Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,008,544	C→T	G345G (GGC→GGT)	ygeX →	2,3‑diaminopropionate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,008,544	0	C	T	100.0%	77.5 / NA	27	G345G (GGC→GGT)	ygeX	2,3‑diaminopropionate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

TGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACG  >  NC_000913/3008422‑3008653                                                                                                                           |                                                                                                              tGCGCCACCCTATTCGTCTCCTGCAAGGCCAGCGTTGCCGCGTTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAg                                                                                                     <  2:224285/133‑1 (MQ=255) tGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAg                                                                                                     <  2:513028/133‑1 (MQ=255)    gcCACCCATTTCATCTCCCGCCTGGACAGCCTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCgg                                                                                                  <  1:158618/133‑1 (MQ=255)     ccACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGt                                                                                                 >  2:490674/1‑133 (MQ=255)     ccACCCAATTAATCTGCTGCCAGGACAGCGTTCCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGt                                                                                                 <  2:251756/133‑1 (MQ=255)      cACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGtt                                                                                                >  1:320495/1‑133 (MQ=255)        cccAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGTGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGATGTTCTCGCGGCGGTTCa                                                                                              >  1:265937/1‑133 (MQ=255)        cccAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGAGCTTTCGGTTTGGGTGGTCTCGCAGCGGTTCa                                                                                              >  2:239350/1‑133 (MQ=255)        cccAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGGGAATCCGGGGCTGTGGGTTTGGGTGTTCTCTCAGCGGttga                                                                                              >  2:299327/1‑131 (MQ=255)         ccAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCAt                                                                                             >  2:96465/1‑133 (MQ=255)                  ctcCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCGGCGGTTCATCATCAcctg                                                                                    >  1:213595/1‑131 (MQ=255)                            gACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCCCAGCGGTTCATAATCACCCGCAACGTCaaa                                                                          >  1:212884/1‑133 (MQ=255)                                 cGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCCGTTCATTATCACCCGCAACGTCAAAGCCTg                                                                     >  1:272072/1‑133 (MQ=255)                                                tATGCGCGTTGTGGGCAATCCGTAAGGCAACGGCCCGCGCAGCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGgcg                                                      <  2:272072/133‑1 (MQ=255)                                                         gCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACaaa                                             >  1:394777/1‑133 (MQ=255)                                                         gCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAACCCCGATGGAAAAACTGGCGCTGAACaaa                                             >  2:265384/1‑133 (MQ=255)                                                           tGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGa                                           <  1:96465/133‑1 (MQ=255)                                                                aaTCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCg                                      <  1:490674/133‑1 (MQ=255)                                                                 aTCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCgt                                     <  1:375292/133‑1 (MQ=255)                                                                       aCGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCt                               >  2:448002/1‑133 (MQ=255)                                                                           cAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGtt                           >  2:448750/1‑133 (MQ=255)                                                                                aCCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAg                      >  1:71917/1‑133 (MQ=255)                                                                                     cgcATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTg                 <  1:76154/133‑1 (MQ=255)                                                                                     cgcATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTg                 >  2:578062/1‑133 (MQ=255)                                                                                            tctcCGGTGAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGT‑GTGCTGGTTATCAGCAGTGAAGGCGac         >  2:212519/1‑133 (MQ=255)                                                                                            tctcCGGTAAATCCGACGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGa          <  1:299327/133‑1 (MQ=255)                                                                                                    gAATCCGGCGCTGTCGGTTTGGGTGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACg  <  1:578062/133‑1 (MQ=255)                                                                                                                           |                                                                                                              TGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGGCGCTGTCGGTTTGGGCGTTCTCGCAGCGGTTCATTATCACCCGCAACGTCAAAGCCTGATGGAAAAACTGGCGCTGAACAAAGATGCCGTGGTGCTGGTTATCAGCACTGAAGGCGACACCGACG  >  NC_000913/3008422‑3008653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑