Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,753,762	A→C	Q53P (CAA→CCA)	bamE →	outer membrane protein assembly factor BamE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,753,762	0	A	C	100.0%	215.8 / NA	61	Q53P (CAA→CCA)	bamE	outer membrane protein assembly factor BamE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/30);  total (31/30)

CTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAA  >  NC_000913/2753641‑2753891                                                                                                                          |                                                                                                                                  ccaTTGATGTCGACCCCAGGCTGTGCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGt                                                                                                                        <  2:286149/131‑1 (MQ=255)    ttGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACg                                                                                                                     >  1:319806/1‑133 (MQ=255)    ttGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACCTGTTGGCATGACGCAACCACATGTTGCGTACg                                                                                                                     >  2:211715/1‑133 (MQ=255)       aTGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCAt                                                                                                                  >  2:380946/1‑133 (MQ=255)       aTGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCAt                                                                                                                  <  2:319806/133‑1 (MQ=255)        tGTTGACCGCAGGCTTTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTGTCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCAtt                                                                                                                 <  2:200914/133‑1 (MQ=255)        tGTTGACCCCAGGCTCTTGCACGCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCAtt                                                                                                                 <  1:253224/133‑1 (MQ=255)         gTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTg                                                                                                                >  1:127647/1‑133 (MQ=255)         gTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTg                                                                                                                >  2:8249/1‑133 (MQ=255)            gACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGt                                                                                                             >  1:101300/1‑133 (MQ=255)            gACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGt                                                                                                             >  1:342866/1‑133 (MQ=255)            gACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACAGCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGt                                                                                                             >  2:391509/1‑133 (MQ=255)             aCCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTTACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTa                                                                                                            >  2:371858/1‑133 (MQ=255)             aCCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTa                                                                                                            <  1:547391/133‑1 (MQ=255)               cGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTaca                                                                                                          <  2:63774/133‑1 (MQ=255)                  aGGCTGTTCCCCTCTGGAGCGAGTCGTTTACCCTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCg                                                                                                       <  2:193523/133‑1 (MQ=255)                  aGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCg                                                                                                       >  1:34328/1‑133 (MQ=255)                        ttCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATg                                                                                                 >  1:576770/1‑133 (MQ=255)                                       aGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGt                                                                                  <  2:560813/133‑1 (MQ=255)                                           gTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGa                                                                              <  2:101300/133‑1 (MQ=255)                                           gTTTACCGTCATGTCATCAACCAGTGGACCTACCTGACCGCCAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGa                                                                              <  2:168595/133‑1 (MQ=255)                                              tACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATa                                                                           >  2:519792/1‑133 (MQ=255)                                                 cGTCCTGACATCAACCAGAGGAACTATCTGGCCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCt                                                                        <  1:304344/133‑1 (MQ=255)                                                   tCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGTGTACACCGCTGAAGACCAATCCATTCGGTACGAATACCTgg                                                                      >  2:304344/1‑133 (MQ=255)                                                   tCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATAcc                                                                         >  1:150751/1‑130 (MQ=255)                                                   tCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATAcc                                                                         <  2:150751/130‑1 (MQ=255)                                                   tCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTgg                                                                      >  1:151120/1‑133 (MQ=255)                                                           tCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGt                                                              >  2:461012/1‑133 (MQ=255)                                                              aCCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCtt                                                           >  2:403792/1‑133 (MQ=255)                                                                   gggAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGcc                                                      <  1:391509/133‑1 (MQ=255)                                                                    ggAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCa                                                     <  2:127647/133‑1 (MQ=255)                                                                       aCTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGAACCATTTGGTACGCATATCTGGTTCTATGTCGTCCGCCAgga                                                  >  2:295231/1‑131 (MQ=255)                                                                         tATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAAc                                                <  2:34328/133‑1 (MQ=255)                                                                         tATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAAc                                                <  1:616241/133‑1 (MQ=255)                                                                             tGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAgg                                            >  2:78282/1‑133 (MQ=255)                                                                              gACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGt                                           <  1:326618/133‑1 (MQ=255)                                                                               aCCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTc                                          >  2:508237/1‑133 (MQ=255)                                                                                  gCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATg                                       <  2:387763/133‑1 (MQ=255)                                                                                   cTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGa                                      <  2:534112/133‑1 (MQ=255)                                                                                   cTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGa                                      <  1:8249/133‑1 (MQ=255)                                                                                          cgTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTATTCCGCCAGCAGCCTGGTCATCAAGGTGTa                               >  1:158973/1‑133 (MQ=255)                                                                                           gTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTaa                              <  1:519792/133‑1 (MQ=255)                                                                                           gTATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTaa                              >  2:110926/1‑133 (MQ=255)                                                                                            tATCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGATTCTATGTGTTCCGCCGGCAACCAGATCATGAAGGTGTAAc                             >  2:168940/1‑133 (MQ=255)                                                                                              tCCAAAATACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTc                           >  1:65158/1‑133 (MQ=255)                                                                                                cAAAATACATGTTGGCATGACGCAACCACACGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAg                         <  1:295231/133‑1 (MQ=255)                                                                                                  aaaTACGTGTTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCa                       <  2:342866/133‑1 (MQ=255)                                                                                                       cGTGTTGGGATGACGCAACCACAAGTTGCGGACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgc                  <  1:211715/133‑1 (MQ=255)                                                                                                        gtgtTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgct                 >  2:132811/1‑133 (MQ=255)                                                                                                        gtgtTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgct                 >  2:122837/1‑133 (MQ=255)                                                                                                        gtgtTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgct                 >  2:541269/1‑133 (MQ=255)                                                                                                        gtgtTGGCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgct                 >  2:469924/1‑133 (MQ=255)                                                                                                             ggCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgctgacgc            <  1:380946/133‑1 (MQ=255)                                                                                                             ggCATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgctgacgc            <  2:35174/133‑1 (MQ=255)                                                                                                             ggCATGACGCAACCAAAAATTGAGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgctgacgc            <  1:241536/133‑1 (MQ=255)                                                                                                               cATGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgctgacgctg          <  1:403792/133‑1 (MQ=255)                                                                                                                 tGACGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGCGTAACTCAGCAAACgctgacgctgac        >  2:587120/1‑133 (MQ=255)                                                                                                                   aCGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgctgacgc            >  2:4705/1‑127 (MQ=255)                                                                                                                   aCGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAacgctgacgc            <  1:4705/127‑1 (MQ=255)                                                                                                                   aCGCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCt      >  1:357776/1‑133 (MQ=255)                                                                                                                     gCAACCACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCttt    <  1:2177/133‑1 (MQ=255)                                                                                                                       aaccacaaGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTaa  <  1:508237/133‑1 (MQ=255)                                                                                                                       aaccacaaGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTaa  <  1:513422/133‑1 (MQ=255)                                                                                                                       aaccacaaGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTaa  <  2:554921/133‑1 (MQ=255)                                                                                                                          |                                                                                                                                  CTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTGTTGGCATGACGCAACAACAAGTTGCGTACGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAA  >  NC_000913/2753641‑2753891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑