Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,733	T→G	F8C (TTC→TGC)	ygcG →	protein YgcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,733	0	T	G	100.0%	119.9 / NA	34	F8C (TTC→TGC)	ygcG	protein YgcG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/16);  total (18/16)

TCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTA  >  NC_000913/2905603‑2905861                                                                                                                                   |                                                                                                                                 tCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCa                                                                                                                                >  2:477208/1‑133 (MQ=255) tCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCa                                                                                                                                >  2:512160/1‑133 (MQ=255) tCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTAAAATGCGCTATTTCATACTAATGTGCa                                                                                                                                <  1:621846/133‑1 (MQ=255)            atatTTATTCGTTCTCTATTAGTTTTCAGTGACAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGt                                                                                                                     <  2:300128/133‑1 (MQ=255)                  atcttttCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCttt                                                                                                               >  1:25899/4‑133 (MQ=255)                        tCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCa                                                                                                         <  1:512160/133‑1 (MQ=255)                           gTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGcc                                                                                                      <  1:135564/133‑1 (MQ=255)                           gTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGcc                                                                                                      >  1:518659/1‑133 (MQ=255)                           gTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGcc                                                                                                      <  1:477208/133‑1 (MQ=255)                            tATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATTTGCACTTTCGTATGTTCCTTTGTTGCAGcc                                                                                                      <  1:516810/132‑1 (MQ=255)                            tATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATTTGCACTTTCGTATGTTCCTTTGTTGCAGcc                                                                                                      >  2:516810/1‑132 (MQ=255)                                 gTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCccaacca                                                                                                >  1:442719/1‑133 (MQ=255)                                     ttAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCTAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTa                                                                                            <  2:379480/133‑1 (MQ=255)                                      tAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGAATGTTCCTTTGGTGAAGCCCATCCAACTAt                                                                                           >  1:268473/1‑133 (MQ=255)                                           aTAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTcc                                                                                      <  1:481772/133‑1 (MQ=255)                                           aTAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTcc                                                                                      <  2:25899/133‑1 (MQ=255)                                           aTAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTcc                                                                                      <  2:342085/133‑1 (MQ=255)                                                                aTGCCGCCGTCAATTTATTTATCCAGATTTAATCAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTa                                                                 <  2:10846/133‑1 (MQ=255)                                                                  gccgccGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACg                                                               <  1:86144/133‑1 (MQ=255)                                                                        gTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCtt                                                         <  2:518659/133‑1 (MQ=255)                                                                                atttatCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAg                                                 >  1:452726/1‑133 (MQ=255)                                                                                     tCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTaa                                            >  2:480854/1‑133 (MQ=255)                                                                                      ccAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTaaa                                           >  2:580997/1‑133 (MQ=255)                                                                                                tGAGAAATTACTAATGCGCTATTTTATACTAATGTGCACTTCCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACaagaa                                 <  2:268473/133‑1 (MQ=255)                                                                                                    aaaTTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGGTCTTACGGGTAGCTTAGACTCACaagaaaaga                             >  2:261660/1‑133 (MQ=255)                                                                                                    aaaTTTATAATGCGCTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACaagaaaaga                             >  2:596214/1‑133 (MQ=255)                                                                                                               gcgcTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACg                  <  2:452726/133‑1 (MQ=255)                                                                                                                  cTATTTCATACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGcac               <  1:596214/133‑1 (MQ=255)                                                                                                                          tACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGa       >  2:464079/1‑133 (MQ=255)                                                                                                                          tACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGa       >  2:453539/1‑133 (MQ=255)                                                                                                                          tACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGa       >  1:98708/1‑133 (MQ=255)                                                                                                                          tACTAATGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAAAAATGAAAGAACAGACGAACAAGTTAGa       >  2:250735/1‑133 (MQ=255)                                                                                                                               aTGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAGCTCACAAGGAAAAAAAGAACTGACGAACAAATTAGAATCTa  >  2:187763/1‑133 (MQ=255)                                                                                                                               aTGTGCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTa  >  1:122075/1‑133 (MQ=255)                                                                                                                                   |                                                                                                                                 TCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTA  >  NC_000913/2905603‑2905861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑