ=255)                                                                                    gATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTcgacga                                     >  1:4819/1‑133 (MQ=255)                                                                                     aTTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGAt                                    <  2:523134/133‑1 (MQ=255)                                                                                         tAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGt                                <  2:156419/133‑1 (MQ=255)                                                                                           aCTCATAAGAGGAGTCGAAGTGGTTTTCGTCCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCATCAACAATATACAGAAGCATTGGCTTGTCGGCGATTGGTAg                              >  2:164032/1‑133 (MQ=255)                                                                                            cTCATAAGTGGTGTCGAACTGGTTTTCGACTGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc                             <  1:294762/133‑1 (MQ=255)                                                                                            cTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc                             <  1:403306/133‑1 (MQ=255)                                                                                            cTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc                             <  2:517483/133‑1 (MQ=255)                                                                                                   gagGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCtt                      >  1:566469/1‑133 (MQ=255)                                                                                                   gagGTGTCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCtt                      <  2:135056/133‑1 (MQ=255)                                                                                                       tgtCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg                  >  1:522196/1‑133 (MQ=255)                                                                                                        gtCGAAGTGGTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGt                 <  1:26170/133‑1 (MQ=255)                                                                                                                 gTTTTCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGAGTGGGAGCATCTCGTTGGCTATCGCCTTa        >  2:277610/1‑133 (MQ=255)                                                                                                                   tttCGACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGt      >  1:126906/1‑133 (MQ=255)                                                                                                                       gACCGCGCTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTggca  <  2:4819/133‑1 (MQ=255)                                                                                                                              |                                                                                                                              GCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCA  >  NC_000913/2114021‑2114271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑