Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,880	T→C	intergenic (+234/‑132)	fadL → / → yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,880	0	T	C	100.0%	23.7 / NA	8	intergenic (+234/‑132)	fadL/yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/5);  total (3/5)

GTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑A‑‑‑CTCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATAT  >  NC_000913/2462756‑2462975                                                                                                                                      |                                                                                                  gTTGGCAGCTAGCCCTTTCAAAACAATAAACGGTATCCCCAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCTCCTCCTTCTCAAAAAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑A‑‑‑cccccccccc                                                                                           <  2:231831/133‑1 (MQ=255)      cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑A‑‑‑Cccccccccccctgg                                                                                      <  1:620082/133‑5 (MQ=255)        gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑A‑‑‑cccccccc                                                                                             >  2:623322/1‑124 (MQ=255)        gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑A‑‑‑cccccccc                                                                                             <  1:623322/124‑1 (MQ=255)        gCTTGCCCTTTCAAAACAAAAAGGGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCCCGTCCTTCTCAAAGAGTTTTCTTTTCTGCATTTCCA‑G‑GA‑T‑‑A‑‑‑Ccccccccccccctggc                                                                                    <  2:437524/133‑7 (MQ=255)              ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GATT‑‑C‑‑‑CCCCCCCCCCC‑‑TGGCTATtgtg                                                                             <  1:412138/133‑1 (MQ=255)                ttttAAATCAATAAGCGGTTTCCCCAGGAAAACCTTAAGGGAAAGGGATTTTTTCAGGGG‑TTTCGCCCGTCCCTCTCAAAGGGTTTTCTTTTTTTCCTTTCCAGG‑GA‑TCCC‑‑‑CCCCCCCCCCC‑‑TGGCTATtgtg                                                                             <  2:69958/133‑1 (MQ=255)                                  tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑ACC‑CCCCCCCCCCC‑‑TGGCTTTGGTGCGCACTTACAGTCAAATTa                                                          >  1:253216/1‑133 (MQ=255)                                  tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑A‑‑‑CCCCCCCCCCCCGTGGCTATTGTGGCCTCATACCTTCAAATTa                                                          >  1:218733/1‑133 (MQ=255)                                     ccACAGGCAAACCTGAAGGGAAAGGCATATTTTCGGGGG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCG‑G‑GA‑T‑‑G‑‑‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                     <  2:588249/133‑1 (MQ=255)                                          gACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑ACC‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                  >  2:431304/1‑133 (MQ=255)                                            cAAACCCGAAGGGAAACGGATATTTTGCAGCG‑TTTTTCTCGTCCTTCCCAAAGAGGTTTCTTTTATGGCTTTTCA‑G‑GG‑T‑‑ACC‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                <  2:396488/133‑1 (MQ=255)                                                         aaaGGCATATTTTCAGGCG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑AC‑‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGc                                  >  2:467078/1‑133 (MQ=255)                                                         aaaGGAATTTTTTCATGCGTTTCTG‑TCCATCTGCTCAAAAAGTTTTCTTTTCTGCATTTCCA‑G‑GA‑TTCC‑‑‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                   <  2:253216/133‑1 (MQ=255)                                                                                ttcTCGTCCTTCTCAAAAAGTTTTTTTTTCTGCATTTCCA‑GAGT‑TC‑C‑‑‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa             <  2:245039/131‑1 (MQ=255)                                                                                tGCTCGTCCTTCTCAAAGAATTTTTTTTTTTTCATTTCCA‑G‑AA‑T‑‑ACC‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa             <  2:218733/133‑1 (MQ=255)                                                                                  cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑ACC‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt           <  2:355792/133‑1 (MQ=255)                                                                                   tCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑ACC‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa          <  1:428329/133‑1 (MQ=255)                                                                                     gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑ACC‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg        <  1:431304/133‑1 (MQ=255)                                                                                      tCCTTCTCCAAGAGTCTTCTTTCCTGCATTACCA‑G‑GA‑T‑‑ACCCCCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg        <  1:159894/133‑1 (MQ=255)                                                                                           ccaaaaGATTTTTCTTTTTTTCATTTCCA‑G‑AA‑TCCC‑‑‑CCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat  <  2:149211/130‑1 (MQ=255)                                                                                                                                      |                                                                                                  GTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCG‑TTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCA‑G‑GA‑T‑‑A‑‑‑CTCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATAT  >  NC_000913/2462756‑2462975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑