Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,829,365	G→A	V107I (GTT→ATT)	srlR →	DNA‑binding transcriptional repressor SrlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,829,365	0	G	A	100.0%	128.7 / NA	38	V107I (GTT→ATT)	srlR	DNA‑binding transcriptional repressor SrlR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/19);  total (19/19)

CGCCTATCGATCATAAAACACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAAT  >  NC_000913/2829240‑2829491                                                                                                                              |                                                                                                                               cgCCTATCGATCATAAAACACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCt                                                                                                                         >  1:373564/1‑133 (MQ=255)        cGATCATAAAACACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTcg                                                                                                                  <  1:603502/133‑1 (MQ=255)        cGATCATAAAACACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTcg                                                                                                                  <  1:397201/133‑1 (MQ=255)          aTCATAAAACACTCAGCACCACGCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTcgcg                                                                                                                <  1:272964/133‑1 (MQ=255)            cATAAAACACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCt                                                                                                              >  2:597762/1‑133 (MQ=255)             aTAAAACACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCtt                                                                                                             >  1:4438/1‑133 (MQ=255)              tAAAACACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCttt                                                                                                            >  1:350656/1‑133 (MQ=255)                aaaCACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTtaa                                                                                                          <  2:321747/133‑1 (MQ=255)                aaaCACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTtaa                                                                                                          <  1:77581/133‑1 (MQ=255)                 aaCACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTtaat                                                                                                         <  1:20223/133‑1 (MQ=255)                      tcatcaACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGACGATTCCCCTGCTCTCGCGCTTTAATAACAt                                                                                                    >  1:265855/1‑133 (MQ=255)                        atcaACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCa                                                                                                  >  1:381546/1‑133 (MQ=255)                        atcaACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCa                                                                                                  >  2:523500/1‑133 (MQ=255)                        atcaACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCa                                                                                                  >  1:491168/1‑133 (MQ=255)                        atcaACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGATTTGCAGACGATTCCCATGCTCTCGGGCTTTAATAACATCa                                                                                                  >  2:202344/1‑133 (MQ=255)                               cccACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGtgat                                                                                           <  2:4438/133‑1 (MQ=255)                               cccACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGtgat                                                                                           <  2:381546/133‑1 (MQ=255)                                    aagaaagaGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCa                                                                                      <  2:373564/133‑1 (MQ=255)                                    aagaaagaGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCa                                                                                      <  2:350656/133‑1 (MQ=255)                                              tGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCa                                                                            >  2:395223/1‑133 (MQ=255)                                                    cAGAAGCTTCCGTTACTTTTATCCATGATGTCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGt                                                                      <  1:170143/133‑1 (MQ=255)                                                     aGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTc                                                                     >  2:243216/1‑133 (MQ=255)                                                          cTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATgc                                                                <  1:482092/133‑1 (MQ=255)                                                           tGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATgcg                                                               <  1:395223/133‑1 (MQ=255)                                                             ccGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCt                                                             >  2:464258/1‑133 (MQ=255)                                                                       tATCCATGATGGCGATTCGATCATTCTTGATGCCGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATAGCGTCAATGCGCTATCCGAACTg                                                   >  1:280085/1‑133 (MQ=255)                                                                              gatgGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCGTATGGTCAATGCGCTAGCCGAACTGGATAACg                                            >  2:227501/1‑133 (MQ=255)                                                                              gatgGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTAGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACg                                            >  2:391276/1‑133 (MQ=255)                                                                               atgGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGa                                           <  1:563262/133‑1 (MQ=255)                                                                                     aTTCGATCTTTCTTGATGCTGGTAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAAc                                     <  1:202344/133‑1 (MQ=255)                                                                                      ttCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACt                                    <  1:597762/133‑1 (MQ=255)                                                                                                cTTGATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGc                          <  2:265855/133‑1 (MQ=255)                                                                                                   gATGCTGGCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAg                       >  1:597087/1‑133 (MQ=255)                                                                                                         ggCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAATAAACTATCCTGATTCCAGGCGGaa                 >  2:230862/1‑133 (MQ=255)                                                                                                         ggCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGaa                 >  2:330328/1‑133 (MQ=255)                                                                                                          gCAGTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAAc                >  1:616144/1‑133 (MQ=255)                                                                                                             gTACCGTTTTGCAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGtt             <  1:243216/133‑1 (MQ=255)                                                                                                                        cAGATGATTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAAt  <  2:491168/133‑1 (MQ=255)                                                                                                                              |                                                                                                                               CGCCTATCGATCATAAAACACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAAT  >  NC_000913/2829240‑2829491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑