Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	256,406	G→C	V144L (GTA→CTA)	gpt →	xanthine‑guanine phsophoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	256,406	0	G	C	100.0%	105.8 / NA	31	V144L (GTA→CTA)	gpt	xanthine‑guanine phsophoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (19/12);  total (19/12)

AAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGAC  >  NC_000913/256292‑256531                                                                                                                   |                                                                                                                              aaaGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAAtct                                                                                                             >  2:36094/1‑133 (MQ=255)       cACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTc                                                                                                       >  2:451929/1‑133 (MQ=255)              tCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATc                                                                                                >  1:524012/1‑133 (MQ=255)                  cATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTAGTCCCGCAAATTTCGGGTCGCAAATCtttt                                                                                            >  1:234027/1‑133 (MQ=255)                    tCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCa                                                                                          <  1:36094/133‑1 (MQ=255)                             aaCCGGCTGGACGTCGGCTGGTTGCTGACTATGTTGTTGATTTCCCGCAAGATACCTGGATGGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGc                                                                                 <  2:234027/133‑1 (MQ=255)                                       tcgtcCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGc                                                                       >  1:241761/1‑133 (MQ=255)                                                           aTGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAg                                                   >  1:602151/1‑133 (MQ=255)                                                            tgttgtTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAgg                                                  <  1:451929/133‑1 (MQ=255)                                                            tgttgtTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAgg                                                  <  2:524012/133‑1 (MQ=255)                                                            tgttgtTGATATCCCCCAAGATTCCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAgg                                                  <  1:266575/133‑1 (MQ=255)                                                                gttgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACACCTGGCACTGCCTGGCTTTGTTCTTTTTAACTTCAGGCggg                                              >  2:204330/1‑133 (MQ=255)                                                                 ttgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCg                                                <  2:21886/130‑1 (MQ=255)                                                                 ttgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCg                                                >  1:21886/1‑130 (MQ=255)                                                                    atatCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTAc                                          >  2:611634/1‑133 (MQ=255)                                                                      atCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAgg                                                  <  1:551017/123‑1 (MQ=255)                                                                      atCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAgg                                                  >  2:551017/1‑123 (MQ=255)                                                                      atCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACaa                                        >  2:439156/1‑133 (MQ=255)                                                                      atCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACaa                                        >  2:75030/1‑133 (MQ=255)                                                                        cccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATa                                      >  2:76026/1‑133 (MQ=255)                                                                          cGCAAGATTCCTGGATTGTACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGt                                    <  1:204330/133‑1 (MQ=255)                                                                           gCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAGCTTAAGCCGGGTTTCAATAGtt                                   >  1:243085/1‑133 (MQ=255)                                                                                         ttGAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGTGTTACAATAGTTTCCAGTAAGTATTc                     >  1:92516/1‑133 (MQ=255)                                                                                           gAACAGCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTg                   >  1:141061/1‑133 (MQ=255)                                                                                                gCCGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGc              <  2:141061/133‑1 (MQ=255)                                                                                                 ccGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAg                <  2:549784/130‑1 (MQ=255)                                                                                                 ccGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAg                >  1:549784/1‑130 (MQ=255)                                                                                                 ccGTGGGATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCt             <  1:439156/133‑1 (MQ=255)                                                                                                      ggATATGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATc        >  2:145567/1‑133 (MQ=255)                                                                                                           tGGGCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGCGGGGTTACAATAGTTTCCAGAAAGAATTCTGAAGGCTGCATCCATgc   >  1:613786/1‑132 (MQ=255)                                                                                                            gggCGTCCTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGac  <  1:611634/133‑1 (MQ=255)                                                                                                                   |                                                                                                                              AAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGAC  >  NC_000913/256292‑256531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑