Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,780	T→C	K744K (AAA→AAG)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,780	0	T	C	100.0%	104.6 / NA	30	K744K (AAA→AAG)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

CTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAG  >  NC_000913/2819666‑2819904                                                                                                                   |                                                                                                                             cTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTa                                                                                                            <  1:147168/133‑1 (MQ=255) cTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTa                                                                                                            <  2:376943/133‑1 (MQ=255) cTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTa                                                                                                            <  2:434298/133‑1 (MQ=255) cTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTa                                                                                                            <  1:480892/133‑1 (MQ=255) cTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTa                                                                                                            <  1:307007/133‑1 (MQ=255)    aaCACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGt                                                                                                         >  1:69156/1‑133 (MQ=255)    aaCACCGCCAAGCTCGCTTACCCACAGCTTTACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGt                                                                                                         <  1:182078/133‑1 (MQ=255)                   gCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAgcgc                                                                                          <  1:11768/133‑1 (MQ=255)                    cTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCa                                                                                         <  1:416744/133‑1 (MQ=255)                    cTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCa                                                                                         <  2:43818/133‑1 (MQ=255)                               cTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGcc                                                                              <  1:567020/133‑1 (MQ=255)                                    cacCATTAACATCAATTGCCTTACTGGAAAGATTAGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGatt                                                                         >  2:339813/1‑133 (MQ=255)                                              aTCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCg                                                               >  2:505115/1‑133 (MQ=255)                                              aTCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCg                                                               >  2:318647/1‑133 (MQ=255)                                                   ttGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCttt                                                          >  2:448087/1‑133 (MQ=255)                                                   ttGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGAACTGAGCGGACTTTATCAGCCAGATCATTGCTATCGCCttt                                                          >  2:269620/1‑133 (MQ=255)                                                       cTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTcagc                                                      >  2:375859/1‑133 (MQ=255)                                                           cTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGAt                                                  >  1:531137/1‑133 (MQ=255)                                                            tGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATg                                                 >  1:504691/1‑133 (MQ=255)                                                                             tcCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGGAGACGCGCGACTTAGCTTAAGc                                >  1:251209/1‑133 (MQ=255)                                                                             tcCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGc                                >  1:64327/1‑133 (MQ=255)                                                                                  tgCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCg                           <  1:339813/133‑1 (MQ=255)                                                                                  tgCGGCAGCTTGTTCTTAAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTATCAACAGCTGCGCGACTTCGCGTAAGCGATCg                           >  2:245953/1‑133 (MQ=255)                                                                                    cGGCAGCTTGCTCTTAAAGCTGTTTTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCt                         <  2:251209/133‑1 (MQ=255)                                                                                       cAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTc                      >  2:554820/1‑133 (MQ=255)                                                                                       cAGCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTc                      <  2:64327/133‑1 (MQ=255)                                                                                         gCTTGTTCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTg                    >  2:336922/1‑133 (MQ=255)                                                                                              ttCTTTAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGa               <  1:554820/133‑1 (MQ=255)                                                                                                   tAAGCTGTTGTAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGt          <  2:531137/133‑1 (MQ=255)                                                                                                           tgtAACTCCTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGTTGTCTGCACGAACGGTGGCGATAg  >  2:230524/1‑133 (MQ=255)                                                                                                                   |                                                                                                                             CTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAG  >  NC_000913/2819666‑2819904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑