Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,157	T→C	intergenic (‑77/+16)	yggE ← / ← argO	uncharacterized protein YggE/L‑arginine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,157	0	T	C	100.0%	128.8 / NA	37	intergenic (‑77/+16)	yggE/argO	uncharacterized protein YggE/L‑arginine exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/20);  total (17/20)

CCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTG  >  NC_000913/3068034‑3068279                                                                                                                            |                                                                                                                            ccGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGa                                                                                                                    <  2:614254/133‑1 (MQ=255)    cTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAg                                                                                                                 >  2:547116/1‑133 (MQ=255)    cTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCGTAGCGCCTCTGTTTTATTTCTCAATCAGATAg                                                                                                                 >  2:277220/1‑133 (MQ=255)      aaTACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAgcg                                                                                                               <  2:501181/133‑1 (MQ=255)         aCCCATTATTGCCGCCAGGGCGGTAACTTTCAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCt                                                                                                            <  1:202017/133‑1 (MQ=255)           ccATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTaa                                                                                                          <  2:594566/133‑1 (MQ=255)               tAATGTCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGa                                                                                                      >  1:34309/1‑133 (MQ=255)               tAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGa                                                                                                      >  2:590013/1‑133 (MQ=255)                aaTGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGaa                                                                                                     <  1:336965/133‑1 (MQ=255)                  tGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACa                                                                                                   <  1:592771/133‑1 (MQ=255)                       ccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCGCCATCAGATAGCGCCTAACTGTACAAGGCt                                                                                              >  1:273064/1‑133 (MQ=255)                            gCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                         <  1:547116/133‑1 (MQ=255)                                      tGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATa                                                                              >  1:84290/1‑133 (MQ=255)                                      tGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATa                                                                              >  2:90237/1‑133 (MQ=255)                                      tGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATa                                                                              >  2:119738/1‑133 (MQ=255)                                             cACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGtctc                                                                       <  1:117921/133‑1 (MQ=255)                                              aCGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTAGTGAATGA‑GCAATGCCGtctct                                                                      >  1:190749/1‑133 (MQ=255)                                                 ttAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGc                                                                   <  1:119738/133‑1 (MQ=255)                                                  tAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGcc                                                                  >  1:340102/1‑133 (MQ=255)                                                       tctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCATGTGCATAA‑GCAATACAGTCTGTCGCCAGCTg                                                             >  2:173621/1‑133 (MQ=255)                                                         tcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCa                                                           <  2:84290/133‑1 (MQ=255)                                                         tcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTACATGA‑GCGATAGCGGCTCTCGACAGCTGCa                                                           >  1:160380/1‑133 (MQ=255)                                                          ccGTGTTAAGCGACTCGCCCAAAATTCAGGACATCTGGAGGAAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCaa                                                          <  2:273064/133‑1 (MQ=255)                                                              gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                      <  1:90237/133‑1 (MQ=255)                                                                  aTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAgg                                                        <  1:581240/127‑1 (MQ=255)                                                                  aTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAgg                                                        >  2:581240/1‑127 (MQ=255)                                                                   ttGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                 <  1:127384/133‑1 (MQ=255)                                                                   ttGACTTGCCCAAAACTCAGGACCTCTGCAGGTAAGCTTAGGGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                 <  2:190749/133‑1 (MQ=255)                                                                       cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGTTGCAAGGCAATAAACcac                                             >  1:270510/1‑133 (MQ=255)                                                                           cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATaa                                         >  2:620781/1‑133 (MQ=255)                                                                              aaaaTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                      <  1:590013/133‑1 (MQ=255)                                                                                  ttCAGGACCTCTGGACGCTAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                  <  1:252531/133‑1 (MQ=255)                                                                                     aGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCcac                               <  1:620781/133‑1 (MQ=255)                                                                                           tctGGAGGCACGCTTCGCGCCTCTTTTTAATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAg                         <  2:160380/133‑1 (MQ=255)                                                                                                      gCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAAt                >  1:478553/1‑131 (MQ=255)                                                                                                      gCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAAt                <  2:478553/131‑1 (MQ=255)                                                                                                            cgcCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgtg        >  1:53314/1‑133 (MQ=255)                                                                                                                 cggTTTAATTTCTCCTTCAGATAGCGCTAAATTGAACAGGCGTTGT‑CAAGAGGCATTCCCGTCCCTCGCCGGCTGCAAGCCAA‑AAACCCCAAAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTg  <  2:187423/131‑1 (MQ=255)                                                                                                                            |                                                                                                                            CCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGA‑GCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTG  >  NC_000913/3068034‑3068279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑