| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,152,194 | A→G | R93R (CGT→CGC) | pphC ← | protein‑serine/threonine phosphatase PphC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,152,194 | 0 | A | G | 96.2% | 84.3 / ‑4.0 | 26 | R93R (CGT→CGC) | pphC | protein‑serine/threonine phosphatase PphC |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (11/14); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
TACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGACATATAAGCCATCGCTTCATTGACGGCGAGCATCGCGCC > NC_000913/2152065‑2152304 | tACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGa < 2:79973/133‑1 (MQ=255)tACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGa < 2:131141/133‑1 (MQ=255) ccacGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGACGAATGGt < 1:46172/133‑1 (MQ=255) cacGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGt < 2:508112/132‑1 (MQ=255) cacGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGt > 1:508112/1‑132 (MQ=255) acgtccccATCCCCAAGCCGAATAATCCAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCa < 2:576818/127‑1 (MQ=255) cgccgcCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGt < 1:498196/130‑1 (MQ=255) cgccgcCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGt > 2:498196/1‑130 (MQ=255) tCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGc < 2:571338/133‑1 (MQ=255) gCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAg < 1:36442/133‑1 (MQ=255) ggTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTa > 1:392121/1‑133 (MQ=255) gTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTaa > 2:626305/1‑133 (MQ=255) cGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGccc < 1:385091/133‑1 (MQ=255) cGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGccc > 2:526606/1‑133 (MQ=255) gTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGcccc > 1:407431/1‑133 (MQ=255) aaTGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCg < 2:392121/133‑1 (MQ=255) gtgCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGACa > 1:387465/1‑133 (MQ=255) gCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGACata > 2:315900/1‑133 (MQ=255) cAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCg > 2:315422/1‑127 (MQ=255) cAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCg < 1:315422/127‑1 (MQ=255) aGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGACAtata > 2:570137/1‑133 (MQ=255) aCCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGACATATAAGCCATCGCTTCAt < 2:548994/133‑1 (MQ=255) tAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGACATATAAGCCATCGCTTCATTGACg < 2:387465/133‑1 (MQ=255) tCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGACATATAAGCCATCGCTTCATTGACGGCGAGCATCgcgc < 1:626305/133‑1 (MQ=255) tCCGCCTCGGCAAAAAGTCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGACATATAAGCCATCGCTTCATTGACGGCGAGCATCgcgc < 1:315900/133‑1 (MQ=255) ccGCCTCGGCAAAAAGGCGTTGGCGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGACATATAAGCCATCGCTTCATTGACGGCGAGCATCGCGcc > 1:484313/1‑133 (MQ=255) | TACCACCACGCCGCCATCGCCAATCTGCATAATCAAGGTGCCGTCAGGCGATGAAATCAGTCCCAGAAATGTGCAGGCAAAATCGCGAACCGCTAATTCTTTGGCTTCCGCCTCGGCAAAAAGTCGTTGACGAATGGTCAGTACCATATTTGTGGCGAGGACATCATTAAGCCCCAATTCCCCGCCCTGCACTTTTTGCGACATATAAGCCATCGCTTCATTGACGGCGAGCATCGCGCC > NC_000913/2152065‑2152304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |