Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,606,743	G→A	G161D (GGC→GAC)	hyfE →	hydrogenase 4 component E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,606,743	0	G	A	100.0%	32.7 / NA	12	G161D (GGC→GAC)	hyfE	hydrogenase 4 component E
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/8);  total (4/8)

GGGCTGAAACCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCAAATTTTTGGTTACTGCCTGATGGAAAACGGCTCCCATCTGGTGCTGGCGCTTCTTGCCTGGCGAGCACCGGAACTGGTGGAAATAGGTATCGCTACCGACGCCATCTTCGCCGTCATTGTGATGGTGTTACTGGCAAGAAAAAT  >  NC_000913/2606628‑2606857                                                                                                                    |                                                                                                                   gggCTGAAGCCGGCGATGTCGGCAGCGTTACGTCATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCAAATTTTTGGTTACTGCCTGATGGAAAACGACTCCCATCTGGTGCTgg                                                                                                   <  1:173203/133‑1 (MQ=255) gggCTGAAACCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCGAATTTTTGGTTACTGCCTGATGGAAAACGACTCCCATCTGGTGCTgg                                                                                                   <  2:373651/133‑1 (MQ=255)           cGGCGCTGGCGGTAGTGTAAGGCCATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCAAATTTTTGGTTACTGCCTGATGGAAAACGACTCCCATCTGGTGCTGGCGCTTCTTGc                                                                                         <  1:189625/133‑1 (MQ=255)           cGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCAAATTTTTGGTTACTGCCTGATGGAAAACGACTCCCATCTGGTGCTGGCGCTTCTTGc                                                                                         <  1:359015/133‑1 (MQ=255)                                    ttttCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCAAATTTTTGGTTACTGCCTGATGGAAAACGACTCCCATCTGGTGCTGGCGCTTCTTGCCTGGCGAGCACCGGAACTGGTGGaa                                                                <  1:349044/133‑1 (MQ=255)                                               gCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCAAATTTTTGGTTACTGCCTGATGGAAAACGACTCCCATCTGGTGCTGGCGCTTCTTGCCTGGCGAGCACCGGAACTGGTGGAAATAGGTATCGc                                                     >  1:430820/1‑133 (MQ=255)                                                              tCAGCCAGCGCAATATCCTGCGGCAAATTTTTGGTTACTGCCTGATGGAAAACGACTCCCATCTGGTGCTGGCGCTTCTTGCCTGGCGAGCACCGGAACTGGTGGAAATAGGTATCGCTACCGACGCCATCtt                                      >  2:553678/1‑133 (MQ=255)                                                                      cgcAATATCCTGCGGCAAATTTTTGGTTACTGGCTGATGGAAAACGACTCCCATCTGGTGCTGGCGCTTCTTGCCTGGCGAGCACCGGAACTGGTGGAAATAGGTATCGCTACCGACGCCATCTTCGCCGTCa                              >  2:428513/1‑133 (MQ=255)                                                                          atatCCTGCGGCAAAATTTTGGTTACTGCCTGATGGAAAACGACTCCCATCTGGTGCTGGCGCTTCTTGCTTGGCGAGCACCGGAACTGGTGGAAATAGGTATCGCTACCGACGCCATCTTCGCCGTCATtgt                          <  1:617481/133‑1 (MQ=255)                                                                            atCCTGCGGCGAATTTTTGGTTACGGCCGGATGGAAAACGATTCCCATCTGGTGCTGGCGCTTCTTGCCTGGCGAGCACCGGAACTGGTGGAAATAGGTATCGCTACCGACGCCATCTTCGCCGTCATTGTGa                        <  2:231881/133‑1 (MQ=255)                                                                                 gCGGCAAATTTTTGGTTACTGCCTGATGGAAAACGACTCCCATCTGGTGCTGGCGCTTCTTGCCTGGCGAGCACCGGAACTGGTGGAAATAGGTATCGCTACCGACGCCATCTTCGCCGTCATTGTGATGgtg                   >  1:95097/1‑133 (MQ=255)                                                                                                  aCTGCCTGTTGGAAATCGACTCCCATCTGGAGCTGGCGCTTCTTGCCTGGCGAGCACCGGAACTGGTGGAAATAGGTATCGCTACCGACGCCATCTTCGCCGTCATTGTGATGGTGTTACTGGCAAGAAAAat  <  1:211722/133‑1 (MQ=255)                                                                                                                    |                                                                                                                   GGGCTGAAACCGGCGCTGGCGGTAGCGTTAGGTCATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAGCGCAATATCCTGCGGCAAATTTTTGGTTACTGCCTGATGGAAAACGGCTCCCATCTGGTGCTGGCGCTTCTTGCCTGGCGAGCACCGGAACTGGTGGAAATAGGTATCGCTACCGACGCCATCTTCGCCGTCATTGTGATGGTGTTACTGGCAAGAAAAAT  >  NC_000913/2606628‑2606857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑