Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,268,972 A→G Y40C (TAT→TGT)  lpxT → Kdo2‑lipid A phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,268,9720AG100.0% 124.2 / NA 35Y40C (TAT→TGT) lpxTKdo2‑lipid A phosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/17);  total (18/17)

AGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTT  >  NC_000913/2268845‑2269088
                                                                                                                               |                                                                                                                    
agagAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTTGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCt                                                                                                                 >  1:543394/1‑133 (MQ=255)
agagAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCt                                                                                                                 >  1:61038/1‑133 (MQ=255)
 gagaAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCtt                                                                                                                <  2:371798/133‑1 (MQ=255)
    aaaaTATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTaa                                                                                                             >  1:587770/1‑133 (MQ=255)
       atatGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTATTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGAGGATATTTTTTGTTTCTTTAAtta                                                                                                          >  2:303827/1‑131 (MQ=255)
            aTTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCtt                                                                                                                >  2:66507/1‑122 (MQ=255)
            aTTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCtt                                                                                                                <  1:66507/122‑1 (MQ=255)
            aTTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAAc                                                                                                     >  1:137552/1‑133 (MQ=255)
                                 gtgtTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATTTCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCtttt                                                                                <  1:20589/133‑1 (MQ=255)
                                     tgttgAATATTGTTGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTtgtg                                                                            <  2:543394/133‑1 (MQ=255)
                                     tgttgAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTtgtg                                                                            >  1:512484/1‑133 (MQ=255)
                                     tgttgAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTtgtg                                                                            <  2:110862/133‑1 (MQ=255)
                                       ttgAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTTAGGCCTTTTTGTGGc                                                                          >  1:267221/1‑133 (MQ=255)
                                       ttgAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTtgtg                                                                            <  1:608979/131‑1 (MQ=255)
                                       ttgAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTtgtg                                                                            >  2:608979/1‑131 (MQ=255)
                                       ttgAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGc                                                                          >  1:428297/1‑133 (MQ=255)
                                       ttgAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTACCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGc                                                                          >  1:48770/1‑133 (MQ=255)
                                               tGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGTATATTTTTTGTTTCTTTAATCAGAAACTGGGCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCa                                                                  >  2:336597/1‑133 (MQ=255)
                                               tGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCa                                                                  <  2:137552/133‑1 (MQ=255)
                                               tGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCa                                                                  <  2:309871/133‑1 (MQ=255)
                                               tGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGATCGAACGACAGGCCTTGTTGTTGCTGGTTgga                                                                  >  2:286137/1‑131 (MQ=255)
                                                gTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCAt                                                                 <  2:587770/133‑1 (MQ=255)
                                                gTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCAt                                                                 <  2:428297/133‑1 (MQ=255)
                                                 tCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCAtt                                                                >  1:384140/1‑133 (MQ=255)
                                                 tCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCAtt                                                                >  1:83202/1‑133 (MQ=255)
                                                                    ttcCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTc                                             <  1:32868/133‑1 (MQ=255)
                                                                      ccTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGa                                           <  2:387479/133‑1 (MQ=255)
                                                                      ccTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGa                                           <  2:35529/133‑1 (MQ=255)
                                                                         ggTATATCGCCGTTAGTCGTGGGTTCTGGTAGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACgg                                        <  1:303827/133‑1 (MQ=255)
                                                                                    gTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCActgc                             >  1:66989/1‑133 (MQ=255)
                                                                                    gTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCActgc                             >  2:551150/1‑133 (MQ=255)
                                                                                    gTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCActgc                             >  1:594177/1‑133 (MQ=255)
                                                                                        aTCATGGATTCTGGTTACCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGc                         <  2:48770/133‑1 (MQ=255)
                                                                                         tCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCg                        <  2:267221/133‑1 (MQ=255)
                                                                                                        gCCGATTGATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATg         <  2:512484/133‑1 (MQ=255)
                                                                                                               gATGCGGATATTTTTTGTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGtt  >  2:619504/1‑133 (MQ=255)
                                                                                                                               |                                                                                                                    
AGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTT  >  NC_000913/2268845‑2269088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑