Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,842,850 T→G D65A (GAT→GCT)  hycI ← hydrogenase 3 maturation protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,842,8500TG100.0% 158.5 / NA 44D65A (GAT→GCT) hycIhydrogenase 3 maturation protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/15);  total (29/15)

CCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTT  >  NC_000913/2842727‑2842973
                                                                                                                           |                                                                                                                           
ccAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGt                                                                                                                    >  2:447794/1‑133 (MQ=255)
   atatCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGa                                                                                                                 <  2:367934/133‑1 (MQ=255)
             tCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATcagcag                                                                                                       >  1:415478/1‑133 (MQ=255)
                aCTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCGCATAGCCATGGTGTAGACAATAAGCAGTCg                                                                                                    >  1:183547/1‑133 (MQ=255)
                aCTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCg                                                                                                    >  2:492516/1‑133 (MQ=255)
                aCTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCg                                                                                                    >  1:568959/1‑133 (MQ=255)
                aCTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCg                                                                                                    >  1:540097/1‑133 (MQ=255)
                aCTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCAGGGTTTAGACCCATAGGCGTGGCGTGGACAGTCAGCAGTCg                                                                                                    >  2:167360/1‑133 (MQ=255)
                    gTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTc                                                                                                >  2:572215/1‑133 (MQ=255)
                         tAAGGTAATTCAACGGCATGTTATTGGTTGTCCTCATAAACTTCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGgcg                                                                                           <  2:193094/133‑1 (MQ=255)
                         tAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCTATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGgcg                                                                                           <  2:540097/133‑1 (MQ=255)
                                aTTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCa                                                                                    >  2:342836/1‑133 (MQ=255)
                                 ttCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCAc                                                                                   >  1:394689/1‑133 (MQ=255)
                                      cGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATa                                                                              >  2:480870/1‑133 (MQ=255)
                                            gTTATGGGTAGTCATGATTAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGcgacg                                                                        <  1:233013/133‑1 (MQ=255)
                                            gTTATGGGAAGTCATCATCAACCTCTCGGCCATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGcgacg                                                                        <  1:167360/133‑1 (MQ=255)
                                             ttATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGcgacga                                                                       <  1:492516/133‑1 (MQ=255)
                                               aTGGGTAGTCATCATAAACATCTGGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATg                                                                     <  1:306857/133‑1 (MQ=255)
                                               aTGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATg                                                                     <  1:572215/133‑1 (MQ=255)
                                                  ggTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCg                                                                  >  1:312434/1‑133 (MQ=255)
                                                      gTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGtttt                                                              >  1:401215/1‑133 (MQ=255)
                                                       tcatcaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGTCGCGGTTCACGGATAGAGACGGTGTCGTTTTc                                                             >  2:188688/1‑133 (MQ=255)
                                                       tcatcaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTc                                                             >  1:307937/1‑133 (MQ=255)
                                                       tcatcaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTc                                                             >  1:383990/1‑133 (MQ=255)
                                                       tcatcaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGGCGTGTCGGGCGCAGGTCACGGATAGCGACGACGTCGATTTc                                                             >  2:280176/1‑133 (MQ=255)
                                                          tcatAAACATGTCGGAGATATCATCCGGCTCGCTGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCgg                                                          <  2:183547/133‑1 (MQ=255)
                                                             tAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTgc                                                       >  1:80238/1‑133 (MQ=255)
                                                                 cATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCAAGGATAGCGACGATGTCATTTTACGGTGCGCTa                                                   >  2:242072/1‑133 (MQ=255)
                                                                    ctcGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTAccg                                                <  2:308026/133‑1 (MQ=255)
                                                                     tcGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTAccgc                                               >  1:486013/1‑133 (MQ=255)
                                                                     tcGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGGTTTCCGGTGGGCTAccgc                                               >  1:169399/1‑133 (MQ=255)
                                                                         cGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGTGACGATGTCGTTTTCCGGTGCGCTACCGCCGTc                                           <  2:568959/133‑1 (MQ=255)
                                                                         cGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTc                                           <  1:480870/133‑1 (MQ=255)
                                                                         cGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTc                                           <  2:420593/133‑1 (MQ=255)
                                                                            tatCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGGGTCGGGCGCAGTTCACGGATAACGACGATGTCGTTTACCGATGAGCTACCGCCGTCaaa                                        >  1:299673/1‑132 (MQ=255)
                                                                                atcCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATcacc                                    <  2:383990/133‑1 (MQ=255)
                                                                                 tcCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATcacca                                   <  2:394689/133‑1 (MQ=255)
                                                                                    gggTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGAGGATGTCGTCTTCCGGTACGCTACCGGCGTCCATCACCAccc                                >  2:225170/1‑133 (MQ=255)
                                                                                     ggTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCa                               >  2:11241/1‑133 (MQ=255)
                                                                                      gTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGGTGTCGTTTACCGCTGCGCTACCGCCATCAATCACCACCCAg                              >  2:191737/1‑133 (MQ=255)
                                                                                          atgatgCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTAc                          >  1:136520/1‑133 (MQ=255)
                                                                                          atgatgCGGATCTCGCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTAc                          >  1:562168/1‑133 (MQ=255)
                                                                                                        gCCGGGGTTTAGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTcggcgcggcg            >  2:480598/1‑133 (MQ=255)
                                                                                                                  aGCCCCATAGCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACtttt  >  1:611380/1‑133 (MQ=255)
                                                                                                                           |                                                                                                                           
CCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCACCACCCAGTTACCTTTCGGCGCGGCGGCGCACTTTT  >  NC_000913/2842727‑2842973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑