Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,817	C→A	intergenic (‑241/‑15)	mltB ← / → srlA	membrane‑bound lytic murein transglycosylase B/sorbitol‑specific PTS enzyme IIC2 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,817	0	C	A	100.0%	173.8 / NA	50	intergenic (‑241/‑15)	mltB/srlA	membrane‑bound lytic murein transglycosylase B/sorbitol‑specific PTS enzyme IIC2 component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/26);  total (24/26)

CCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGC  >  NC_000913/2825689‑2825939                                                                                                                                 |                                                                                                                           cccTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGaa                                                                                                                        <  2:624932/133‑1 (MQ=255)    tCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGga                                                                                                                     >  2:369291/1‑133 (MQ=255)    tCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGga                                                                                                                     <  2:494214/133‑1 (MQ=255)    tCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGga                                                                                                                     <  2:27154/133‑1 (MQ=255)         aTCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGaa                                                                                                                <  1:338405/133‑1 (MQ=255)            cTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACaa                                                                                                             <  1:358421/133‑1 (MQ=255)               ttATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACaa                                                                                                             >  2:416497/1‑130 (MQ=255)               ttATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACaa                                                                                                             <  1:416497/130‑1 (MQ=255)                tATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAATTCATGAGGGAGAGAACAATGAt                                                                                                         >  1:255238/1‑133 (MQ=255)                tATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGAt                                                                                                         >  1:612519/1‑133 (MQ=255)                tATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGAt                                                                                                         >  1:481224/1‑133 (MQ=255)                tATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGAt                                                                                                         >  2:419021/1‑133 (MQ=255)                  ttCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAg                                                                                                       >  1:357534/1‑133 (MQ=255)                   tCAAACTTTCAAATTTAAATATTTATGTCTCAATTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGa                                                                                                      <  2:277336/133‑1 (MQ=255)                   tCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGa                                                                                                      <  2:148494/133‑1 (MQ=255)                   tCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGa                                                                                                      <  1:500918/133‑1 (MQ=255)                      aaCTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAAc                                                                                                   >  2:100822/1‑133 (MQ=255)                       aCTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGA‑AACAATGATAGAAACCa                                                                                                 <  2:135210/133‑1 (MQ=255)                                    aaTATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTg                                                                                     <  1:590175/133‑1 (MQ=255)                                    aaTATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTg                                                                                     <  2:132412/133‑1 (MQ=255)                                    aaTATTTATCTTTCAGTTTGCGAACATATTAAGACTTTTAATTCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTg                                                                                     <  2:303468/133‑1 (MQ=255)                                       atTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCag                                                                                  <  1:435072/133‑1 (MQ=255)                                           aTCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACGATGGTAGGAACCATTACTCATTGTGCAGAGTg                                                                              >  2:171612/1‑133 (MQ=255)                                                tCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTa                                                                         >  2:577782/1‑133 (MQ=255)                                                  aTTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATc                                                                       >  1:87930/1‑133 (MQ=255)                                                    tttGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCgg                                                                     <  1:100822/133‑1 (MQ=255)                                                      tGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCggg                                                                    <  1:626340/132‑1 (MQ=255)                                                      tGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCggg                                                                    >  2:626340/1‑132 (MQ=255)                                                      tGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGc                                                                   >  2:519373/1‑133 (MQ=255)                                                      tGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGc                                                                   <  2:87930/133‑1 (MQ=255)                                                      tGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGc                                                                   >  2:339489/1‑133 (MQ=255)                                                           tCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTc                                                              <  1:369291/133‑1 (MQ=255)                                                              aaaTAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCaa                                                           <  1:577782/133‑1 (MQ=255)                                                                     acTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCg                                                    <  1:519373/133‑1 (MQ=255)                                                                       ttttAAATCTTTCAATCTGATTAGATTAGGGTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGga                                                  <  1:251938/133‑1 (MQ=255)                                                                         ttAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGgaga                                                >  2:92615/1‑133 (MQ=255)                                                                         ttAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGgaga                                                >  2:372240/1‑133 (MQ=255)                                                                         ttAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGgaga                                                >  1:511475/1‑133 (MQ=255)                                                                         ttAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGgaga                                                >  1:461267/1‑133 (MQ=255)                                                                         ttAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGgaga                                                >  1:17231/1‑133 (MQ=255)                                                                         ttAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGCGGTTTATCGGGCTGTTCCAAAAGGGCGgaga                                                >  1:538724/1‑133 (MQ=255)                                                                                   cAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTAcc                                      <  2:461267/133‑1 (MQ=255)                                                                                     aTCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCgg                                    >  1:27199/1‑133 (MQ=255)                                                                                         gattagattaGGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATg                                <  2:511475/133‑1 (MQ=255)                                                                                               attagGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAcc                          >  1:390921/1‑133 (MQ=255)                                                                                                 tagGTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCgg                        <  1:396631/133‑1 (MQ=255)                                                                                                    gTTGCCGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAt                     >  1:587942/1‑133 (MQ=255)                                                                                                         cGTTTGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttcttc                >  2:604593/1‑133 (MQ=255)                                                                                                             tGGTAATAAAACAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCt            <  2:587942/133‑1 (MQ=255)                                                                                                                       aCAATAAATCATGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGc  <  1:339489/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                           CCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGC  >  NC_000913/2825689‑2825939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑