Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,433,011	A→G	intergenic (‑69/+1)	folC ← / ← accD	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase/acetyl‑CoA carboxyltransferase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,433,011	0	A	G	100.0%	31.7 / NA	10	intergenic (‑69/+1)	folC/accD	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase/acetyl‑CoA carboxyltransferase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/5);  total (5/5)

CCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGAC  >  NC_000913/2432882‑2433139                                                                                                                                  |                                                                                                                                 ccAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagttgtca                                                                                                                               >  1:43668/1‑133 (MQ=255)   aGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTGTCAgg                                                                                                                             <  2:43668/133‑1 (MQ=255)    gATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTGTCAGGc                                                                                                                            <  2:415105/133‑1 (MQ=255)              ccACGAAGCCAGAGGCGACGCGGCTTGACGAGTGCGTTTGACAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTGTCAGGCCTCAGGTTcc                                                                                                                  <  2:262088/133‑1 (MQ=255)                          agGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTGTCAGGCCTCAGGTTCCTGATCCGGTAcc                                                                                                      <  2:605900/133‑1 (MQ=255)                                                      aTAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTGTCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACgcggcg                                                                          >  2:524884/1‑133 (MQ=255)                                                         aTCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTGTCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCtt                                                                       <  1:16949/133‑1 (MQ=255)                                                                       tGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTGTCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCt                                                         >  1:97315/1‑133 (MQ=255)                                                                                                  gggCAGAGCCAGTGGCCCTGCCCTTATCAGTTGTCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATg                              >  1:366974/1‑133 (MQ=255)                                                                                                  gggCAGAGCCAGTGGCCCTGCCCTTATCAGTTGTCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGACAGATTCATCAACTTCGCCAGAATg                              >  2:11522/1‑133 (MQ=255)                                                                                                                    tgccctTATCAGTTGTCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgcgc            >  1:86504/1‑133 (MQ=255)                                                                                                                           atcagttgtcagGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCcg     <  2:473751/133‑1 (MQ=255)                                                                                                                              agttgtcagGCCTCAGGTTCCTGATCCGGTACCGGGGGGACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCcggac  >  1:110943/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                                 CCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGAC  >  NC_000913/2432882‑2433139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑